Systematic evaluation of the biological variance within the Raman based colorectal tissue diagnostics
Nadine Vogler
Leibniz Institute of Photonic Technology, 07745 Jena, Germany
Search for more papers by this authorCorresponding Author
Thomas Bocklitz
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, 07743 Jena, Germany
Corresponding author: e-mail: [email protected]
Search for more papers by this authorFiras Subhi Salah
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Iraqi Centre for Cancer and Medical Genetics Research, Al-Mustansiriya University, Baghdad, Iraq
Search for more papers by this authorCarsten Schmidt
Clinic for Internal Medicine IV, Jena University Hospital, 07747 Jena, Germany
Search for more papers by this authorRolf Bräuer
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Search for more papers by this authorTiantian Cui
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA
Search for more papers by this authorMasoud Mireskandari
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Search for more papers by this authorFlorian R. Greten
Institute for Tumor Biology and Experimental Therapy, Georg-Speyer-Haus, 60596 Frankfurt, Germany
Search for more papers by this authorMichael Schmitt
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, 07743 Jena, Germany
Search for more papers by this authorAndreas Stallmach
Clinic for Internal Medicine IV, Jena University Hospital, 07747 Jena, Germany
Search for more papers by this authorIver Petersen
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Search for more papers by this authorJürgen Popp
Leibniz Institute of Photonic Technology, 07745 Jena, Germany
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, 07743 Jena, Germany
Search for more papers by this authorNadine Vogler
Leibniz Institute of Photonic Technology, 07745 Jena, Germany
Search for more papers by this authorCorresponding Author
Thomas Bocklitz
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, 07743 Jena, Germany
Corresponding author: e-mail: [email protected]
Search for more papers by this authorFiras Subhi Salah
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Iraqi Centre for Cancer and Medical Genetics Research, Al-Mustansiriya University, Baghdad, Iraq
Search for more papers by this authorCarsten Schmidt
Clinic for Internal Medicine IV, Jena University Hospital, 07747 Jena, Germany
Search for more papers by this authorRolf Bräuer
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Search for more papers by this authorTiantian Cui
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Department of Radiology, The Ohio State University Wexner Medical Center, Columbus, OH, USA
Search for more papers by this authorMasoud Mireskandari
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Search for more papers by this authorFlorian R. Greten
Institute for Tumor Biology and Experimental Therapy, Georg-Speyer-Haus, 60596 Frankfurt, Germany
Search for more papers by this authorMichael Schmitt
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, 07743 Jena, Germany
Search for more papers by this authorAndreas Stallmach
Clinic for Internal Medicine IV, Jena University Hospital, 07747 Jena, Germany
Search for more papers by this authorIver Petersen
Institute of Pathology, Jena University Hospital, 07743 Jena, Germany
Search for more papers by this authorJürgen Popp
Leibniz Institute of Photonic Technology, 07745 Jena, Germany
Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, 07743 Jena, Germany
Search for more papers by this authorAbstract
Being among the most common cancers worldwide screening and early diagnosis of colorectal cancer is of high interest for the health system, the patients and for research. Raman microspectroscopy as a label-free, non-invasive and non-destructive technique is a promising tool for an early diagnosis. However, to ensure a reliable diagnosis specially designed statistical analysis workflows are required. Several statistical approaches have been introduced leading to varying results in the overall accuracy, sensitivity and specificity. In this study a systematic evaluation of different statistical analysis approaches has been performed using a colon cancer mouse model with genotypic identical individuals. Based on the inter-individual Raman spectral variances a measure for the biological variance can be estimated. By applying a leave-one-individual-out cross-validation a clinically relevant discrimination of healthy tissue versus adenoma and carcinoma with an accuracy of 95% is shown. Furthermore, the transfer of a model from tissue to biopsy specimen is demonstrated.
Supporting Information
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| jbio201500237-sup-0001-author-biographies.pdfPDF document, 575.7 KB | Author Biographies |
| jbio201500237-sup-0002-supp-information.pdfPDF document, 418.3 KB | Supporting Information |
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