Analysis on folding of misgurin using two-dimensional HP model
Shaomin Yan
State Key Laboratory of Nonfood Biomass Enzyme Technology, National Engineering Research Center for Nonfood Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China
Search for more papers by this authorCorresponding Author
Guang Wu
State Key Laboratory of Nonfood Biomass Enzyme Technology, National Engineering Research Center for Nonfood Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China
DreamSciTech Consulting, 301, Building 12, Nanyou A-zone, Jiannan Road, Shenzhen, Guangdong, 518054, China
State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Nonfood Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China===Search for more papers by this authorShaomin Yan
State Key Laboratory of Nonfood Biomass Enzyme Technology, National Engineering Research Center for Nonfood Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China
Search for more papers by this authorCorresponding Author
Guang Wu
State Key Laboratory of Nonfood Biomass Enzyme Technology, National Engineering Research Center for Nonfood Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi 530007, China
DreamSciTech Consulting, 301, Building 12, Nanyou A-zone, Jiannan Road, Shenzhen, Guangdong, 518054, China
State Key Laboratory of Non-food Biomass Enzyme Technology, National Engineering Research Center for Nonfood Biorefinery, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, Nanning, Guangxi 530007, China===Search for more papers by this authorAbstract
Misgurin is an antimicrobial peptide from the loach, while the hydrophobic-polar (HP) model is a way to study the folding conformations and native states in peptide and protein although several amino acids cannot be classified either hydrophobic or polar. Practically, the HP model requires extremely intensive computations, thus it has yet to be used widely. In this study, we use the two-dimensional HP model to analyze all possible folding conformations and native states of misgurin with conversion of natural amino acids according to the normalized amino acid hydrophobicity index as well as the shortest benchmark HP sequence. The results show that the conversion of misgurin into HP sequence with glycine as hydrophobic amino acid at pH 2 has 1212 folding conformations with the same native state of minimal energy –6; the conversion of glycine as polar amino acid at pH 2 has 13,386 folding conformations with three native states of minimal energy –5; the conversion of glycine as hydrophobic amino acid at pH 7 has 2538 folding conformations with three native states of minimal energy –5; and the conversion of glycine as polar amino acid at pH 7 has 12,852 folding conformations with three native states of minimal energy –4. Those native states can be ranked according to the normalized amino acid hydrophobicity index. The detailed discussions suggest two ways to modify misgurin. Proteins 2011. © 2012 Wiley Periodicals, Inc.
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