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RESEARCH ARTICLES
Improved N-acetylneuraminic acid bioproduction by optimizing pathway for reducing intermediate accumulation
- Pages: 205-211
- First Published: 14 November 2022

1. To eliminate the extensively accumulated by-product N-acetylglucosamine (GlcNAc), the UDP-GlcNAc epimerase (NeuC) pathway without GlcNAc production was firstly reconstructed and optimized in Bacillus subtilis, and thereby NeuAc titer increased to 5.9 g/L and the by-product GlcNAc was eliminated. 2. The maximum titer of NeuAc in fed-batch fermentation in a 3-L fermenter was 21.8 g/L.
Hydroxyl radical enhanced carbon dots fluorescence quenching immunoassays for simultaneous detection of six kinds of antibiotics
- Pages: 212-223
- First Published: 14 November 2022

A label-free fluorescence quenching immunoassays using hydroxyl radical-sensitive carbon dots as fluorescence probe was established for simultaneous extraction and highly sensitive detection of six wide-spectrum antibiotics (nitrofuran metabolites, chloramphenicol, and florfenicol) in animal-derived food. The organic combination of •OH-sensitive CDs, high-sensitivity fluorescence quenching immunoassays, and efficient pretreatment methods provides support for the development and application of new immunoassay technology.
Immunomodulatory activity of mannosylglycerate and two unnatural mannosyl-oligosaccharides obtained from microbial fermentation on RAW264.7 macrophages
- Pages: 224-232
- First Published: 29 August 2022

(a) Mannosyl compounds obtained from microbial fermentation exhibited immunomodulatory activities in the innate and adaptive immune systems. (b) The immunomodulatory activity of mannosyl compounds on RAW 264.7 cells mainly functioned through mitogen-activated protein kinases and MyD88-dependent signaling pathways. (c) The length of the mannosyl chain and the type of glycosidic bounds influence the function of mannan oligosaccharides.
Analysis of cofermentation characteristics of Lactobacillus bulgaricus and Streptococcus thermophilus based on microrheology
- Pages: 233-240
- First Published: 02 December 2022

The microrheological properties of different starter combinations (Lactobacillus bulgaricus and Streptococcus thermophilus) in the milk fermentation process were analyzed by multifrequency diffusion spectroscopy. Meanwhile, the fermented milk's stability, texture, and sensory characteristics were also observed during storage. This study will provide data support and methodology for developing and screening compound starters applied for milk fermentation in the future.
REVIEW ARTICLE
Biological functions of compounds from Bacillus subtilis and its subspecies, Bacillus subtilis natto
- Pages: 241-251
- First Published: 07 December 2022

Natto, produced by fermentation of soybeans by B. subtilis natto (a subspecies of B. subtilis), has been consumed as a traditional fermented food for at least a thousand years. Natto is attracting interest as a potential functional food for health. This review summarizes compounds and its functions of B. subtilis natto.
RESEARCH ARTICLES
Lactiplantibacillus plantarum X7022 ameliorates loperamide-induced constipation and modulates gut microbiota in mice
- Pages: 252-263
- First Published: 02 November 2022
Moringa leaves for improving the health benefits of quinoa fermented by probiotics
- Pages: 264-275
- First Published: 08 December 2022

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Supplementation with Moringa leaves powder (MLP) enhances the growth and survival of probiotics on the fermented quinoa.
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Adding of MLP to fermented quinoa increases its phenolic content and antioxidant activity.
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Feeding with fermented quinoa containing of MLP improves the lipid profile of experimental rats.
Characterization of biodegradable food packaging films prepared with polyamide 4: Influence of molecular weight and environmental humidity
- Pages: 276-288
- First Published: 02 September 2022

Colorless and translucent polyamide 4 (PA4) films, which are both bio-based and biodegradable, were prepared by the solution casting method in a dual-solvent system. The physical properties of single PA4 films were originally characterized, and the effects of molecular weight and environmental humidity on PA4 were investigated.
Effects of thermal and nonthermal processing techniques on aroma compounds in fruit juices: A meta-analysis
- Pages: 289-297
- First Published: 08 December 2022
The purification and identification of antioxidants and dipeptidyl peptidase IV inhibitory peptides from whey protein hydrolysates
- Pages: 298-306
- First Published: 11 October 2022

Whey protein was enzymatically hydrolyzed using an ultrahigh-pressure synergistic enzymolysis method. The identified amino acid sequences were then synthesized, and their antioxidants and DPP-IV inhibitory activities were determined. Whey protease hydrolysates could potentially be expected to be successfully commercially developed as functional peptides in the future.
Nitrite-reducing performance of two Lactobacillus strains isolated from traditional Sichuan fermented sausage in different production processes
- Pages: 307-318
- First Published: 14 September 2022

This study screened Pediococcus pentosaceus and Leuconostoc mesenteroides insects in traditional Sichuan sausages and determined their nitrite-reducing properties during different production processes. These two strains are expected to be the starting point for the improvement of biological safety and nitrite residue control in traditional Sichuan sausage production.
Improving textural properties of gluten-free veggie sausage with egg white proteins
- Pages: 319-330
- First Published: 11 October 2022