Effect of Poly(Lactic Acid)/Kenaf Composites Incorporated with Thymol on the Antimicrobial Activity of Processed Meat
Intan S. M. A. Tawakkal
College of Engineering and Science, Victoria University, PO Box 14428, Melbourne, 8001 Australia
Search for more papers by this authorCorresponding Author
Marlene J. Cran
Institute for Sustainability and Innovation, Victoria University, PO Box 14428, Melbourne, 8001 Australia
Corresponding author. TEL: +61 3 9919 7642; FAX: +61 3 9919 7696; EMAIL: [email protected]Search for more papers by this authorStephen W. Bigger
College of Engineering and Science, Victoria University, PO Box 14428, Melbourne, 8001 Australia
Search for more papers by this authorIntan S. M. A. Tawakkal
College of Engineering and Science, Victoria University, PO Box 14428, Melbourne, 8001 Australia
Search for more papers by this authorCorresponding Author
Marlene J. Cran
Institute for Sustainability and Innovation, Victoria University, PO Box 14428, Melbourne, 8001 Australia
Corresponding author. TEL: +61 3 9919 7642; FAX: +61 3 9919 7696; EMAIL: [email protected]Search for more papers by this authorStephen W. Bigger
College of Engineering and Science, Victoria University, PO Box 14428, Melbourne, 8001 Australia
Search for more papers by this authorAbstract
Bio-based composites comprised of poly(lactic acid) (PLA), kenaf fibers and thymol were developed and their antimicrobial (AM) properties and stability under different storage conditions investigated. The composite films containing 20-30% w/w thymol reduced E. coli in tryptone soy broth after two days at 37C and imparted a significant zone of inhibition in contact with E. coli inoculated plates. The composite films also reduced E. coli inoculated on the surface of processed sliced chicken samples after 30 days at 10C both in direct contact and in the vapour phase. The thymol additive was retained in the PLA/kenaf films that were wrapped with aluminium foil after 3 months of storage at ambient temperatures; however, unwrapped films lost some thymol to the atmosphere. The PLA/kenaf/thymol composite films show a strong potential for the development of active packaging systems in order to extend the shelf-life of some processed food products.
Practical Applications
Food packaging materials based on biopolymers are becoming increasingly popular. The synthesis of biopolymers, however, can be expensive and the resulting material properties are inferior to existing packaging materials. The addition of natural fibers can both reduce production costs and offer mechanical reinforcement to biopolymers such as poly(lactic acid) (PLA). Moreover, the addition of antimicrobial (AM) additives such as essential oil extracts can further enhance packaging functionality by preserving various foods. This study presents a packaging system comprised of a bio-derived polymer (PLA) filled with a natural and abundant fiber (kenaf) incorporating an essential oil extract (thymol) to deliver a novel material with the potential to extend the shelf-life of processed meat products.
References
- Abdul Khalil, H.P.S., Ireana Yusra, A.F., Bhat, A.H. and Jawaid, M. 2010. Cell wall ultrastructure, anatomy, lignin distribution, and chemical composition of Malaysian cultivated kenaf fiber. Ind. Crops Prod. 31, 113–121.
- Auras, R., Harte, B. and Selke, S. 2004. An overview of polylactides as packaging materials. Macromol. Biosci. 4, 835–864.
- Awal, A., Rana, M. and Sain, M. 2015. Thermorheological and mechanical properties of cellulose reinforced PLA bio-composites. Mech. Mater. 80, 87–95.
- Bachrouri, M., Quinto, E. and Mora, M. 2002. Survival of Escherichia coli O157: H7 during storage of yogurt at different temperatures. J. Food Sci. 67, 1899–1903.
- Del Nobile, M.A., Conte, A., Incoronato, A.L. and Panza, O. 2008. Antimicrobial efficacy and release kinetics of thymol from zein films. J. Food Eng. 89, 57–63.
- Emiroglu, Z.K., Yemis, G.P., Coskun, B.K. and Candogan, K. 2010. Antimicrobial activity of soy edible films incorporated with thyme and oregano essential oils on fresh ground beef patties. Meat Sci 86, 283–288.
- España, J.M., Fages, E., Moriana, R., Boronat, T. and Balart, R. 2012. Antioxidant and antibacterial effects of natural phenolic compounds on green composite materials. Polym. Compos. 33, 1288–1294.
- Fernández-Pan, I., Maté, J.I., Gardrat, C. and Coma, V. 2015. Effect of chitosan molecular weight on the antimicrobial activity and release rate of carvacrol-enriched films. Food Hydrocoll 51, 60–68.
- Guo, M., Jin, T.Z., Yadav, M.P. and Yang, R. 2015. Antimicrobial property and microstructure of micro-emulsion edible composite films against Listeria. Int. J. Food Microbiol. 208, 58–64.
- Gurunathan, T., Mohanty, S. and Nayak, S.K. 2015. A review of the recent developments in biocomposites based on natural fibres and their application perspectives. Compos. Part A 77, 1–25.
- Gutierrez, L., Escudero, A., Batlle, R. and Nerin, C. 2009. Effect of mixed antimicrobial agents and flavors in active packaging films. J. Agric. Food Chem. 57, 8564–8571.
-
Han, J.H. 2005. Antimicrobial packaging systems. In Innovations in Food Packaging ( J.H. Han, ed.) pp. 92–108, Elsevier Academic Press, San Diego, US.
10.1016/B978-012311632-1/50038-3 Google Scholar
- Jamshidian, M., Tehrany, E.A., Imran, M., Jacquot, M. and Desobry, S. 2010. Poly-lactic acid: production, applications, nanocomposites, and release studies. Compr. Rev. Food Sci. Food Saf. 9, 552–571.
- Kun, E. and Marossy, K. 2013. Evaluation methods of antimicrobial activity of plastics. Mater. Sci. Forum 729, 430–435.
- Kuorwel, K.K. 2011. Incorporation of natural antimicrobial agents into starch-based material for food packaging. PhD dissertation, Victoria University, Melbourne, Australia, 286 pp.
- Kuorwel, K.K., Cran, M.J., Sonneveld, K., Miltz, J. and Bigger, S.W. 2011. Antimicrobial activity of natural agents coated on starch-based films against Staphylococcus aureus. J. Food Sci. 76, 531–537.
- Liu, L., Fishman, M.L., Hicks, K.B. and Liu, C.K. 2005. Biodegradable composites from sugar beet pulp and poly(lactic acid). J. Agric. Food Chem. 53, 9017–9022.
-
Marcos, B.,
Sárraga, C.,
Castellari, M.,
Kappen, F.,
Schennink, G. and
Arnau, J. 2014. Development of biodegradable films with antioxidant properties based on polyesters containing α-tocopherol and olive leaf extract for food packaging applications. Food Packag. Shelf Life 1, 140–150.
10.1016/j.fpsl.2014.04.002 Google Scholar
- Muriel-Galet, V., Cran, M.J., Bigger, S.W., Hernandez-Munoz, P. and Gavara, R. 2015. Antioxidant and antimicrobial properties of ethylene vinyl alcohol copolymer films based on the release of oregano essential oil and green tea extract components. J. Food Eng. 149, 9–16.
- Muriel-Galet, V., López-Carballo, G., Gavara, R. and Hernández-Muñoz, P. 2012. Antimicrobial food packaging film based on the release of LAE from EVOH. Int. J. Food Microbiol. 157, 239–244.
- Nostro, A., Roccaro, A.S., Bisignano, G., Marino, A., Cannatelli, M.A., Pizzimenti, F.C., Cioni, P.L., Procopio, F. and Blanco, A.R. 2007. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J. Med. Microbiol. 56, 519–523.
- Otoni, C.G., Pontes, S.F.O., Medeiros, E.A.A. and Soares, N.D.F.F. 2014. Edible films from methylcellulose and nanoemulsions of clove bud (Syzygium aromaticum) and oregano (Origanum vulgare) essential oils as shelf life extenders for sliced bread. J. Agric. Food Chem. 62, 5214–5219.
- Peretto, G., Du, W.X., Avena-Bustillos, R.J., Berrios, J.D.J., Sambo, P. and Mchugh, T.H. 2014. Optimization of antimicrobial and physical properties of alginate coatings containing carvacrol and methyl cinnamate for strawberry application. J. Agric. Food Chem. 62, 984–990.
- Persico, P., Ambrogi, V., Carfagna, C., Cerruti, P., Ferrocino, I. and Mauriello, G. 2009. Nanocomposite polymer films containing carvacrol for antimicrobial active packaging. Polym. Eng. Sci. 49, 1447–1455.
- Petchwattana, N. and Naknaen, P. 2015. Utilization of thymol as an antimicrobial agent for biodegradable poly(butylene succinate). Mater. Chem. Phys. 163, 369–375.
- Pitt, J.I. and Hocking, A.D. 2009. Fungi and Food Spoilage, Springer-Verlag, New York.
- Prapruddivongs, C. and Sombatsompop, N. 2012. Roles and evidence of wood flour as an antibacterial promoter for triclosan-filled poly(lactic acid). Compos. Part B 43, 2730–2737.
- Qin, Y., Liu, D., Wu, Y., Yuan, M., Li, L. and Yang, J. 2015. Effect of PLA/PCL/cinnamaldehyde antimicrobial packaging on physicochemical and microbial quality of button mushroom (Agaricus bisporus). Postharvest Biol. Technol 99, 73–79.
- Rajesh, G., Prasad, A.R. and Gupta, A. 2015. Mechanical and degradation properties of successive alkali treated completely biodegradable sisal fiber reinforced poly lactic acid composites. J. Reinf. Plast. Compos. 34, 951–961.
- Ramesh, M. 2016. Kenaf (Hibiscus cannabinus L.) fibre based bio-materials: a review on processing and properties. Prog. Mater. Sci. 78–79, 1–92.
- Ramos, M., Beltran, A., Valdes, A., Peltzer, M.A., Jimenez, A., Garrigós, M.C. and Zaikov, G.E. 2013. Carvacrol and thymol for fresh food packaging. J. Bioequiv. Availab. 5, 154–160.
- Ramos, M., Jiménez, A., Peltzer, M. and Garrigós, M.C. 2012. Characterization and antimicrobial activity studies of polypropylene films with carvacrol and thymol for active packaging. J. Food Eng. 109, 513–519.
- Rhim, J.W. and Ng, P.K. 2007. Natural biopolymer-based nanocomposite films for packaging applications. Crit. Rev. Food Sci. Nutr. 47, 411–433.
- Risch, S.J. 2009. Food packaging history and innovations. J. Agric. Food Chem. 57, 8089–8092.
- Saba, N., Paridah, M.T. and Jawaid, M. 2015. Mechanical properties of kenaf fibre reinforced polymer composite: a review. Constr. Build. Mater. 76, 87–96.
- Sanchez-Garcia, M.D., Ocio, M.J., Gimenez, E. and Lagaron, J.M. 2008. Novel polycaprolactone nanocomposites containing thymol of interest in antimicrobial film and coating applications. J. Plast. Flim Sheet 24, 239–251.
- Shemesh, R., Goldman, D., Krepker, M., Danin-Poleg, Y., Kashi, Y., Vaxman, A. and Segal, E. 2015. LDPE/clay/carvacrol nanocomposites with prolonged antimicrobial activity. J. Appl. Polym. Sci. 132, 41261. doi: 10.1002/app.41261.
- Suppakul, P., Sonneveld, K., Bigger, S.W. and Miltz, J. 2011. Loss of AM additives from antimicrobial films during storage. J. Food Eng. 105, 270–276.
- Tao, F., Hill, L.E., Peng, Y. and Gomes, C.L. 2014. Synthesis and characterization of β-cyclodextrin inclusion complexes of thymol and thyme oil for antimicrobial delivery applications. LWT – Food Sci. Technol 59, 247–255.
- Tawakkal, I.S.M.A., Cran, M.J. and Bigger, S.W. 2014. Effect of kenaf fibre loading and thymol concentration on the mechanical and thermal properties of PLA/kenaf/thymol composites. Ind. Crops Prod 61, 74–83.
- Tawakkal, I.S.M.A., Cran, M.J. and Bigger, S.W. 2016a. Interaction and quantification of thymol in active PLA-based materials containing natural fibers. J. Appl. Polym. Sci. 133, 42160.
- Tawakkal, I.S.M.A., Cran, M.J. and Bigger, S.W. 2016b. Release of thymol from poly(lactic acid)-based antimicrobial films containing kenaf fibres as natural filler. LWT – Food Sci. Technol. 66, 629–637.
- Tawakkal, I.S.M.A., Talib, R.A., Abdan, K. and Chin, N.L. 2012. Mechanical and physical properties of kenaf derived cellulose (KDC)-filled polylactic acid (PLA) composites. Bioresources 7, 1643–1655.
- Wang, L., Liu, F., Jiang, Y., Chai, Z., Li, P., Cheng, Y., Jing, H. and Leng, X. 2011. Synergistic antimicrobial activities of natural essential oils with chitosan films. J. Agric. Food Chem. 59, 12411–12419.
- Wu, Y., Qin, Y., Yuan, M., Li, L., Chen, H., Cao, J. and Yang, J. 2014. Characterization of an antimicrobial poly(lactic acid) film prepared with poly(ε-caprolactone) and thymol for active packaging. Polym. Adv. Technol. 25, 948–954.
- Xia, X., Liu, W., Zhou, L., Liu, H., He, S. and Zhu, C. 2015. Study on flax fiber toughened poly (lactic acid) composites. J. Appl. Polym. Sci. 132, 1–10.