Nanofibers added with citral: Characterization and their application to postharvest control of Fusarium pseudocircinatum in bananas
Maricarmen Iñiguez-Moreno
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Universidad Politécnica Del Estado de Nayarit, Carretera Tepic-Aguamilpa, Tepic, Nayarit, Mexico
Search for more papers by this authorCorresponding Author
Montserrat Calderón-Santoyo
Correspondence
Juan Arturo Ragazzo-Sánchez and Montserrat Calderón-Santoyo, Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Av. Tecnologico, Tepic, Nayarit, 63175, Mexico.
Email: [email protected]; [email protected]
Search for more papers by this authorJulio César Barros-Castillo
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Search for more papers by this authorDulce María Miss-Zacarías
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Search for more papers by this authorJosé Alfredo Díaz
Departamento de Química Física e Inorgánica, Escuela de Química, Facultad de Ciencias Naturales y Matemáticas, Universidad de El Salvador, San Salvador, El Salvador
Search for more papers by this authorCorresponding Author
Juan Arturo Ragazzo-Sánchez
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Correspondence
Juan Arturo Ragazzo-Sánchez and Montserrat Calderón-Santoyo, Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Av. Tecnologico, Tepic, Nayarit, 63175, Mexico.
Email: [email protected]; [email protected]
Search for more papers by this authorMaricarmen Iñiguez-Moreno
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Universidad Politécnica Del Estado de Nayarit, Carretera Tepic-Aguamilpa, Tepic, Nayarit, Mexico
Search for more papers by this authorCorresponding Author
Montserrat Calderón-Santoyo
Correspondence
Juan Arturo Ragazzo-Sánchez and Montserrat Calderón-Santoyo, Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Av. Tecnologico, Tepic, Nayarit, 63175, Mexico.
Email: [email protected]; [email protected]
Search for more papers by this authorJulio César Barros-Castillo
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Search for more papers by this authorDulce María Miss-Zacarías
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Search for more papers by this authorJosé Alfredo Díaz
Departamento de Química Física e Inorgánica, Escuela de Química, Facultad de Ciencias Naturales y Matemáticas, Universidad de El Salvador, San Salvador, El Salvador
Search for more papers by this authorCorresponding Author
Juan Arturo Ragazzo-Sánchez
Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Tepic, Nayarit, México
Correspondence
Juan Arturo Ragazzo-Sánchez and Montserrat Calderón-Santoyo, Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México / Instituto Tecnológico de Tepic, Av. Tecnologico, Tepic, Nayarit, 63175, Mexico.
Email: [email protected]; [email protected]
Search for more papers by this authorAbstract
The use of essential oils (EOs) in the food industry is limited due to their susceptibility to environmental conditions. Therefore, this study aimed to encapsulate citral into nanofibers of pullulan and whey protein concentrate (WPC) to control Fusarium crown rot in bananas. Nanofibers without beads, able to prevent thermal and photodegradation of citral, were obtained by the electrospinning process. Besides, citral produced a significant change in the infrared spectra of nanofibers, reaching a loading efficiency of the process estimated by thermogravimetry was 19%. Finally, the nanofibers added with citral reduced 66% of the infection produced by Fusarium pseudocircinatum in bananas. Therefore, the nanofibers of pullulan/WPC are an alternative to encapsulate citral and protect it from environmental conditions, providing an eco-friendly coating that contributes to the management of Fusarium crown root in bananas.
Novelty impact statement
The use of whey protein concentrate and pullulan allowed to obtain nanofibers able to provide thermal and photoprotection to citral. Citral addition did not affect the nanofiber morphology and slight changes in the infrared spectra were observed. Nanofibers with the essential oil reduced 66% of the development of Fusarium crown root in bananas, being an alternative to postharvest biocontrol in these fruits at 25°C.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
REFERENCES
- Alghuthaymi, M., Alshehri, W. A., Al-Maary, K. S., Bahkali, N. A., AlKahtani, M. D. F., Alarfaj, A. A., Alnadhari, S., & Ameen, F. (2020). Mycotoxigenicity of Fusarium isolated from banana fruits: Combining phytopathological assays with toxin concentrations. Journal of King Saud University - Science, 32, 1482–1485. https://doi.org/10.1016/j.jksus.2019.12.001
- Aytac, Z., Celebioglu, A., Yildiz, Z. I., & Uyar, T. (2018). Efficient encapsulation of citral in fast-dissolving polymer-free electrospun nanofibers of cyclodextrin inclusion complexes: High thermal stability, longer shelf-life, and enhanced water solubility of citral. Nanomaterials, 8, 793. https://doi.org/10.3390/nano8100793
- Beikzadeh, S., Akbarinejad, A., Swift, S., Perera, J., Kilmartin, P. A., & Travas-Sejdic, J. (2020). Cellulose acetate electrospun nanofibers encapsulating Lemon Myrtle essential oil as active agent with potent and sustainable antimicrobial activity. Reactive and Functional Polymers, 157, 104769. https://doi.org/10.1016/j.reactfunctpolym.2020.104769
- Calderón-Santoyo, M., Iñiguez-Moreno, M., Barros-Castillo, J. C., Miss-Zacarías, D. M., Díaz, J. A., & Ragazzo-Sánchez, J. A. (2022a). Microencapsulation of citral with Arabic gum and sodium alginate for the control of Fusarium pseudocircinatum in bananas. Iranian Polymer Journal, 31, 665–676. https://doi.org/10.1007/s13726-022-01033-z
- Calderón-Santoyo, M., Iñiguez-Moreno, M., & Ragazzo-sánchez, J. A. (2022b). Microencapsulation of citral and its antifungal activity into pectin films. Biointerface Research in Applied Chemistry, 12, 7488–7502.
- Cheng, K. C., Demirci, A., & Catchmark, J. M. (2011). Pullulan: Biosynthesis, production, and applications. Applied Microbiology and Biotechnology, 92, 29–44. https://doi.org/10.1007/s00253-011-3477-y
- Cheng, M., Wang, J., Zhang, R., Kong, R., Lu, W., & Wang, X. (2019). Characterization and application of the microencapsulated carvacrol/sodium alginate films as food packaging materials. International Journal of Biological Macromolecules, 141, 259–267. https://doi.org/10.1016/j.ijbiomac.2019.08.215
- Chu, Y., Xu, T., Gao, C. C., Liu, X., Zhang, N., Feng, X., Liu, X., Shen, X., & Tang, X. (2019). Evaluations of physicochemical and biological properties of pullulan-based films incorporated with cinnamon essential oil and Tween 80. International Journal of Biological Macromolecules, 122, 388–394. https://doi.org/10.1016/j.ijbiomac.2018.10.194
- Contarino, R., Brighina, S., Fallico, B., Cirvilleri, G., Parafati, L., & Restuccia, C. (2019). Volatile organic compounds (VOCs) produced by biocontrol yeasts. Food Microbiology, 82, 70–74. https://doi.org/10.1016/j.fm.2019.01.008
- Cruz-Salas, C. N., Prieto, C., Calderón-Santoyo, M., Lagarón, J. M., & Ragazzo-Sánchez, J. A. (2019). Micro-and nanostructures of agave fructans to stabilize compounds of high biological value via electrohydrodynamic processing. Nanomaterials, 9, 1659. https://doi.org/10.3390/nano9121659
- Djordjevic, D., Cercaci, L., Alamed, J., McClements, D. J., & Decker, E. A. (2008). Stability of citral in protein- and gum arabic-stabilized oil-in-water emulsions. Food Chemistry, 106, 698–705. https://doi.org/10.1016/j.foodchem.2007.06.033
- Drosou, C., Krokida, M., & Biliaderis, C. G. (2018). Composite pullulan-whey protein nanofibers made by electrospinning: Impact of process parameters on fiber morphology and physical properties. Food Hydrocolloids, 77, 726–735. https://doi.org/10.1016/j.foodhyd.2017.11.014
- Farris, S., Unalan, I. U., Introzzi, L., Fuentes-Alventosa, J. M., & Cozzolino, C. A. (2014). Pullulan-based films and coatings for food packaging: Present applications, emerging opportunities, and future challenges. Journal of Applied Polymer Science, 131, 40539. https://doi.org/10.1002/app.40539
- Gundewadi, G., Rudra, S. G., Gogoi, R., Banerjee, T., Singh, S. K., Dhakate, S., & Gupta, A. (2021). Electrospun essential oil encapsulated nanofibers for the management of anthracnose disease in Sapota. Industrial Crops and Products, 170, 113727. https://doi.org/10.1016/j.indcrop.2021.113727
- Iñiguez-Moreno, M., Ragazzo-Sánchez, J. A., Barros-Castillo, J. C., Sandoval-Contreras, T., & Calderón-Santoyo, M. (2020). Sodium alginate coatings added with Meyerozyma caribbica: Postharvest biocontrol of Colletotrichum gloeosporioides in avocado (Persea americana Mill. cv. Hass). Postharvest Biology and Technology, 163, 111123. https://doi.org/10.1016/j.postharvbio.2020.111123
- Iñiguez-Moreno, M., Ragazzo-Sánchez, J. A., Barros-Castillo, J. C., Solís-Pacheco, J. R., & Calderón-Santoyo, M. (2021). Characterization of sodium alginate coatings with Meyerozyma caribbica and impact on quality properties of avocado fruit. LWT - Food Science and Technology, 152, 112346. https://doi.org/10.1016/j.lwt.2021.112346
- Kesici Güler, H., Cengiz Çallıoğlu, F., & Sesli Çetin, E. (2019). Antibacterial PVP/cinnamon essential oil nanofibers by emulsion electrospinning. Journal of the Textile Institute, 110, 302–310. https://doi.org/10.1080/00405000.2018.1477237
- Ma, H., Zhao, Y., Lu, Z., Xing, R., Yao, X., Jin, Z., Wang, Y., & Yu, F. (2020). Citral-loaded chitosan/carboxymethyl cellulose copolymer hydrogel microspheres with improved antimicrobial effects for plant protection. International Journal of Biological Macromolecules, 164, 986–993. https://doi.org/10.1016/j.ijbiomac.2020.07.164
- Madrigal, L., Sandoval, A. J., & Müller, A. J. (2011). Effects of corn oil on glass transition temperatures of cassava starch. Carbohydrate Polymers, 85, 875–884. https://doi.org/10.1016/j.carbpol.2011.04.013
- Miss-Zacarías, D. M., Iñiguez-Moreno, M., Calderón-Santoyo, M., & Ragazzo-Sánchez, J. A. (2022). Optimization of ultrasound-assisted microemulsions of citral using biopolymers: Characterization and antifungal activity. Journal of Dispersion Science and Technology, 43, 1373–1382. https://doi.org/10.1080/01932691.2020.1857264
- Nascimento, J. I. G., Stamford, T. C. M., Melo, N. F. C. B., Nunes, I. d. S., Lima, M. A. B., Pintado, M. M. E., Montenegro, T., Pereira, N., & Stamford, T. L. M. (2020). Chitosan–citric acid edible coating to control Colletotrichum gloeosporioides and maintain quality parameters of fresh-cut guava. International Journal of Biological Macromolecules, 163, 1127–1135. https://doi.org/10.1016/j.ijbiomac.2020.07.067
- Poudel, D., Swilley-Sanchez, S., O'keefe, S., Matson, J., Long, T., & Fernández-Fraguas, C. (2020). Novel electrospun pullulan fibers incorporating hydroxypropyl-β-cyclodextrin: Morphology and relation with rheological properties. Polymers, 12, 2558.
- Saha, C., Ahamed, M., Hosen, M., Nandi, R., & Kabir, M. (2021). Post-harvest losses of banana in fresh produce marketing chain in Tangail District of Bangladesh. Journal of Bangladesh Agricultural University, 19, 389. https://doi.org/10.5455/jbau.74902
10.5455/JBAU.74902 Google Scholar
- Szekalska, M., Wróblewska, M., Trofimiuk, M., Basa, A., & Winnicka, K. (2019). Alginate oligosaccharides affect mechanical properties and antifungal activity of alginate buccal films with posaconazole. Marine Drugs, 17, 692. https://doi.org/10.3390/md17120692
- Tang, Y., Zhou, Y., Lan, X., Huang, D., Luo, T., Ji, J., Mafang, Z., Miao, X., Wang, H., & Wang, W. (2019). Electrospun gelatin nanofibers encapsulated with peppermint and chamomile essential oils as potential edible packaging. Journal of Agricultural and Food Chemistry, 67, 2227–2234. https://doi.org/10.1021/acs.jafc.8b06226
- Tavassoli-Kafrani, E., Goli, S. A. H., & Fathi, M. (2018). Encapsulation of orange essential Oil using cross-linked electrospun gelatin nanofibers. Food and Bioprocess Technology, 11, 427–434. https://doi.org/10.1007/s11947-017-2026-9
- Tian, H., Lu, Z., Li, D., & Hu, J. (2018). Preparation and characterization of citral-loaded solid lipid nanoparticles. Food Chemistry, 248, 78–85. https://doi.org/10.1016/j.foodchem.2017.11.091
- Tian, H., Yang, X., Ho, C. T., Huang, Q., & Song, S. (2013). Development of a solid phase microextraction protocol for the GC-MS determination of volatile off-flavour compounds from citral degradation in oil-in-water emulsions. Food Chemistry, 141, 131–138. https://doi.org/10.1016/j.foodchem.2013.02.112
- Unalan, I., Slavik, B., Buettner, A., Goldmann, W. H., Frank, G., & Boccaccini, A. R. (2019). Physical and antibacterial properties of peppermint essential oil loaded Poly (ε-caprolactone) (PCL) electrospun fiber mats for wound healing. Frontiers in Bioengineering and Biotechnology, 7, 346. https://doi.org/10.3389/fbioe.2019.00346
- Xue, J., Wu, T., Dai, Y., & Xia, Y. (2019). Electrospinning and electrospun nanofibers: Methods, materials, and applications. Chemical Reviews, 119, 5298–5415. https://doi.org/10.1021/acs.chemrev.8b00593.Electrospinning
- Zhang, M., Sun, H., Liu, Y., Wang, Y., Piao, C., Cai, D., Wang, Y., & Liu, J. (2020). Effect of pullulan concentration and pH on the interactions between whey protein concentrate and pullulan during gelation. Journal of the Science of Food and Agriculture, 101, 659–665. https://doi.org/10.1002/jsfa.10678
- Zhang, Z., Mo, Z., Zhang, X., Wang, J., Li, J., Shi, H., Pang, W., & Lin, Z. (2022). The antifungal activity and action mechanism of lemongrass (Cymbopogon flexuosus) essential oil against Fusarium avenaceum. Journal of Essential Oil Bearing Plants, 25, 1–12.
- Zheng, S., Jing, G., Wang, X., Ouyang, Q., Jia, L., & Tao, N. (2015). Citral exerts its antifungal activity against Penicillium digitatum by affecting the mitochondrial morphology and function. Food Chemistry, 178, 76–81. https://doi.org/10.1016/j.foodchem.2015.01.077
