1. This review provides a concise summary of the single-cell RNA sequencing technologies.
2. Overview and guidelines for planning experimental procedures are presented.
3. Bioinformatics tools for scRNA-seq data analysis are thoroughly discussed.
4. Applications and further development of scRNA-seq technology are highlighted.

1. Spatial omics is transforming our understanding of the cancer ecosystem at the systemic level.

2. The integration of spatial omics and single-cell omics can fundamentally improve our understanding of tumourigenesis and cancer microenvironment.

3. Generating the spatial atlas of human cancers across multiple omics and timescales will potentially pioneer the revolution of spatiotemporal molecular medicine.

1) Single-cell RNA-seq of organ-specific metastasis (liver, peritoneum, ovary, lymph node) in GC was conducted.

2) Single-cell analysis determined phenotypes and functions of four malignant epithelial subtypes in GC.

3) A 20-gene signature of lymph node-derived exhausted CD8⁺ T cells might forecast lymph node metastasis, and targeting HLA-E-KLRC1/KLRC2 signaling severs as a novel clinical therapeutic opportunity for GC.

Midbrain samples of Parkinson's disease (PD) and control (CTR) patients were subjected to a multi-omic analysis. The expression of miRNAs, transcripts and proteins was explored, followed by data integration and functional analyses. This comprehensive assessment of PD-affected and CTR human midbrains revealed multiple molecular targets and networks that are relevant to the disease mechanism of advanced PD.

1. Upregulation of SLC7A11 promotes hepatoblastoma tumorigenesis by enhancing ferroptosis resistance.
2. METTL3/IGF2BP1/m6A modification enhances the SLC7A11 mRNA stability and expression via inhibiting the deadenylation in an m6A-dependent manner.
3. The competitive binding of IGF2BP1 blocks PABPC1 from recruiting the BTG2/CCR4-NOT complex, thereby suppressing the deadenylation of SLC7A11 mRNA.

Our study compared the single-cell transcriptomes of immune cells from 12 species. The results revealed the heterogeneity and compositions of circulating immune cells. We explored the conserved and divergent cellular crosstalks and genetic regulatory networks among vertebrate immune cells.