Sex and age discrepancy of HbA1c and fetal hemoglobin determined by HPLC in a large Chinese Han population
利用HPLC法测定大样本量中国汉族人群糖化血红蛋白与胎儿血红蛋白的性别和年龄差异
Abstract
enBackground
It is accepted that HbA1c is an effective indicator to evaluate glycemic control. Fetal hemoglobin (HbF) is seldom measured because traditional detection is inconvenient. In this regard, there may be an advantage in using HPLC autoanalysis of HbA1c as a surrogate method for HbF detection. The aim of the present study was to explore the distribution of HbA1c and HbF in a large Chinese Han population.
Methods
In all, 70 553 blood samples were collected between January 2012 and June 2016. Study subjects were inpatients undergoing routine medical care and were divided into four groups based on age: Group A, 20–39 years; Group B, 40–59 years; Group C, 60–79 years; and Group D, ≥80 years. Blood HbA1c and HbF concentrations were measured by HPLC using a Tosho Bioscience (Tokyo, Japan) G8 analyzer.
Results
There was a positive association between HbA1c and age, and a negative association between HbF and age. The concentration range of HbF was narrow and HbF concentrations were significantly higher in females than males, regardless of age (median 0.7% vs 0.6%, respectively; P < 0.0001). There was a low degree of correlation between HbF and HbA1c (r = 0.181, P < 0.0001). Although median HbA1c levels were higher in male than female subjects aged 20–59 years (5.5% vs 5.4%, respectively, in Group A; 5.9% vs 5.8%, respectively in Group B), in the 60–79 years group, HbA1c levels were lower in males than females (6.1% vs 6.2%, respectively; P < 0.0001).
Conclusions
The data suggest that sex and age should be considered in clinical interpretation of HbA1c.
摘要
zh背景
糖化血红蛋白(HbA1c)被公认为是评估血糖控制的有效指标。胎儿血红蛋白(fetal hemoglobin, HbF)的传统检测方法较为繁琐, 故临床上很少作为常规检查项目。利用高效液相色谱法(High Performance Liquid Chromatography, HPLC)自动分析糖化血红蛋白可作为检测HbF的一种替代方法。本研究旨在探索大样本的中国汉族人群中, HbA1c和HbF的分布情况。
方法
我们自2012年1月至2016年6月期间共收集了70553份血液样本。研究对象为接受常规治疗的住院患者, 根据年龄分为四组:A组, 20-39岁;B组, 40-59岁;C组, 60-79岁和D组, ≥ 80岁。血液HbA1c和HbF浓度利用HPLC法测量, 使用Tosho Bioscience(Tokyo, Japan)G8分析仪。
结果
HbA1c与年龄呈正相关, HbF与年龄呈负相关。HbF浓度分布较集中, 且所有年龄段的女性HbF浓度显著高于男性(分别为0.7%和0.6%, P < 0.0001)。HbF和HbA1c之间的相关性很低(r = 0.181, P < 0.0001)。尽管20-59岁的男性HbA1c中位数高于女性(A组分别为5.5%和5.4%, B组分别为5.9%和5.8%), 但在60-79岁组中, 男性HbA1c的水平低于女性(分别为6.1%和6.2%, P < 0.0001)。
结论
本研究的结果表明, 在临床实践中解读HbA1c或HbF时应考虑性别和年龄的影响。
