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introduction

Open Access

Two way street – complementary methods

Ivo Tews,  Jon Cooper, 
  • Pages: 1-2
  • First Published: 23 January 2015

biophysics

Open Access

Combining biophysical methods for the analysis of protein complex stoichiometry and affinity in SEDPHAT

Huaying Zhao,  Peter Schuck, 
  • Pages: 3-14
  • First Published: 23 January 2015
Open Access

In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF

David von Stetten,  Thierry Giraud,  Philippe Carpentier,  Franc Sever,  Maxime Terrien,  Fabien Dobias,  Douglas H. Juers,  David Flot,  Christoph Mueller-Dieckmann,  Gordon A. Leonard,  Daniele de Sanctis,  Antoine Royant, 
  • Pages: 15-26
  • First Published: 23 January 2015
Open Access

Challenges and solutions for the analysis of in situ, in crystallo micro-spectrophotometric data

Florian S. N. Dworkowski,  Michael A. Hough,  Guillaume Pompidor,  Martin R. Fuchs, 
  • Pages: 27-35
  • First Published: 23 January 2015
Open Access

Protein–ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry (DPI)

Morten K. Grøftehauge,  Nelly R. Hajizadeh,  Marcus J. Swann,  Ehmke Pohl, 
  • Pages: 36-44
  • First Published: 23 January 2015

SAXS

Open Access

The accurate assessment of small-angle X-ray scattering data

Thomas D. Grant,  Joseph R. Luft,  Lester G. Carter,  Tsutomu Matsui,  Thomas M. Weiss,  Anne Martel,  Edward H. Snell, 
  • Pages: 45-56
  • First Published: 23 January 2015
Open Access

Uniqueness of models from small-angle scattering data: the impact of a hydration shell and complementary NMR restraints

Henry S. Kim,  Frank Gabel, 
  • Pages: 57-66
  • First Published: 23 January 2015
Open Access

BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments

Adam Round,  Franck Felisaz,  Lukas Fodinger,  Alexandre Gobbo,  Julien Huet,  Cyril Villard,  Clement E. Blanchet,  Petra Pernot,  Sean McSweeney,  Manfred Roessle,  Dmitri I. Svergun,  Florent Cipriani, 
  • Pages: 67-75
  • First Published: 23 January 2015
Open Access

ISPyB for BioSAXS, the gateway to user autonomy in solution scattering experiments

Alejandro De Maria Antolinos,  Petra Pernot,  Martha E. Brennich,  Jérôme Kieffer,  Matthew W. Bowler,  Solange Delageniere,  Staffan Ohlsson,  Stephanie Malbet Monaco,  Alun Ashton,  Daniel Franke,  Dmitri Svergun,  Sean McSweeney,  Elspeth Gordon,  Adam Round, 
  • Pages: 76-85
  • First Published: 23 January 2015
Open Access

Memprot: a program to model the detergent corona around a membrane protein based on SEC–SAXS data

Javier Pérez,  Alexandros Koutsioubas, 
  • Pages: 86-93
  • First Published: 23 January 2015

new mounts

Open Access

Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening

Christina M. Cuttitta,  Daniel L. Ericson,  Alexander Scalia,  Christian G. Roessler,  Ella Teplitsky,  Karan Joshi,  Olven Campos,  Rakhi Agarwal,  Marc Allaire,  Allen M. Orville,  Robert M. Sweet,  Alexei S. Soares, 
  • Pages: 94-103
  • First Published: 23 January 2015
Open Access

Experimental phasing for structure determination using membrane-protein crystals grown by the lipid cubic phase method

Dianfan Li,  Valerie E. Pye,  Martin Caffrey, 
  • Pages: 104-122
  • First Published: 23 January 2015

EM

Open Access

Collaborative Computational Project for Electron cryo-Microscopy

Chris Wood,  Tom Burnley,  Ardan Patwardhan,  Sjors Scheres,  Maya Topf,  Alan Roseman,  Martyn Winn, 
  • Pages: 123-126
  • First Published: 23 January 2015
Open Access

A national facility for biological cryo-electron microscopy

Helen R. Saibil,  Kay Grünewald,  David I. Stuart, 
  • Pages: 127-135
  • First Published: 23 January 2015
Open Access

Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

Alan Brown,  Fei Long,  Robert A. Nicholls,  Jaan Toots,  Paul Emsley,  Garib Murshudov, 
  • Pages: 136-153
  • First Published: 23 January 2015

dynamics

Open Access

Structure calculation, refinement and validation using CcpNmr Analysis

Simon P. Skinner,  Benjamin T. Goult,  Rasmus H. Fogh,  Wayne Boucher,  Tim J. Stevens,  Ernest D. Laue,  Geerten W. Vuister, 
  • Pages: 154-161
  • First Published: 23 January 2015
Open Access

In pursuit of an accurate spatial and temporal model of biomolecules at the atomistic level: a perspective on computer simulation

Alan Gray,  Oliver G. Harlen,  Sarah A. Harris,  Syma Khalid,  Yuk Ming Leung,  Richard Lonsdale,  Adrian J. Mulholland,  Arwen R. Pearson,  Daniel J. Read,  Robin A. Richardson, 
  • Pages: 162-172
  • First Published: 23 January 2015