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Angewandte Chemie International Edition ,
doi: 10.1002/anie.201502287

Nr. 22/2015
June 18, 2015

Contrast Agents for Imaging Cartilage

NIR Fluorophores Enable Specific Visualization of Cartilage Tissue

Contact: Hak Soo Choi, Harvard University (USA)
Registered journalists may download the original article here:
Cartilage-Specific Near-Infrared Fluorophores for Biomedical Imaging

The mainstream techniques for visualization of cartilage tissue in the body are magnetic resonance imaging and computer tomography, but both techniques do not provide optimum quality images. A new method projected by American and South Korean scientists is based on contrast agents that can be detected in the near-infrared (NIR) wavelength range. Injection of the fluorophores allows high-quality visualization of cartilage tissue in real time, as the authors report in the journal Angewandte Chemie.

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Using NIR fluorophores for tissue imaging has several advantages. Hak Soo Choi from Harvard Medical School, Boston and Hoon Hyun from Chonnam National University, South Korea explain: "The most important advantage of using NIR fluorophores for cartilage imaging is the integrated method of performing ‘structure-inherent targeting’ (i.e. molecular targeting and fluorescent imaging) using a single small molecule. The high affinity of cationic NIR fluorophores for cartilage provides high-quality tissue visualization, ultralow background signal, and low injected dose". Therefore, the Choi group in collaboration with Maged Henary at Georgia State University, Atlanta, screened an NIR fluorophore library for their cartilage specificity. The best fluorophores were based on a polymethine core with quaternary ammonium cations. They were re-synthesized and chemically altered to fine-tune their physicochemical properties. Two highly promising molecules were then successfully tested in mouse as well as Yorkshire pig models for their tissue-imaging properties.

Although it is not very clear yet what the actual binding target of the contrast agents is, the scientists believe that the highly negatively charged components of the cartilage tissue are the targets that are recognized and specifically bound by the positively charged fluorophores. In addition, the new NIR fluorophores are very stable and not degraded in the body, and the strong cartilage signal was obtained 1-4 hours after the injection of a single dose. Furthermore, the NIR fluorophores also offer the possibility of detecting cartilage and bone tissue simultaneously in real time by a dual-NIR-channel method based on the recently developed bone-specific NIR fluorophores (Hyun et al., Angew. Chem. Int. Ed. 2014) in combination with the cartilage-specific NIR fluorophores.

NIR fluorophores not only have advantages over the established mainstream imaging techniques, but also surpass other methods based on other contrast agents, as Choi explains: "NIR fluorophores provide high spatial resolution in real time to distinguish the boundary and thickness of cartilage tissues from the adjacent bones, where other contrast agents with nanoparticle formulations have limited penetration and delineation". Thus, the new NIR cartilage-specific fluorophores may be highly beneficial in many applications including investigation of cartilage degeneration, new drug design, and image-guided surgery.

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About the Author

Hak Soo Choi is Associate Professor of Medicine at Harvard Medical School in Boston. His laboratory focuses on the development of novel contrast agents for tissue- and organ-specific targeting, imaging, and diagnosis. Hoon Hyun is Assistant Professor of Chonnam National University Medical School and expert in drug delivery and NIR fluorescence imaging. Maged Henary is Assistant Professor of Chemistry, Georgia State University, Atlanta. His group is interested in the design, synthesis, and development of pharmaceutical agents for therapeutic use and imaging technology, such as cancer imaging dyes, invisible markers, and fluorescent probes.