Cover Illustration: (Left to Right): (Image 1): Photo of a brook trout caught and released in the Little River located in Virginia, USA as part of a long-term genetic rescue experiment, one of the many management actions the dataset from Mamoozadeh et al. may be used to inform; (Image 2): Mangrove propagule floats in seawater; (Image 3): Scaphirhynchus sturgeon haploid gynogens only contain DNA from a maternal parent and only half of the genomic content of that maternal parent. The artificially produced haploids, malformed due to reduction in genome dosage, facilitate identification of paralogous sequence variants and true single nucleotide polymorphisms in paleotetraploid sturgeons. Photograph by Marlene Dodson, U.S. Geological Survey (Public Domain); (Image 4): Individual-based sampling and genomic-scale datasets lend greater resolution and power to landscape genomic approaches for answering important conservation questions; (Image 5): Two ‘Extinct in the Wild’ reptiles - the Christmas Island blue-tailed skink released to the wild on the Cocos-Keeling Islands, and the Lister's Gecko that still remain in the captive breeding program until such time they are also released to the wild. (Image 6): Male hihi (Notiomystis cincta), photographed on the island sanctuary of Tiritiri Matangi, Aotearoa New Zealand; (Image 7): NA; (Image 8): gelada; (Image 9): NA; (Image 10): Electronic illustration of anadromous Atlantic salmon during their upstream spawning migration; (Image 11): Adult migrating pouched lamprey (Geotria australis, kanakana/piharau) not displaying symptoms of lamprey reddening syndrome (LRS). (Image 12): Phylogenetic tree representing a diversity of invertebrates from museum collections utilising a high-throughput taxonomy-informed barcoding pipeline: Dopheide et al 2022; (Image 13): ddgRADer is a user friendly webtool for choosing restriction enzymes and size-selection criteria to optimise sequencing output of ddRADseq experiments; (Image 14): This picture shows one population of Chamerion angustifolium in the Kanas, Altay, Xinjiang, China. C. angustifolium is a mixed-ploidy species with three cytotypes: diploids, tetraploids and hexaploids, and it is widely across temperate Europe, Asia, and North America. A 100 GiB single nucleotide polymorphism genotype dataset of C. angustifolium was used in the benchmark test of VCFPOP software on a desktop computer. VCFPOP can perform a variety of population genetic analyses for haploids, diploids, autopolyploids and aneuploids, and supports a maximum ploidy level of 10; (Image 15): Spotted hyena in Ngorongoro Crater defecates. Non-invasive sampling of genetic material such as that contained in mucus covering faeces is a critical tool for population genetic studies; (Image 16): The haplotype network of fifty SARS-COV-2 genomes constructed with fastHaN; (Image 17): Sexually monomorphic species are challenging to study. Sex-linked genetic loci, which are easily identified using our R functions, can be used to sex individuals. Sex-linked loci are also important to filter out before assessing population structure, genetic diversity and parentage. Endangered helmeted honeyeaters, shown here just after mating, are sexually monomorphic, but their sex was easily assigned using our R functions; (Image 18): Minimally invasive mucus swabbing of juvenile Chinook salmon to collect DNA for SHERLOCK assay run identification; (Image 19): Dissection of largemouth bass stomach preserved in ethanol. DNA from Chinook salmon prey was extracted following dissection; (Image 20): Leavenworthia exigua in fruit in the field; (Image 21): A brook trout (Salvelinus fontinalis) swims in a cold Pennsylvania stream. Landscape transcriptomics has important implications for future management of this iconic species.

Photo Credit: (Left to Right): (Image 1): Sam Rosenbaum; (Image 2): Gustavo Maruyama Mori; (Image 3): Marlene Dodson, U.S. Geological Survey (Public Domain); (Image 4): E. Anne Chambers (illustrator); (Image 5): BTS - Parks Australia; Gecko - Jason Turl; (Image 6): Charlotte Johnson; (Image 7): Andrew Digby; (Image 8): Noah Snyder-Mackler; (Image 9): Alyssa with WOMBO; (Image 10): Caroline Côté (Image 11): Jonah Yick; (Image 12): Photos from the New Zealand Arthropod Collection provided by Darren Ward and Thomas Buckley. Phylogenetic tree compiled by Andrew Dopheide and art composition by Andrew Dopheide, Manpreet Dhami & Cissy Pan; (Image 13): Felix Glinka; (Image 14): Tongcheng Wang; (Image 15): Oliver Honer; (Image 16): Xialong Zhang; (Image 17): Merrilyn Serong; (Image 18): Melinda Baerwald; (Image 19): Wes Larson; (Image 20): Brigette Williams; (Image 21): Justin Waraniak.