HEADLIGHTS:

1. DCs were divided into non-inf DCs and inf-DCs based on their expression features of IRF8 and pro-inflammatory cytokines and chemokines.

2. The percentages of inf-DC in patients from cancer were all 100%.

3. cDC2 interacted more actively with lymphocytes than cDC1 and mature-DC; inf-DC interacted more actively with lymphocytes than non-inf DC.

4. All the three inf-DC showed a general inflammation program.

EBV acts as an epigenetic driver in carcinogenesis and mainly refers to DNA methylation, RNA methylation, histone modification, chromatin accessibility, and the three-dimensional (3D) genome structure.

High glucose or oxidative stress promotes reactive oxygen species accumulation in retinal endothelial cells and induces cell senescence.

The senescent endothelial cells exhibited the secretion phenotype of senescence-related secretory phenotype and increased senescence-associated proteins.

Targeting forkhead family ‘O’ group/Sirtuins and cGAS/STING signalling pathways to inhibit endothelial cell senescence may be a new therapy for anti-angiogenesis.

A workflow for characterising mRNA target sequences unaffected by alternative splicing to ensure reproducible measurement of gene expression biomarkers.

• Liquid biopsy analyses the levels of biomarkers, which are of clinical significance present in bodily fluids like blood, serum, urine and saliva for diagnosis of cancer.
• The different biomarkers evaluated by liquid biopsy include cell-free DNA, circulating tumour DNA, non-coding RNA, proteins, exosomes and circulating tumour cells.
• The biomarkers are analysed using different sophisticated techniques such as microarray, next-generation sequencing, microfluidics, karyotyping, advanced microscopy and fluorescence in situ hybridisation.
• The biomarkers provide valuable information regarding the stage of cancer and also enlightens medical professionals about the most suitable therapy.