Polymeric nanoparticles loaded with Baccharis dracunculifolia DC essential oil: Preparation, characterization, and antibacterial activity in milk
Palmira Penina Raúl Timbe
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Search for more papers by this authorAmanda de Souza da Motta
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Search for more papers by this authorHenrique Ataíde Isaía
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Search for more papers by this authorCorresponding Author
Adriano Brandelli
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Correspondence
Adriano Brandelli, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, ICTA-UFRGS, Av. Bento Gonçalves 9500, Porto Alegre 91501-970, Brazil.
Email: [email protected]
Search for more papers by this authorPalmira Penina Raúl Timbe
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Search for more papers by this authorAmanda de Souza da Motta
Departamento de Microbiologia, Imunologia e Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Search for more papers by this authorHenrique Ataíde Isaía
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Search for more papers by this authorCorresponding Author
Adriano Brandelli
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
Correspondence
Adriano Brandelli, Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, Universidade Federal do Rio Grande do Sul, ICTA-UFRGS, Av. Bento Gonçalves 9500, Porto Alegre 91501-970, Brazil.
Email: [email protected]
Search for more papers by this authorAbstract
Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N-EO) was developed by the nanoprecipitation method. N-EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N-EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, and Salmonella Enteritidis. No viable cell counts were detected for all strains tested up to 4 hr incubation with EO. The same effect was achieved at 24 hr by the N-EO, suggesting a controlled release of encapsulated EO. The antimicrobial activity was also tested against L. monocytogenes in milk. Both EO and N-EO lack antibacterial effect in whole milk, while a delayed lag phase was observed in skimmed milk. The N-EO caused no significant lysis of erythrocytes suggesting that it can be safe to eukaryotic cells.
Practical applications
The results of this work suggest that Eudragit nanoparticles can be suitable carriers for essential oils, maintaining their antimicrobial activities. Nanoparticle encapsulation may be useful for food applications by hiding the intense flavor of EO and providing a controlled release of bioactive components. The reduced activity in milk suggests the interaction between the nanostructures and EO with food components, which merits additional studies.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
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