The effect of processing on the physicochemical properties and amino acid profile of flour from Amaranthus cruentus
Sonaal Ramsookmohan
Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa
Search for more papers by this authorSonja Venter
Agricultural Research Council-Vegetable and Ornamental Plant Institute, Pretoria, South Africa
Search for more papers by this authorCorresponding Author
John J. Mellem
Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa
Correspondence
John J. Mellem, Department of Biotechnology and Food Technology, Durban University of Technology, PO Box 1334, Durban 4000, South Africa.
Email: [email protected]
Search for more papers by this authorSonaal Ramsookmohan
Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa
Search for more papers by this authorSonja Venter
Agricultural Research Council-Vegetable and Ornamental Plant Institute, Pretoria, South Africa
Search for more papers by this authorCorresponding Author
John J. Mellem
Department of Biotechnology and Food Technology, Durban University of Technology, Durban, South Africa
Correspondence
John J. Mellem, Department of Biotechnology and Food Technology, Durban University of Technology, PO Box 1334, Durban 4000, South Africa.
Email: [email protected]
Search for more papers by this authorAbstract
Amaranth is an underutilized pseudocereal highly valued for nutritional composition. In this study, the effects of processing (soaking, boiling, and autoclaving) on the physicochemical properties on flour from Amaranthus cruentus were determined by conducting various analyses for effective ingredient applications across various food products. The amaranth flour was defatted with n-hexane in order to improve protein availability since fat forms lipid complexes with macro and micronutrients. Results showed that processing varied with regard to the impact it had on the properties of the flour. Boiling and autoclaving reduced the ash and protein content; however, the decrease in the protein content (14.23%–15.05%) was deemed insignificant. Moreover, processing treatments resulted in an increase in properties such as water absorption capacity, and in vitro digestibility (67.12%–69.71%) which is beneficial in baked products.
Practical applications
The physicochemical properties of the flour from amaranth seeds were impacted by processing with some of these changes being positive such as for the in vitro protein digestibility. The amaranth flour can be used to fortify other flours in order to obtain a desired product as well as to complement other food to increase the biological/nutrient value of processed food products.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
REFERENCES
- Adebowale, Y. A., Adeyemi, A., & Oshodi, A. A. (2005). Variability in the physicochemical, nutritional and antinutritional attributes of six Mucuna species. Food Chemistry, 89(1), 37–48. https://doi.org/10.1016/j.foodchem.2004.01.084
- Anitha, S., Govindaraj, M., & Kane-Potaka, J. (2019). Balanced amino acid and higher micronutrients in millets complements legumes for improved human dietary nutrition. Cereal Chemistry, 97(1), 74–84. https://doi.org/10.1002/cche.10227
- AOAC. (1990). Official methods of analysis ( 15th ed.). Washington, DC: Author.
- Barac, M. B., Pesic, M. B., Stanojevic, S. P., Kostic, A. Z., & Bivolarevic, V. (2015). Comparative study of the functional properties of three legume seed isolates: Adzuki, pea and soy bean. Journal of Food Science and Technology, 52(5), 2779–2787. https://doi.org/10.1007/s13197-014-1298-6
- Barba de la Rosa, A. P., Gueguen, J., Paredes-Lopez, O., & Viroben, G. (1992). Fractionation procedures, electrophoretic characterization, and amino acid composition of amaranth seed proteins. Journal of Agricultural and Food Chemistry, 40(6), 931–936. https://doi.org/10.1021/jf00018a002
- Bhat, R., & Karim, A. A. (2009). Exploring the nutritional potential of wild and underutilized legumes. Comprehensive Reviews in Food Science and Food Safety, 8(4), 305–331. https://doi.org/10.1111/j.1541-4337.2009.00084.x
- Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1006/abio.1976.9999
- Castel, V., Andrich, O., Netto, F. M., Santiago, L. G., & Carrara, C. R. (2012). Comparison between isoelectric precipitation and ultrafiltration processes to obtain Amaranth mantegazzianus protein concentrates at pilot plant scale. Journal of Food Engineering, 112(4), 288–295. https://doi.org/10.1016/j.jfoodeng.2012.05.010
- Damodaran, S. (2017). Protein-stabilized foams and emulsions. In Food proteins and their applications (pp. 57–110). Boca Raton, FL: CRC Press.
10.1201/9780203755617-3 Google Scholar
- Dickinson, E. (1994). Protein-stabilized emulsions. In Water in foods ( 59–74). Pergamon.
10.1016/B978-1-85861-037-5.50010-2 Google Scholar
- Elsohaimy, S. A., Refaay, T. M., & Zaytoun, M. A. M. (2015). Physicochemical and functional properties of quinoa protein isolate. Annals of Agricultural Sciences, 60(2), 297–305. https://doi.org/10.1016/j.aoas.2015.10.007
10.1016/j.aoas.2015.10.007 Google Scholar
- Esan, Y. O., Omoba, O. S., & Enujiugha, V. N. (2018). Biochemical and nutritional compositions of two accessions of Amaranthus cruentus seed flour. American Journal of Food Science and Technology, 6(4), 145–150. https://doi.org/10.12691/ajfst-6-4-3
- Folasade, A. A., & Olorunfemi, O. F. (2019). Proximate analysis, mineral contents and amino acid composition of Antrocaryon micraster stem bark. Journal of Agriculture and Environmental Sciences, 8(2), 49–59. https://doi.org/10.15640/jaes.v8n2a7
10.15640/jaes.v8n2a7 Google Scholar
- García-Salcedo, Á. J., Torres-Vargas, O. L., & Ariza-Calderón, H. (2018). Physical-chemical characterization of quinoa (Chenopodium quinoa Willd.), amaranth (Amaranthus caudatus L.), and chia (Salvia hispanica L.) flours and seeds. Acta Agronómica, 67(2), 215–222 https://doi.org/10.15446/acag.v67n2.63666
10.15446/acag.v67n2.63666 Google Scholar
- Graham, D. E., & Phillips, M. C. (1976). The conformation of proteins at interfaces and their role in stabilizing emulsions. In Theory and practice of emulsion technology (pp. 75–98). London, UK: Academic Press.
10.1016/B978-0-12-651250-2.50010-7 Google Scholar
- Gupta, S., Chhabra, G. S., Liu, C., Bakshi, J. S., & Sathe, S. K. (2018). Functional properties of select dry bean seeds and flours. Journal of Food Science, 83(8), 2052–2061. https://doi.org/10.1111/1750-3841.14213
- Hamid, S., Muzzafar, S., Wani, I. A., & Masoodi, F. A. (2015). Physicochemical and functional properties of two cowpea cultivars grown in temperate Indian climate. Cogent Food & Agriculture, 1(1), 1099418. https://doi.org/10.1080/23311932.2015.1099418
10.1080/23311932.2015.1099418 Google Scholar
- Hassan, A. B., von Hoersten, D., & Ahmed, I. A. M. (2019). Effect of radio frequency heat treatment on protein profile and functional properties of maize grain. Food Chemistry, 271, 142–147. https://doi.org/10.1016/j.foodchem.2018.07.190
- Iyenagbe, D. O., Malomo, S. A., Idowu, A. O., Badejo, A. A., & Fagbemi, T. N. (2017). Effects of thermal processing on the nutritional and functional properties of defatted conophor nut (Tetracarpidium conophorum) flour and protein isolates. Food Science & Nutrition, 5(6), 1170–1178. https://doi.org/10.1002/fsn3.508
- Jimoh, M. O., Afolayan, A. J., & Lewu, F. B. (2018). Suitability of Amaranthus species for alleviating human dietary deficiencies. South African Journal of Botany, 115, 65–73. https://doi.org/10.1016/j.sajb.2018.01.004
- Khan, S. H., Butt, M. S., Sharif, M. K., Sameen, A., Mumtaz, S., & Sultan, M. T. (2011). Functional properties of protein isolates extracted from stabilized rice bran by microwave, dry heat, and parboiling. Journal of Agricultural and Food Chemistry, 59(6), 2416–2420. https://doi.org/10.1021/jf104177x
- Kinsella, J. E., & Melachouris, N. (1976). Functional properties of proteins in foods: A survey. Critical Reviews in Food Science & Nutrition, 7(3), 219–280. https://doi.org/10.1080/10408397609527208
- Li, C., Yang, J., Yao, L., Qin, F., Hou, G., Chen, B., … Shen, Y. (2020). Characterisation, physicochemical and functional properties of protein isolates from Amygdalus pedunculata Pall seeds. Food Chemistry, 311, 125888. https://doi.org/10.1016/j.foodchem.2019.125888
- Ma, Z., Boye, J. I., Simpson, B. K., Prasher, S. O., Monpetit, D., & Malcolmson, L. (2011). Thermal processing effects on the functional properties and microstructure of lentil, chickpea, and pea flours. Food Research International, 44(8), 2534–2544. https://doi.org/10.1016/j.foodres.2010.12.017
- Mizutani, Y., Shibata, M., Yamada, S., Nambu, Y., Hirotsuka, M., & Matsumura, Y. (2019). Effects of heat treatment under low moisture conditions on the protein and oil in soybean seeds. Food Chemistry, 275, 577–584. https://doi.org/10.1016/j.foodchem.2018.09.139
- Mota, C., Santos, M., Mauro, R., Samman, N., Matos, A. S., Torres, D., & Castanheira, I. (2016). Protein content and amino acids profile of pseudocereals. Food Chemistry, 193, 55–61. https://doi.org/10.1016/j.foodchem.2014.11.043
- Myers, N. (2002). Environmental refugees: A growing phenomenon of the 21st century. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 357(1420), 609–613. https://doi.org/10.1098/rstb.2001.0953
- Naidoo, T., Gerrano, A. S., & Mellem, J. J. (2017). The effect of processing on in vitro protein and starch digestibility and predictive glycaemic index of five Vigna unguiculata (cowpea) cultivars. The Annals of the University of Dunarea De Jos of Galati. Fascicle VI. Food Technology, 41(2), 31–41.
- Naiker, T. S., Gerrano, A., & Mellem, J. (2019). Physicochemical properties of flour produced from different cowpea (Vigna unguiculata) cultivars of Southern African origin. Journal of Food Science and Technology, 56(3), 1541–1550. https://doi.org/10.1007/s13197-019-03649-1
- Obi, C. D., & Okoye, J. I. (2017). Effects of boiling and autoclaving on the chemical composition and functional properties of Mucuna Flagellipes seed flours. International Journal of Innovative Food, Nutrition & Sustainable Agriculture, 5(2), 18–24.
- Oser, B. L. (1959). An integrated essential amino acid index for predicting the biological value of proteins. In Protein and amino acid nutrition (pp. 295–311). New York, NY: Academic Press.
10.1016/B978-0-12-395683-5.50014-6 Google Scholar
- Oyeyinka, S. A., Singh, S., & Amonsou, E. O. (2017). Physicochemical properties of starches extracted from bambara groundnut landraces. Starch-Stärke, 69(3–4), 1600089. https://doi.org/10.1002/star.201600089
- Prinyawiwatkul, W., Beuchat, L. R., McWatters, K. H., & Phillips, R. D. (1997). Functional properties of cowpea (Vigna unguiculata) flour as affected by soaking, boiling, and fungal fermentation. Journal of Agricultural and Food Chemistry, 45(2), 480–486. https://doi.org/10.1021/jf9603691
- Sanchiz, A., Pedrosa, M. M., Guillamón, E., Arribas, C., Cabellos, B., Linacero, R., & Cuadrado, C. (2019). Influence of boiling and autoclave processing on the phenolic content, antioxidant activity and functional properties of pistachio, cashew and chestnut flours. Lwt–food Science and Technology, 105, 250–256. https://doi.org/10.1016/j.lwt.2019.02.035
- Sathe, S. K., Deshpande, S. S., & Salunkhe, D. K. (1982). Functional properties of winged bean [Psophocarpus tetragonolobus (L.) DC] proteins. Journal of Food Science, 47(2), 503–509. https://doi.org/10.1111/j.1365-2621.1982.tb10112.x
- Sharma, P., Singh, V., & Subramanian, R. (2013). Pasting, swelling, and solubility characteristics of rice batter prepared from different wet grinding systems. Starch-Stärke, 65(5–6), 374–381. https://doi.org/10.1002/star.201200126
- Shevkani, K., Kaur, A., Kumar, S., & Singh, N. (2015). Cowpea protein isolates: Functional properties and application in gluten-free rice muffins. Lwt—food Science and Technology, 63(2), 927–933. https://doi.org/10.1016/j.lwt.2015.04.058
- Shevkani, K., Singh, N., Kaur, A., & Rana, J. C. (2014). Physicochemical, pasting, and functional properties of amaranth seed flours: Effects of lipids removal. Journal of Food Science, 79(7), C1271–C1277. https://doi.org/10.1111/1750-3841.12493
- Sindhu, R., Beniwal, S. K. Band, & Devi, A. (2019). Effect of grain processing on nutritional and physico-chemical, functional and pasting properties of amaranth and quinoa flours. Indian Journal of Traditional Knowledge (IJTK), 18(3), 500–507.
- Singh, N., Singh, P., Shevkani, K., & Virdi, A. S. (2019). Amaranth: Potential source for flour enrichment. In Flour and breads and their fortification in health and disease prevention (pp. 123–135). London, UK: Academic Press.
10.1016/B978-0-12-814639-2.00010-1 Google Scholar
- Tinus, T., Damour, M., Van Riel, V., & Sopade, P. A. (2012). Particle size-starch-protein digestibility relationships in cowpea (Vigna unguiculata). Journal of Food Engineering, 113(2), 254–264. https://doi.org/10.1016/j.jfoodeng.2012.05.041