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ISSUE INFORMATION
REVIEW
Liquid Biopsy Series
The Bridge: Supernatant Derived From Cytological Sample Preparations
- Pages: 222-227
- First Published: 08 February 2025

Cytology-derived supernatant specimens provide a substantial source of high-quality genomic material that can be used for genotyping to guide therapeutic decision-making, as well as for other roles in cancer screening, longitudinal monitoring and disease prognostication. These novel substrates, such as supernatants from cerebrospinal fluid, sputum, urine, pleural effusion, ascitic fluid, as well as fine-needle aspiration (FNA) specimens, serve as a bridge between tissue-based testing and conventional plasma-based liquid biopsy. Cytologically derived liquid biopsy samples can not only be used in situations where the tissue sample is inadequate for genotyping, or when plasma-based liquid biopsy fails to identify an oncogenic driver alteration, but they can be used as a stand-alone complementary specimen source that can provide reliable genomic information for therapeutic decisions.
ORIGINAL ARTICLE
Fine-Needle Aspiration Biopsy of Adrenal Gland Lesions: The Roles of Image Guidance, Rapid On-Site Evaluation and Additional Tissue Sampling
- Pages: 228-235
- First Published: 29 January 2025

This study demonstrates that fine-needle aspiration (FNA) has great efficacy for evaluation of adrenal lesions, either under endoscopic ultrasound or CT guidance. Incorporation of rapid on-site evaluation into FNA procedure does not directly affect the performance of FNA biopsy but may help direct additional tissue sampling to increase diagnostic yield.
Thy1c Cytology Is a Reliable Method of Excluding Thyroid Cancer in Dominant Cystic Nodules
- Pages: 236-239
- First Published: 23 January 2025
Automated Description Generation of Cytologic Findings for Lung Cytological Images Using a Pretrained Vision Model and Dual Text Decoders: Preliminary Study
- Pages: 240-249
- First Published: 07 February 2025
Digital Cytology Combined With Artificial Intelligence Compared to Conventional Microscopy for Anal Cytology: A Preliminary Study
- Pages: 250-258
- First Published: 11 March 2025

Cytology is an accepted method of screening for anal cancer. We compared the diagnostic performance between conventional microscopy and digital cytology in a series of anal specimens. The number of ASC-US+ cases detected by both systems was similar. However, the digital cytology system detected more high-grade lesions than conventional microscopy.
This study compared the diagnostic performance between conventional microscopy (CC) and digital cytology (DC) coupled with artificial intelligence (AI) in a series of anal cytology specimens. Although the number of ASC-US+ cases detected by both systems was similar, the DC/AI system detected more high-grade lesions than CC.
Diagnostic Value of Liquid-Based Cervical Cytology Test in Endometrial Carcinoma
- Pages: 259-265
- First Published: 07 March 2025
Polymorphous Adenocarcinoma: A Cytological Enigma Unveiled Through Uncommon Locations—A Case Study
- Pages: 266-272
- First Published: 08 March 2025
CASE REPORT
Cytological Characteristics of Warthin-Like Papillary Carcinoma: A Report of Four Cases and Literature Review
- Pages: 273-277
- First Published: 25 February 2025
In this study, the author retrospectively analysed the clinical data, fine-needle aspiration (FNA) cytology features, BRAF V600E gene test results, and prognosis of four patients diagnosed with Warthin-like papillary thyroid carcinoma (WL-PTC) and reviewed the relevant literature.
Navigating Diagnostic Uncertainty in Thyroid Nodules: The Critical Role of Cytology and Histology in Oncocytic and Rare Patterned Lesions
- Pages: 278-280
- First Published: 03 February 2025
This case report highlights the diagnostic challenges posed by thyroid nodules with indeterminate cytological features, emphasising the complementary roles of fine-needle aspiration (FNA) cytology and histology. Through a detailed evaluation of a trabecular adenoma misclassified as oncocytic, the study underscores the importance of thorough histological assessment to avoid misdiagnosis and ensure accurate clinical management.
Diagnostic Insighhts Into JC Virus–Infected Glial Cells: Intraoperative Cytopathological Analysis of Dot-Shaped Inclusions Associated With PML-NBs
- Pages: 281-284
- First Published: 10 January 2025
ALK+ Anaplastic Large Cell Lymphoma Diagnosed on Paediatric Pericardial Effusion Cytology
- Pages: 285-289
- First Published: 15 March 2025
Authors describe pericardial effusion as the primary presentation of paediatric ALK+ ALCL, without a cardiac mass. This emphasises the diagnostic value of pericardiocentesis and highlights ALK+ ALCL in the differential diagnosis of malignant paediatric pericardial effusions.
Genetic Insights Into Recurrent Granular Cell Tumours in an Adolescent: The Role of Cytology and Next-Generation Sequencing in the Diagnosis of RASopathies
- Pages: 290-294
- First Published: 12 March 2025
Recurrent multiple benign granular cell tumours were observed in an adolescent with Noonan syndrome, a RASopathy linked to dysregulated RAS/MAPK signalling. This case suggests a potential association between aberrant pathway activation and tumour development, highlighting the need for evaluating associated fatal anomalies and conducting genetic studies.