The focused issue on Empowering education through structural genomics is introduced. The virtual issue is available at https://journals.iucr.org/special_issues/2024/educationsg.

The purification, crystallization and three crystal structures of the putative endoribonuclease L-PSP from E. histolytica are reported.

Fe²⁺ binding stabilizes collagen lysyl hydroxylase dimers, although the underlying mechanism remains unclear. Here, we report the crystal structure of an Fe²⁺-binding-deficient mimiviral collagen lysyl hydroxylase, highlighting the conformational changes upon loss of Fe²⁺ binding.

The crystal structure of a variant of recombinant A. bisporus mannose-binding protein with a longer C-terminal region was determined. The flexible C-terminal region may influence the sugar-binding ability.

We have determined the structure of the Rhizobium phage vB_RleM_P10VF DNA ligase bound to a nicked DNA duplex to 2.2 Å resolution. The DNA ligase exhibits a canonical DNA ligase-binding mode fully encircling the duplex and has considerable structural homology to T4 DNA ligase and the bacterial ATP-dependent DNA ligase from Prochlorococcus marinus.

The 1.7 Å resolution structure of the C1 domain of the surface-layer protein SlpM from L. brevis is reported. The contact surfaces observed in the crystal lattice potentially contribute to the self-assembly process of SlpM.

YcdY, a putative chaperone of the NarJ subfamily, forms a helix-bundle structure characterized by a dent on the concave side. The dent contains hydrophobic or conserved residues, presumably for the chaperone function of YcdY. Furthermore, we propose that YcdY may function as a chaperone for proteins other than the previously proposed YcdX.