Optical coherence hyperspectral microscopy with a single supercontinuum light source
Funding information: National Natural Science Foundation of China, Grant/Award Numbers: 61627827, 81630046, 91539127, 61705068; Science and Technology Program of Guangzhou, Grant/Award Number: 2019050001; The National High Technology Research and Development Program of China, Grant/Award Number: 2015AA020901; The Ph.D. Start-up Fund of Natural Science Foundation of Guangdong Province, Grant/Award Number: 2017A030310363; The Science and Technology Planning Project of Guangdong Province, China, Grant/Award Numbers: 2015B020233016, 2014B020215003
Abstract
In the paper, we have developed an optical coherence hyperspectral microscopy with a single supercontinuum light source. The microscopy consists of optical coherence tomography (OCT) and hyperspectral imaging (HSI), which can visualize the structural and functional characteristics of biological tissues. The 500 to 700 nm band is selected for HSI and OCT imaging, where HSI enables imaging of oxygen saturation and hemoglobin (Hb) content, while OCT acquires structural characteristics to assess the morphology of biological tissues. The system performance of the optical coherence hyperspectral microscopy is verified by normal mice ears, and the practical applications of the microscopy is further performed in 4T1 and inflammation Balb/c mice ears in vivo. The experimental results demonstrate that the microscopy has potential to provide complementary information for clinical applications.
