Volume 42, Issue 1 e13330
Original Article

Evaluation of the robustness of the enzymatic hydrolysis in batch and continuous mode by a central composite design

N. Abdi

N. Abdi

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

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O. Kitous

O. Kitous

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

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H. Grib

H. Grib

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

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M. Drouiche

M. Drouiche

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

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H. Lounici

H. Lounici

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

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N. Drouiche

Corresponding Author

N. Drouiche

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

Centre de Recherche en Technologie des Semi-conducteurs pour l'Energétique (CRTSE), 2, Bd Dr. Frantz Fanon P.O. Box 140, Algiers-7 merveilles, Algiers, 16038, Algeria

Correspondence Nadjib Drouiche, Technology of Semi-conductor for the Energetic Research Center, Department of Environmental Engineering 2, Bd Frantz Fanon BP140 Alger-7-merveilles, Algiers, Algeria. Email: [email protected]Search for more papers by this author
N. Mameri

N. Mameri

Unité de Recherche URIE, Ecole Nationale Polytechnique d'Alger, BP 182-16200, El-Harrach, Algiers, Algeria

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First published: 05 May 2017
Citations: 2

Abstract

The main purpose of this work is to study the ability of the enzymatic reaction. For the performance of the experiment, it is desirable to control variations so that they are minimized to the maximum extend and therefore an optimum operating point can be maintained. To achieve this target, the central composite design takes into account all interaction effects that may be useful for creating a mathematical model in agreement with the validity criteria. The model showed to be more suitable for the saccharification experiments in batch mode than in continuous mode. In batch mode, a correlation coefficient close to unity (R2 = .98) was obtained. Moreover, the resulting mathematical model was satisfactory in accordance with the validation criteria. For continuous mode, the coefficient value obtained was R2 = .83 indicating that the model to study the ability of the enzymatic reaction was inferior than the batch mode conditions of the analysis.

Practical applications

  • The ability of the enzymatic reaction to maintain its performance at small experimental condition variations was studied.
  • The central composite design conformed to criterion validity.
  • A correlation coefficient close to unity (R2 = .98) was obtained.

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