Stem cells can regulate the inflammation through the secretion of cytokines. They can also prevent tissue damage and participate in tissue repair. Stem cells are considered as one of the potential therapies for COVID-19.

We report, for the first time, the expression pattern, function and regulatory mechanism of SNHG15 together with miR-18a-5p micro RNA in ovarian cancer (OC). SNHG15 could possibly serve as a promising biomarker and target in cancer treatment. Our study demonstrated that SNHG15 could promote the proliferation, migration, invasion, and chemoresistance of OC cells, through activating the protein kinase B/mammalian target of rapamycinm signaling pathway. We believe our results could support the SNHG15-based diagnosis and prognostic for patients with OC.

Low-intensity pulsed ultrasound (LIPUS) treatment decreases insulin signaling, resulting in the inhibition of adipocyte differentiation. In addition, the formation of actin stress fibers by LIPUS leads to the production of CCN2 by retention of YAP in the nucleus, and the produced CCN2 inhibits adipocyte differentiation via suppression of the gene expression of peroxisome proliferator-activated receptor-γ (PPARγ). As a result, LIPUS suppresses adipogenesis.

Our findings for the first time illustrate how RHPN1 antisense RNA 1 displayed its tumor-promotive roles in hepatocellular carcinoma (HCC) and may offer a new biomarker and a potential therapeutic target for patients with HCC.

DHPAC, a microtubule-targeted inhibitor that targets the colchicine binding site, inhibits angiogenesis and vasculogenic mimicry in non-small-cell lung cancer. In the tumor microenvironment, DHPAC can inhibit the VEGF/VEGFR2 signal transduction pathway by activating JNK. In addition, DHPAC can also inhibit vasculogenic mimicry by blocking the FAK/AKT signaling pathway.

long noncoding RNA (lncRNA) TTN-AS1 expression was significantly upregulated in breast cancer tissues and cells. The lncRNA TTN-AS1 drove the proliferation, migration, invasion, and epithelial-mesenchymal transformation of breast cancer cells via regulating miR-139-5p/zinc finger E-box binding homeobox 1 axis.

In this study, we showed the differential expressed circRNA, circular RNA, (DECs) in colorectal cancer (CRC) tissues and matched adjacent normal tissues based on deep RNA sequencing and ensured the DECs expression by combining with GSE116589 and further validated the expression of four significantly upregulated circRNAs in both of paired CRC tissues and cell lines. Moreover, we constructed the circRNA/microRNA/messenger RNA regulatory network in CRC and analyzed the potential biological functions of DECs. Furthermore, three potential bioactive compounds for the treatment of CRC were also found based on the regulatory network.

The expression of β2 adrenergic receptor (β2-AR) was increased in human periodontal lgament cells (HPDLCs) exposed to stretch loading, and its protein expression was decreased in rat periodontal ligament (PDL) tissue subjected to reduced occlusal loading. Fenoterol, a specific β2-AR agonist, led to an upregulation in the expression of PDL-related molecules and increased intracellular cyclic adenosine monophosphate levels in HPDLCs. Therefore, it was suggested that β2-AR in PDL tissues might be involved in PDL homeostasis.

FAM20C phosphorylates osteopontin (OPN) on Ser146 in the RGDSVVYGLR motif of OPN. The phosphorylation does not affect thrombin of plasmin cleavage at nearby sites, but it strongly inhibits RGD-dependent adhesion to the α_vβ₃ integrin in cells.This suggests a new mechanism by which specific phosphorylation can regulate OPN-cell interaction.

Circ_0011269 was notably downregulated in osteoporosis tissues and its expression was gradually increased during osteogenic differentiation. Network analysis constructed circ_0011269-miRNAs interaction network. Circ_0011269 sponges miR-122 to regulate RUNX2 expression and promotes osteoporosis progression.

We firstly investigated the function of microRNA-543 (miR-543) skeletal muscle myogenesis and found that miR-543 could promote differentiation and inhibit the proliferation of myoblasts. Mechanistic investigation showed that KLF6 functions as a target gene of miR-543. Moreover, the effect of KLF6 knockdown on skeletal muscle myogenesis was examined. Our study provides some clues regarding the regulatory mechanism of miR-543 on skeletal muscle myogenesis.

Our data showed that long nocoding RNA MALAT1 positively regulates death-associated protein kinase 1 expression by targeting miR-124-3p and mediates cell apoptosis and motor disorders in Parkinson's disease (PD). These results provided experimental evidence of developing potential therapeutic strategies for PD.

Exosome under oxidative stress regulates retinal pigment epithelial cell biology.

• The nutraceuticals analyzed were able to protect H₂O₂ cytotoxic effects.

• It is possible to propose the use of these compounds as a dietary supplement for the prevention of neuronal aging.

• It is possible to propose the use of these compounds as a dietary supplement for the prevention or as adjuvant to the only symptomatic treatments so far available for neurodegenerative diseases.

We demonstrate that the nuclear location of ERBB2 can results from the translation of a variant ERBB2 messenger RNA under the transcriptional control of a distal promoter, actively used in breast cancer cells. This ERBB2b isoform can directly translocate into the nucleus due to the lack of the signal peptide required for an intermediate membrane location. Our results provide new insights into the origin and function of nuclear ERBB2 where it can participate at the same time in a positive or a negative feedback autoregulatory loop, dependent on which of its promoters this bona fide transcription factor is acting.

This report explains that mtOGG-1 activity is reduced by 4HNE adduction, which increased 8OHG in the mitochondria of diabetic cardiac tissue.

Our findings show that GATA factor ELT-2 activates OGT-1 transcription via its direct binding of the ogt-1 promoter. The decreased ELT-2 expression in old age leads to the decrease of OGT-1 expression, and thereby drives natural aging of Caenorhabditis elegans. These data identify the key role of GATA factors in promoting OGT activity to modulate the C. elegans lifespan.

This study identified three key genes as biomarkers and potential therapeutic targets of endometrial cancer (EC) on the basis of integrated bioinformatics analysis. The findings will improve our comprehension of the molecular mechanisms underlying the pathogenesis and prognosis of EC.

Spt4/Spt5 is a useful target since it is likely a transcription factor that has implications for long non-coding RNA repeats related to frontotemporal dementia found in the C9orf72 disease pathology. Inhibitors for Spt4/Spt5 using peptides as a starting point for assays as a means for developing small molecules could likely lead to therapeutic development for inhibition for Spt4/Spt5 with CNS characteristics. Thus, we elucidated the specific steps of identification and modification of key interacting residues from Spt4/Spt5 complex with further effect prediction. Finally, several potent peptides with increased specificity to the Spt4/Spt5 interface were found and can be screened in cell-based and enzymatic assays for peptide screens that lead to small molecule campaigns.

• Chronic exposure to increased intraocular pressure in the microbead glaucoma model differentially modulates the retinal proteome.

• Defects in oxidative phosphorylation and glutathione metabolism are common features of both human and experimental glaucoma.

• Crystallins are the most negatively impacted proteins in the retina in glaucoma conditions.

• Human glaucoma associated classical complement pathway activation and upregulation of cholesterol transport proteins are not observed in the microbead glaucoma model.

The precise molecular mechanisms involved in the complex biology of human calcium-binding protein, spermatid-associated 1 (hCABS1) remain unclear. In this manuscript, we provide a detailed in silico analysis of relevant aspects of the structure and function of hCABS1 and postulate extracellular and intracellular roles.

This study describes a multiplexed immunofluorescence staining process aimed at exploring the expression of multiple cancer biomarkers in three-dimensional (3D) spheroid-derived formalin-fixed paraffin-embedded sections on an automated immunohistochemistry platform. We demonstrate the simultaneous detection of several key expressed biomarkers in non–small cell lung adenocarcinoma and colorectal adenocarcinoma cells on a single slide. In addition, multispectral imaging allows quantification of fluorescent signals and visualization of colocalized biomarkers. The combination of 3D spheroid technology and fluorescence multiplexing may be used to establish preclinical models for cancer drug screening and extrapolated to personalized cancer therapies using patient-derived organoids.