A new method, checkMySequence, for the fast and automated detection of register errors in protein models built into cryo-EM reconstructions is presented.

In this article, it is shown that high-throughput strategies for tomographic data acquisition combined with unsupervised techniques for image analysis provide the foundation for closing the resolution gap between the high-resolution strategies used to study molecular assemblies reconstituted in vitro and techniques for in situ structure determination.

Crystal structures of TEM-171 and its complexes with tazobactam reveal a novel structural intermediate in the enzyme-inhibition process.

Crystal structures of apo and trehalose-bound forms of LpqY from Mycobacterium tuberculosis are presented at 2.2 and 1.9 Å resolution, respectively. The structural analysis, together with in silico and binding-affinity analyses, show the specificity and stereoselectivity of Mtb-LpqY for trehalose over maltose.

The crystal structure of dihydrodipicolinate synthase modified with (S)-2-bromopropionate at Lys161 is reported.

Structures of the yeast Sec14-like phosphatidylinositol transfer protein Sfh2 in complex with phosphatidylinositol and squalene reveal structural determinants for ligand recognition.

Structural and biochemical analyses of S-adenosyl-l-homocysteine hydolase from Synechocystis sp. PCC 6803 are reported.

Microsecond melting and revitrification of cryo samples preserves the structure of embedded particles. The beam-induced motion of revitrified samples is comparable to that of conventional cryo samples.

A new software package, autoPX, for processing X-ray diffraction data from biomacromolecular crystals is introduced. It adopts several novel methods and provides adequate solutions to problems encountered at the SSRF beamlines.

This paper describes the design and assessment of CryoVR, a virtual reality-based training system for cryoEM devices. CryoVR provides a unique learning opportunity by giving users experience of cryoEM experimental procedures through a safe and accessible virtual environment.

Random mutagenesis at designated sites of the l-asparaginase EcAIII was combined with crystal structure analysis and AlphaFold2 predictions to dissect the enzyme-maturation and catalytic processes.