Journal of Food Processing and Preservation

ORIGINAL ARTICLE

Investigation on the effects of drying methods on the structure and antioxidant activity of Tremella fuciformis polysaccharides using asymmetrical flow field-flow fractionation

Yuxi Guo

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

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Yushan Sun,

Yushan Sun

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

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Xue Chen,

Xue Chen

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

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Hong Ye,

Corresponding Author

Hong Ye

[email protected]

Health Science Center, Hebei University, Baoding, China

Correspondence

Hong Ye, Health Science Center, Hebei University, Baoding 071000, China.

Email: [email protected]

Haiyang Dou, Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding 071000, China.

Email: [email protected]

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Haiyang Dou,

Corresponding Author

Haiyang Dou

[email protected]

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

Affiliated Hospital of Hebei University, Baoding, China

Correspondence

Hong Ye, Health Science Center, Hebei University, Baoding 071000, China.

Email: [email protected]

Haiyang Dou, Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding 071000, China.

Email: [email protected]

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Yuxi Guo,

Yuxi Guo

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

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Yushan Sun,

Yushan Sun

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

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Xue Chen,

Xue Chen

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

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Hong Ye,

Corresponding Author

Hong Ye

[email protected]

Health Science Center, Hebei University, Baoding, China

Correspondence

Hong Ye, Health Science Center, Hebei University, Baoding 071000, China.

Email: [email protected]

Haiyang Dou, Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding 071000, China.

Email: [email protected]

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Haiyang Dou,

Corresponding Author

Haiyang Dou

[email protected]

Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding, China

Affiliated Hospital of Hebei University, Baoding, China

Correspondence

Hong Ye, Health Science Center, Hebei University, Baoding 071000, China.

Email: [email protected]

Haiyang Dou, Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Disease of Hebei Province, School of Basic Medical Sciences, Hebei University, Baoding 071000, China.

Email: [email protected]

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First published: 13 September 2022

https://doi.org/10.1111/jfpp.17165

Citations: 3

Yuxi Guo, Yushan Sun, and Xue Chen contributed equally to this work.

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Abstract

Polysaccharide is an important bioactive component in Tremella fuciformis (T. fuciformis). In this study, the effects of three drying methods (i.e., vacuum freeze-drying, microwave drying, and hot air drying) on the structure in terms of molecular weight (M_w) and radius of gyration, and conformation of T. fuciformis polysaccharide (TFP) were investigated by asymmetrical flow field-flow fractionation coupled online with multiangle light scattering and differential refractive index detectors. The physicochemical properties of TFPs were studied by scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and differential scanning calorimetry. Furthermore, the structure-antioxidation relationship of TFPs was investigated. Results revealed that three drying methods did not significantly affect functional groups, crystal structure, and thermal property of TFPs but led to some differences in the morphology and M_w of TFPs. The TFP obtained by microwave drying had the highest DPPH radical scavenging activity, which could be attributed to low M_w.

Novelty impact statement

The three drying methods (i.e., vacuum freeze-drying, microwave drying, and hot air drying) studied in this work did not affect the functional groups and thermal properties of T. fuciformis polysaccharides (TFPs), but significantly affected the morphology of TFP granules and the M_w distribution of TFP molecules. TFP-M had the smallest M_w (1.36 ± 0.10 × 10⁷ g/mol), which could be beneficial to its DPPH radical scavenging activity.

CONFLICT OF INTEREST

The authors declare no conflicts of interest.

Open Research

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available from the corresponding author upon reasonable request.

REFERENCES

Andersson, M., Wittgren, B., & Wahlund, K. G. (2003). Accuracy in multiangle light scattering measurements for molar mass and radius estimations. Model calculations and experiments. Analytical Chemistry, 75, 4279–4291. https://doi.org/10.1021/ac030128
10.1021/ac030128+
CAS PubMed Web of Science® Google Scholar
Chen, G. J., Li, C. F., Wang, S. S., Mei, X. F., Zhang, H. X., & Kan, J. Q. (2019). Characterization of physicochemical properties and antioxidant activity of polysaccharides from shoot residues of bamboo (Chimonobambusa quadrangularis): Effect of drying procedures. Food Chemistry, 292, 281–293. https://doi.org/10.1016/j.foodchem.2019.04.060
10.1016/j.foodchem.2019.04.060
CAS PubMed Web of Science® Google Scholar
Chen, X., Tian, Y., Zhang, J., Li, Y., Zhang, W., Zhang, J., Dou, Y., & Dou, H. (2022). Study on effects of preparation method on the structure and antioxidant activity of protein-tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation. Food Chemistry, 384, 132619. https://doi.org/10.1016/j.foodchem.2022.132619
10.1016/j.foodchem.2022.132619
CAS PubMed Web of Science® Google Scholar
Chen, X., Zhang, W. H., Dou, Y. W., Song, T. G., Shen, S. G., & Dou, H. Y. (2021). Applications of asymmetrical flow field-flow fractionation for separation and characterization of polysaccharides: A review. Journal of Chromatography A, 1635, 461726. https://doi.org/10.1016/j.chroma.2020.461726
10.1016/j.chroma.2020.461726
CAS PubMed Web of Science® Google Scholar
Dai, S. S., Zhang, W. H., Dou, Y. W., Liu, H., Chen, X., Shi, J., & Dou, H. (2021). Towards a better understanding of the relationships between the structure and antitumor activity of Gastrodia elata polysaccharides by asymmetrical flow field-flow fractionation. Food Research International, 149, 110673. https://doi.org/10.1016/j.foodres.2021.110673
10.1016/j.foodres.2021.110673
CAS PubMed Web of Science® Google Scholar
Fan, L., Li, J., Deng, K., & Ai, L. J. (2012). Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum. Carbohydrate Polymers, 87, 1849–1854. https://doi.org/10.1016/j.carbpol.2011.10.018
10.1016/j.carbpol.2011.10.018
CAS Web of Science® Google Scholar
Fernando, I., Sanjeewa, K., Samarakoon, K. W., Lee, W. W., & Jeon, Y. J. (2017). FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae. Algae, 32, 75–86. https://doi.org/10.4490/algae.2017.32.12.1
10.4490/algae.2017.32.12.1
CAS Web of Science® Google Scholar
Fuentes, C., Saari, H., Choi, J., Lee, S., Sjöö, M., Wahlgren, M., & Nilsson, L. (2019). Characterization of non-solvent precipitated starch using asymmetrical flow field-flow fractionation coupled with multiple detectors. Carbohydrate Polymers, 206, 21–28. https://doi.org/10.1016/j.carbpol.2018.10.100
10.1016/j.carbpol.2018.10.100
CAS PubMed Web of Science® Google Scholar
Guo, P. P., Li, Y. Q., An, J. X., Shen, S. G., & Dou, H. Y. (2019). Study on structure-function of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors: A review. Carbohydrate Polymers, 226, 115330. https://doi.org/10.1016/j.carbpol.2019.115330
10.1016/j.carbpol.2019.115330
CAS PubMed Web of Science® Google Scholar
Huang, Z. P., Huang, Y. N., Li, X. B., & Zhang, L. N. (2009). Molecular mass and chain conformations of Rhizoma Panacis Japonici polysaccharides. Carbohydrate Polymers, 78, 596–601. https://doi.org/10.1016/j.carbpol.2009.05.027
10.1016/j.carbpol.2009.05.027
CAS Web of Science® Google Scholar
Jia, X., Dong, L., Yang, Y., Yuan, S., Zhang, Z., & Yuan, M. (2013). Preliminary structural characterization and antioxidant activities of polysaccharides extracted from hawk tea (Litsea coreana var. lanuginosa). Carbohydrate Polymers, 95, 195–199. https://doi.org/10.1016/j.carbpol.2013.03.005
10.1016/j.carbpol.2013.03.005
CAS PubMed Web of Science® Google Scholar
Li, M., Ma, F., Li, R., Ren, G., & Wu, J. (2020). Degradation of tremella fuciformis polysaccharide by a combined ultrasound and hydrogen peroxide treatment: Process parameters, structural characteristics, and antioxidant activities. International Journal of Biological Macromolecules, 160, 979–990. https://doi.org/10.1016/j.ijbiomac.2020.05.216
10.1016/j.ijbiomac.2020.05.216
CAS PubMed Web of Science® Google Scholar
Li, Y. B., Chen, J. C., Lai, P. F., Tang, B. S., & Wu, L. (2020). Influence of drying methods on the physicochemical properties and nutritional composition of instant tremella fuciformis. Food Science and Technology, 40, 741–748. https://doi.org/10.1590/fst.20519
10.1590/fst.20519
Web of Science® Google Scholar
Lin, C. P., & Tsai, S. Y. (2019). Differences in the moisture capacity and thermal stability of tremella fuciformis polysaccharides obtained by various drying processes. Molecules, 24, 2856. https://doi.org/10.3390/molecules24152856
10.3390/molecules24152856
CAS PubMed Web of Science® Google Scholar
Liu, J., Meng, C. G., Yan, Y. H., Shan, Y. N., Kan, J., & Jin, C. H. (2016). Structure, physical property and antioxidant activity of catechin grafted tremella fuciformis polysaccharide. International Journal of Biological Macromolecules, 82, 719–724. https://doi.org/10.1016/j.ijbiomac.2015.11.027
10.1016/j.ijbiomac.2015.11.027
CAS PubMed Web of Science® Google Scholar
Ma, Q. Q., Santhanam, R. K., Xue, Z. H., Guo, Q. W., Gao, X. D., & Chen, H. X. (2018). Effect of different drying methods on the physicochemical properties and antioxidant activities of mulberry leaves polysaccharides. International Journal of Biological Macromolecules, 119, 1137–1143. https://doi.org/10.1016/j.ijbiomac.2018.08.023
10.1016/j.ijbiomac.2018.08.023
CAS PubMed Web of Science® Google Scholar
Wang, Q. F., Li, S., Han, X., Ni, Y. Y., Zhao, D. D., & Hao, J. X. (2019). Quality evaluation and drying kinetics of shitake mushrooms dried by hot air, infrared and intermittent microwave-assisted drying methods. LWT-Food Science and Technology, 107, 236–242. https://doi.org/10.1016/j.lwt.2019.03.020
10.1016/j.lwt.2019.03.020
CAS Web of Science® Google Scholar
Wang, X., Xu, X., & Zhang, L. (2008). Thermally induced conformation transition of triple-helical lentinan in NaCl aqueous solution. The Journal of Physical Chemistry B, 112, 10343–10351. https://doi.org/10.1021/jp802174v
10.1021/jp802174v
CAS PubMed Web of Science® Google Scholar
Winter, W. T. (1982). Polysaccharide structure by x-ray fiber diffraction. Methods in Enzymology, 83, 87–104. https://doi.org/10.1016/S0091-679X(08)61416-8
10.1016/0076-6879(82)83007-3
CAS PubMed Web of Science® Google Scholar
Wittgren, B., Wahlund, K. G., Dérand, H., & Wesslén, B. (1996). Aggregation behavior of an amphiphilic graft copolymer in aqueous medium studied by asymmetrical flow field-flow fractionation. Macromolecules, 29(1), 268–276. https://doi.org/10.1021/ma950837s
10.1021/ma950837s
CAS Web of Science® Google Scholar
Wu, D. T., Deng, Y., Zhao, J., & Li, S. P. (2017). Molecular characterization of branched polysaccharides from tremella fuciformis by asymmetrical flow field-flow fractionation and size exclusion chromatography. Journal of Separation Science, 107, 236–242. https://doi.org/10.1002/jssc.201700615
10.1002/jssc.201700615
Web of Science® Google Scholar
Wu, Y. J., Wei, Z. X., Zhang, F. M., Linhardt, R. J., Sun, P. L., & Zhang, A. Q. (2019). Structure, bioactivities and applications of the polysaccharides from tremella fuciformis mushroom: A review. International Journal of Biological Macromolecules, 121, 1005–1010. https://doi.org/10.1016/j.ijbiomac.2018.10.117
10.1016/j.ijbiomac.2018.10.117
CAS PubMed Web of Science® Google Scholar
Xiao, H., Li, H., Wen, Y., Jiang, D., Zhu, S., He, X., Xiong, Q., Gao, J., Hou, S., Huang, S., He, L., & Liang, J. (2021). Tremella fuciformis polysaccharides ameliorated ulcerative colitis via inhibiting inflammation and enhancing intestinal epithelial barrier function. International Journal of Biological Macromolecules, 180, 633–642. https://doi.org/10.1016/j.ijbiomac.2021.03.083
10.1016/j.ijbiomac.2021.03.083
CAS PubMed Web of Science® Google Scholar
Xu, X. Q., Chen, A. J., Ge, X. Y., Li, S., Zhang, T., & Xu, H. (2020). Chain conformation and physicochemical properties of polysaccharide (glucuronoxylomannan) from fruit bodies of tremella fuciformis. Carbohydrate Polymers, 245, 116354. https://doi.org/10.1016/j.carbpol.2020.116354
10.1016/j.carbpol.2020.116354
CAS PubMed Web of Science® Google Scholar
Yang, D. D., Liu, Y., & Zhang, L. J. (2019). Tremella polysaccharide: The molecular mechanisms of its drug action. Progress in Molecular Biology and Translational Science, 163, 383–421. https://doi.org/10.1016/bs.pmbts.2019.03.002
10.1016/bs.pmbts.2019.03.002
CAS PubMed Web of Science® Google Scholar
Yuan, Q., He, Y., Xiang, P. Y., Huang, Y. J., Cao, Z. W., Shen, S. W., Zhao, L., Zhang, Q., Qin, W., & Wu, D. T. (2020). Influences of different drying methods on the structural characteristics and multiple bioactivities of polysaccharides from okra (Abelmoschus esculentus). International Journal of Biological Macromolecules, 147, 1053–1063. https://doi.org/10.1016/j.ijbiomac.2019.10.073
10.1016/j.ijbiomac.2019.10.073
CAS PubMed Web of Science® Google Scholar

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Investigation on the effects of drying methods on the structure and antioxidant activity of Tremella fuciformis polysaccharides using asymmetrical flow field-flow fractionation

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Investigation on the effects of drying methods on the structure and antioxidant activity of Tremella fuciformis polysaccharides using asymmetrical flow field-flow fractionation

Abstract

Novelty impact statement

CONFLICT OF INTEREST

Open Research

DATA AVAILABILITY STATEMENT

REFERENCES

Citing Literature

References

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