Inflammatory programmed cell death pyroptosis executed by the pore-forming protein gasdermin D (GSDMD) is an essential step of neuroinflammation after spinal cord injury. We demonstrated that CD73, a widely accepted immunosuppressive molecule, can inhibit pyroptosis via mediating GSDMD through PI3K/AKT/Foxo1 signaling.

Menstrual blood-derived MSC transplantation significantly lowers the mortality of severe and critical SARS-CoV-2-induced COVID-19.

This prospective and systematic report assessed the ability of menstrual blood-derived MSCs to treat both severe and critically ill COVID-19 patients.

MSC-based therapy may serve in future clinical applications as an alternative way for the treatment of COVID-19

1. Gasdermins are executors of pyroptosis, which cause inflammatory cell death, accompanied with various inflammatory mediators.
2. Pyroptosis plays a dual role in tumor microenvironment, including anti-tumor and pro-tumor effects.
3. Pyroptosis can be used as a new target to fight against the tumor.

FTO stimulates tumor growth of bladder cancer through regulating MALAT1 methylation.

MALAT1 and MAL2 interact with miR-384 for regulating tumor growth of bladder cancer.

FTO promotes bladder cancer tumor growth via MALAT1/miR-384/MAL2 axis.

1. USP35 is abundant in human lung cancer tissues and cell lines.

2. USP35 modulates iron homeostasis and ferroptosis in lung cancer tissues and cell lines.

3. USP35 directly interacts with ferroportin and maintain its protein stability to prevent iron overload and ferroptosis.

• m⁷G tRNA modification and its catalytic enzyme METTL1 are elevated in hepatocellular carcinoma (HCC) and associated with poor HCC prognosis.
• The m⁷G tRNAs are essential for tRNA expression and mRNA translation.
• Overactive m⁷G tRNA expression promotes hepatocarcinogenesis in vitro and in hydrodynamic transfection HCC mouse models.

Recent studies have closely associated exosomal microRNAs (miRNAs) with various human diseases, including diabetes mellitus (DM), which is a complex multifactorial metabolic disorder disease. In the diabetic condition, exosomal miRNAs are taken up by recipient cells, where they exert their biological function and thereby modulate the progression of DM-associated complications, including diabetic retinopathy (DR), diabetic macrovascular complications (DMCs), diabetic nephropathy (DN), diabetic foot ulcer (DFU), diabetic peripheral neuropathy (DPN), and diabetic cardiomyopathy (DCM).

1. This review provides a concise summary of the single-cell RNA sequencing technologies.
2. Overview and guidelines for planning experimental procedures are presented.
3. Bioinformatics tools for scRNA-seq data analysis are thoroughly discussed.
4. Applications and further development of scRNA-seq technology are highlighted.

1. Spatial omics is transforming our understanding of the cancer ecosystem at the systemic level.

2. The integration of spatial omics and single-cell omics can fundamentally improve our understanding of tumourigenesis and cancer microenvironment.

3. Generating the spatial atlas of human cancers across multiple omics and timescales will potentially pioneer the revolution of spatiotemporal molecular medicine.

1. CAFs-derived exosomal miR-20a promotes the progression of NSCLC.
2. CAFs-derived exosomal miR-20a promotes chemoresistance of NSCLC.
3. PTEN is a target gene of miR-20a. 4. miRNA-20a suppresses the PTEN/PI3K-AKT pathway.

CircCPM is up-regulated in 5-FU resistant gastric cancer cells and tissues.

CircCPM modulates autophagy by working as a sponge of miR-21-3p, thereby up-regulating PRKAA2 expression.

CircCPM regulates gastric cancer 5-FU chemoresistance through the miR-21-3p/ PRKAA2 axis.

This article revealed that the circST3GAL6 inhibits the proliferation and metastasis of gastirc cancer through autophagy regulated by circST3GAL6/miR-300/FOXP2/MET/mTOR axis. Therefore, circST3GAL6 may serve as a prognostic marker and therapeutic target for gastric cancer.

1. CPPs are amino acid sequences that can induce uptake processes by the cells.

2. CPPs can facilitate the intracellular delivery of a variety of anti-cancer therapeutic moleculars.

3. The mechanisms of the CPPs uptake can be generally categorised into energy-dependent and direct penetration.

4. The optimisation of CPPs' defects will promote their clinical applications as drug delivery vehicles.

Midbrain samples of Parkinson's disease (PD) and control (CTR) patients were subjected to a multi-omic analysis. The expression of miRNAs, transcripts and proteins was explored, followed by data integration and functional analyses. This comprehensive assessment of PD-affected and CTR human midbrains revealed multiple molecular targets and networks that are relevant to the disease mechanism of advanced PD.