Translational Synthetic Biology

5 November 2024
6 November 2024

Edited by: Dr. Yubin Zhou, Institute of Biosciences and Technology, and Department of Translational Medical Sciences, School of Medicine, Texas A&M University, USA

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Open Access

Genetically encoded tools for in vivo G-protein-coupled receptor agonist detection at cellular resolution

Kayla E. Kroning,  Wenjing Wang, 
Genetically encoded tools for in vivo G-protein-coupled receptor agonist detection at cellular resolution

G-protein-coupled receptor (GPCR) agonist sensors utilise downstream GPCR signalling events, such as GPCR conformational changes and protein binding, to give a measurable sensor readout.

Open Access

The clinical potential of optogenetic interrogation of pathogenesis

Tianyu Terry Gao,  Teak-Jung Oh,  Kritika Mehta,  Yu-En Andrew Huang,  Tyler Camp,  Huaxun Fan,  Jeong Won Han,  Collin Michael Barnes,  Kai Zhang, 
The clinical potential of optogenetic interrogation of pathogenesis

  1. Opsin-based and opsin-free optogenetics employs light to modulate membrane conductance and protein-protein interaction.
  2. Clinical trials focus on treating blindness due to the easy delivery of light.
  3. Protein engineering improves the photophysics and biocompatibility of photoactivatable proteins.
  4. Opsin-free optogenetics expands clinical applications such as the chimeric antigen receptor (CAR) and clustered regularly interspaced short palindromic repeats (CRISPR)-based genomic engineering and transcriptional regulation.

Open Access

Boolean logic in synthetic biology and biomaterials: Towards living materials in mammalian cell therapeutics

Eric M. Bressler,  Sarah Adams,  Rong Liu,  Yolonda L. Colson,  Wilson W. Wong,  Mark W. Grinstaff, 
Boolean logic in synthetic biology and biomaterials: Towards living materials in mammalian cell therapeutics

The intersection of synthetic biology and biomaterials promises to enhance safety and efficacy in novel therapeutics. By using Boolean logic in both cellular therapy and drug delivery devices, researchers have achieved better safety and efficacy outcomes. We expect that these collaborations will continue to grow and realize the next generation of living biomaterial therapeutics.

Open Access

Development of immune-related cell-based machine learning for disease progression and prognosis of alcoholic liver disease

Xu Zhang,  Xuezhe Feng,  Qian Huai,  Cheng Zhu,  Yingyin Sun,  Xiaolei Li,  Hanren Dai,  Honglin Wang,  Hua Wang, 
Open Access

Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells

Changping Deng,  Shihui Li,  Yuping Liu,  Wen Bao,  Chengnan Xu,  Wenyun Zheng,  Meiyan Wang,  Xingyuan Ma, 
Split-Cas9-based targeted gene editing and nanobody-mediated proteolysis-targeting chimeras optogenetically coordinated regulation of Survivin to control the fate of cancer cells

  • The photoactivatable PROTACs-Like (paProtacL) target protein via nanobodies and can controllably induce degradation of POI (protein of interest) under blue light.

  • The photoactivatable Cas9 (paCas9) system can edit GOI (gene of interest) in the nucleus in a controlled manner under blue light irradiation.

  • When paProtacL is combined with the paCas9 system, it can minimize the expression of the target protein.

Open Access

RNA interference therapy for targeting ANGPTL3 and atherogenic lipoproteins: Findings and implications of a recent phase I study

Gerald F. Watts,  Dick C. Chan, 
Open Access

Optimization strategies and advances in the research and development of AAV-based gene therapy to deliver large transgenes

Valeria V. Kolesnik,  Ruslan F. Nurtdinov,  Ezekiel Sola Oloruntimehin,  Alexander V. Karabelsky,  Alexander S. Malogolovkin, 
Optimization strategies and advances in the research and development of AAV-based gene therapy to deliver large transgenes

  • AAV-based gene therapy are on increasing demand to cover the needs in the patient communities with inherited genetic disorders.
  • Single AAV vectors delivering functional gene copy are the most popular approach in RnD pipeline.
  • Multiple split vectors, inteins and trans-splicing strategy so far underperform monovector AAV-delivery.
  • Neural networks guided by protein fitness data facilitate de novo protein design to fit into AAV capsid.

Open Access

Single-cell and spatial alterations of neural cells and circuits in clinical and translational medicine

William Wang,  Xuanqi Liu,  Diane Catherine Wang, 
Open Access

Extracellular vesicles as the next-generation modulators of pharmacokinetics and pharmacodynamics of medications and their potential as adjuvant therapeutics

Jiaqi Liu,  Joel Z. Nordin,  Andrew J. McLachlan,  Wojciech Chrzanowski, 
Extracellular vesicles as the next-generation modulators of pharmacokinetics and pharmacodynamics of medications and their potential as adjuvant therapeutics

Extracellular vesicles' potential in modulating medications' pharmacokinetics and pharmacodynamics.

Open Access

Insight into prostate cancer osteolytic metastasis by RelB coordination of IL-8 and S100A4

Wenbo Sun,  Kenny Xu,  Xiao Li,  Peipei Qian,  Fan Xu,  Yanyan Zhang,  Xiumei Wang,  Zhi Xu,  Jiaji Ding,  Xinyu Xu,  Xiaowei Wei,  Qin Jiang,  Yong Xu, 
Insight into prostate cancer osteolytic metastasis by RelB coordination of IL-8 and S100A4

  • RelB activates inflammatory signalling by upregulating IL-8 and suppressing AR.
  • RelB upregulates S100A4 by cooperating with NFATC1.
  • IL-8 boosts EMT by activating Snail 1 and Twist 1, and S100A4 exacerbates osteolytic metastasis via calcium consumption.
  • RelB harnesses IL-8 and S100A4 to drive PCa osteolytic metastasis.