Antimicrobial peptides from the edible insect Musca domestica and their preservation effect on chilled pork
Corresponding Author
Xiangli Dang
School of Plant Protection, Anhui Agricultural University, Hefei, China
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Correspondence
Xiangli Dang, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Email: [email protected]
Search for more papers by this authorXiaoxia Zheng
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Search for more papers by this authorYansheng Wang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Search for more papers by this authorLifang Wang
School of Horticulture, Anhui Agricultural University, Hefei, China
Search for more papers by this authorLiang Ye
School of Plant Protection, Anhui Agricultural University, Hefei, China
Search for more papers by this authorJunqi Jiang
School of Plant Protection, Anhui Agricultural University, Hefei, China
Search for more papers by this authorCorresponding Author
Xiangli Dang
School of Plant Protection, Anhui Agricultural University, Hefei, China
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Correspondence
Xiangli Dang, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China.
Email: [email protected]
Search for more papers by this authorXiaoxia Zheng
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Search for more papers by this authorYansheng Wang
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
Search for more papers by this authorLifang Wang
School of Horticulture, Anhui Agricultural University, Hefei, China
Search for more papers by this authorLiang Ye
School of Plant Protection, Anhui Agricultural University, Hefei, China
Search for more papers by this authorJunqi Jiang
School of Plant Protection, Anhui Agricultural University, Hefei, China
Search for more papers by this authorAbstract
Antimicrobial compounds from natural sources are a promising alternative to address the consumer demand of food without chemical additives. In this study, antimicrobial peptides (AMPs) from infected Musca domestica pupae (Md-AMPs) bred under clean conditions were isolated and prepared on a large scale. In these Md-AMPs, seven AMPs families were identified by liquid chromatography–tandem mass spectrometry. Md-AMPs at concentrations from 0.4 to 0.8 mg/ml exhibited antibacterial activity and showed negligible hemolytic activity against human erythrocytes. Md-AMPs increased bacterial membrane permeability of four tested strains, resulting in the leakage of phosphorus-containing materials and Ca2+. Md-AMPs bound plasmid DNA and inhibited its migration in a concentration-dependent manner. Treatment with Md-AMPs alone or in combination with nisin, and ethylenediaminetetraacetic acid was efficient in inhibiting bacterial growth in chilled pork and extended the shelf life up to 6 days.
Practical applications
Insects have been widely used as foods and many biofunctional components are found in insects. In this work, we isolated and prepared AMPs from M. domestica, and its antibacterial activity, hemolytic activity, and mechanism of action were investigated. Trials in real food products showed that Md-AMPs alone or a combination of three natural antimicrobials could remarkably inhibit bacterial growth in chilled pork. These results suggest that Md-AMPs can be used as food preservatives.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
Supporting Information
| Filename | Description |
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| jfpp14369-sup-0001-FigS1.docWord document, 2.3 MB | |
| jfpp14369-sup-0002-FigS2.docxWord document, 61.6 KB | |
| jfpp14369-sup-0003-TableS1.docxWord document, 18 KB |
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