Label-free discrimination and selection of cancer cells from blood during flow using holography-induced dielectrophoresis
Matan Dudaie
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Search for more papers by this authorNoga Nissim
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Search for more papers by this authorItay Barnea
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Search for more papers by this authorTobias Gerling
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany
Search for more papers by this authorClaus Duschl
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany
Search for more papers by this authorMichael Kirschbaum
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany
Search for more papers by this authorCorresponding Author
Natan T. Shaked
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Correspondence
Natan T. Shaked, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Ramat Aviv, 69978 Tel Aviv, Israel.
Email: [email protected]
Search for more papers by this authorMatan Dudaie
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Search for more papers by this authorNoga Nissim
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Search for more papers by this authorItay Barnea
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Search for more papers by this authorTobias Gerling
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany
Search for more papers by this authorClaus Duschl
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany
Search for more papers by this authorMichael Kirschbaum
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany
Search for more papers by this authorCorresponding Author
Natan T. Shaked
Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel
Correspondence
Natan T. Shaked, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Ramat Aviv, 69978 Tel Aviv, Israel.
Email: [email protected]
Search for more papers by this authorFunding information: Israel and German Ministries of Economy and Industry
Abstract
We present a method for label-free imaging and sorting of cancer cells in blood, which is based on a dielectrophoretic microfluidic chip and label-free interferometric phase microscopy. The chip used for imaging has been embedded with dielectrophoretic electrodes, and therefore it can be used to sort the cells based on the decisions obtained during the cell flow by the label-free quantitative imaging method. Hence, we obtained a real-time, automatic, label-free imaging flow cytometry with the ability to sort the cells during flow. To validate our model, we combined into the label-free imaging interferometer a fluorescence imaging channel that indicated the correctness of the label-free sorting. We have achieved above 98% classification success and 69% sorting accuracy at flow rates of 4 to 7 μL hr−1. In the future, this method is expected to help in label-free sorting of circulating tumor cells in blood following an initial state-of-the-art cell enrichment.
CONFLICT OF INTEREST
The authors declare no potential conflict of interest.
Supporting Information
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