Characterization of hydroxypropyl methyl cellulose–whey protein concentrate bionanocomposite films reinforced by chitosan nanoparticles
Corresponding Author
Mahsa Shojaei
Department of Food Science & Technology, College of Agriculture, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran
Correspondence
Mahsa Shojaei and Mohammadreza Eshaghi, Department of Food Science & Technology, College of Agriculture, Varamin - Pishva branch, Islamic Azad University, Varamin, Iran.
Email: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Mohammadreza Eshaghi
Department of Food Science & Technology, College of Agriculture, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran
Correspondence
Mahsa Shojaei and Mohammadreza Eshaghi, Department of Food Science & Technology, College of Agriculture, Varamin - Pishva branch, Islamic Azad University, Varamin, Iran.
Email: [email protected], [email protected]
Search for more papers by this authorLeila Nateghi
Department of Food Science & Technology, College of Agriculture, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran
Search for more papers by this authorCorresponding Author
Mahsa Shojaei
Department of Food Science & Technology, College of Agriculture, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran
Correspondence
Mahsa Shojaei and Mohammadreza Eshaghi, Department of Food Science & Technology, College of Agriculture, Varamin - Pishva branch, Islamic Azad University, Varamin, Iran.
Email: [email protected], [email protected]
Search for more papers by this authorCorresponding Author
Mohammadreza Eshaghi
Department of Food Science & Technology, College of Agriculture, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran
Correspondence
Mahsa Shojaei and Mohammadreza Eshaghi, Department of Food Science & Technology, College of Agriculture, Varamin - Pishva branch, Islamic Azad University, Varamin, Iran.
Email: [email protected], [email protected]
Search for more papers by this authorLeila Nateghi
Department of Food Science & Technology, College of Agriculture, Varamin - Pishva Branch, Islamic Azad University, Varamin, Iran
Search for more papers by this authorAbstract
In the current study, some physicochemical, mechanical, thermal, structural, and antibacterial properties of hydroxypropyl methyl cellulose–whey protein concentrate (HPMC–WPC) film loaded with chitosan nanoparticles (CNPs) are scrutinized. Initially, the optimal ratio of HPMC:WPC (70:30) was screened, then CNPs loaded into the HPMC:WPC blend film at the levels of 1%–5%. Well compatibility of the blend film components and generation intermolecular interactions were demonstrated by Fourier transform infrared and X-ray diffraction tests, respectively. A uniform distribution of CNPs within the film structure was monitored by scanning electron microscopy micrographs. The DSC scans showed an upgrading in thermal characterizations of the blend film as a result of CNPs addition. As well, the water vapor permeability and flexibility of bionanocomposites decreased, while the tensile strength improved considerably (p < .05). The antibacterial effects of CNPs was more evident on Gram-positive (Listeria monocytogenes and Staphylococcus aureus) rather than Gram-negative (Escherichia coli) bacteria.
Practical applications
The current research would have various uses in the area of food packaging. Bio-based packaging films are developed to substitute petrol-based materials for health and ecological reasons. Nanoparticle loading and blending of plant-based biopolymer films are strategies can strength their physical, mechanical, structural, and antibacterial attributes.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
REFERENCES
- Akhtar, M. J., Jacquot, M., Jasniewski, J., Jacquot, C., Imran, M., Jamshidian, M., … Desobry, S. (2012). Antioxidant capacity and light-aging study of HPMC films functionalized with natural plant extract. Carbohydrate Polymers, 89(4), 1150–1158. https://doi.org/10.1016/j.carbpol.2012.03.088
- Alboofetileh, M., Rezaei, M., Hosseini, H., & Abdollahi, M. (2014). Antimicrobial activity of alginate/clay nanocomposite films enriched with essential oils against three common foodborne pathogens. Food Control, 36(1), 1–7. https://doi.org/10.1016/j.foodcont.2013.07.037
- Antoniou, J., Liu, F., Majeed, H., & Zhong, F. (2015). Characterization of tara gum edible films incorporated with bulk chitosan and chitosan nanoparticles: A comparative study. Food Hydrocolloids, 44, 309–319. https://doi.org/10.1016/j.foodhyd.2014.09.023
- Bahram, S., Rezaei, M., Soltani, M., Kamali, A., Ojagh, S. M., & Abdollahi, M. (2014). Whey protein concentrate edible film activated with cinnamon essential oil. Journal of Food Processing and Preservation, 38(3), 1251–1258. https://doi.org/10.1111/jfpp.12086
- Bahrami, A., Mokarram, R. R., Khiabani, M. S., Ghanbarzadeh, B., & Salehi, R. (2018). Physico-mechanical and antimicrobial properties of tragacanth/hydroxypropyl methylcellulose/beeswax edible films reinforced with silver nanoparticles. International Journal of Biological Macromolecules, 129, 1103–1112. https://doi.org/10.1016/j.ijbiomac.2018.09.045
- Bao, S., Xu, S., & Wang, Z. (2009). Antioxidant activity and properties of gelatin films incorporated with tea polyphenol-loaded chitosan nanoparticles. Journal of the Science of Food and Agriculture, 89(15), 2692–2700.
- Bilbao-Sáinz, C., Avena-Bustillos, R. J., Wood, D. F., Williams, T. G., & McHugh, T. H. (2010). Composite edible films based on hydroxypropyl methylcellulose reinforced with microcrystalline cellulose nanoparticles. Journal of Agricultural and Food Chemistry, 58(6), 3753–3760. https://doi.org/10.1021/jf9033128
- Bourbon, A. I., Pinheiro, A. C., Cerqueira, M. A., Rocha, C. M., Avides, M. C., Quintas, M. A., & Vicente, A. A. (2011). Physico-chemical characterization of chitosan-based edible films incorporating bioactive compounds of different molecular weight. Journal of Food Engineering, 106(2), 111–118. https://doi.org/10.1016/j.jfoodeng.2011.03.024
- Chang, P. R., Jian, R., Yu, J., & Ma, X. (2010). Fabrication and characterisation of chitosan nanoparticles/plasticised-starch composites. Food Chemistry, 120(3), 736–740. https://doi.org/10.1016/j.foodchem.2009.11.002
- Chen, L., & Subirade, M. (2009). Elaboration and characterization of soy/zein protein microspheres for controlled nutraceutical delivery. Biomacromolecules, 10(12), 3327–3334. https://doi.org/10.1021/bm900989y
- de Moura, M. R., Avena-Bustillos, R. J., McHugh, T. H., Krochta, J. M., & Mattoso, L. H. C. (2008). Properties of novel hydroxypropyl methylcellulose films containing chitosan nanoparticles. Journal of Food Science, 73(7), N31–N37. https://doi.org/10.1111/j.1750-3841.2008.00872.x
- Dehnad, D., Emam-Djomeh, Z., Mirzaei, H., Jafari, S. M., & Dadashi, S. (2014). Optimization of physical and mechanical properties for chitosan–nanocellulose biocomposites. Carbohydrate Polymers, 105, 222–228. https://doi.org/10.1016/j.carbpol.2014.01.094
- Dehnad, D., Mirzaei, H., Emam-Djomeh, Z., Jafari, S. M., & Dadashi, S. (2014). Thermal and antimicrobial properties of chitosan–nanocellulose films for extending shelf life of ground meat. Carbohydrate Polymers, 109, 148–154. https://doi.org/10.1016/j.carbpol.2014.03.063
- Ding, C., Zhang, M., & Li, G. (2015). Preparation and characterization of collagen/hydroxypropyl methylcellulose (HPMC) blend film. Carbohydrate Polymers, 119, 194–201. https://doi.org/10.1016/j.carbpol.2014.11.057
- Divya, K., Vijayan, S., George, T. K., & Jisha, M. S. (2017). Antimicrobial properties of chitosan nanoparticles: Mode of action and factors affecting activity. Fibers and Polymers, 18(2), 221–230. https://doi.org/10.1007/s12221-017-6690-1
- Galus, S., & Kadzińska, J. (2016). Whey protein edible films modified with almond and walnut oils. Food Hydrocolloids, 52, 78–86. https://doi.org/10.1016/j.foodhyd.2015.06.013
- Garavand, F., & Madadlou, A. (2014). Recovery of phenolic compounds from effluents by a microemulsion liquid membrane (MLM) extractor. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 443, 303–310. https://doi.org/10.1016/j.colsurfa.2013.11.035
- Garavand, F., Rouhi, M., Razavi, S. H., Cacciotti, I., & Mohammadi, R. (2017). Improving the integrity of natural biopolymer films used in food packaging by crosslinking approach: A review. International Journal of Biological Macromolecules, 104, 687–707. https://doi.org/10.1016/j.ijbiomac.2017.06.093
- Hosseini, S. F., Rezaei, M., Zandi, M., & Farahmandghavi, F. (2015). Fabrication of bio-nanocomposite films based on fish gelatin reinforced with chitosan nanoparticles. Food Hydrocolloids, 44, 172–182. https://doi.org/10.1016/j.foodhyd.2014.09.004
- Hosseini, S. F., Rezaei, M., Zandi, M., & Farahmandghavi, F. (2016). Development of bioactive fish gelatin/chitosan nanoparticles composite films with antimicrobial properties. Food Chemistry, 194, 1266–1274. https://doi.org/10.1016/j.foodchem.2015.09.004
- Hosseinnejad, M., & Jafari, S. M. (2016). Evaluation of different factors affecting antimicrobial properties of chitosan. International Journal of Biological Macromolecules, 85, 467–475. https://doi.org/10.1016/j.ijbiomac.2016.01.022
- Jafari, S. M., Khanzadi, M., Mirzaei, H., Dehnad, D., Chegini, F. K., & Maghsoudlou, Y. (2015). Hydrophobicity, thermal and micro-structural properties of whey protein concentrate–pullulan–beeswax films. International Journal of Biological Macromolecules, 80, 506–511. https://doi.org/10.1016/j.ijbiomac.2015.07.017
- Khanzadi, M., Jafari, S. M., Mirzaei, H., Chegini, F. K., Maghsoudlou, Y., & Dehnad, D. (2015). Physical and mechanical properties in biodegradable films of whey protein concentrate–pullulan by application of beeswax. Carbohydrate Polymers, 118, 24–29. https://doi.org/10.1016/j.carbpol.2014.11.015
- Kong, M., Chen, X. G., Xing, K., & Park, H. J. (2010). Antimicrobial properties of chitosan and mode of action: A state of the art review. International Journal of Food Microbiology, 144(1), 51–63. https://doi.org/10.1016/j.ijfoodmicro.2010.09.012
- Kraisit, P., Limmatvapirat, S., Nunthanid, J., Sriamornsak, P., & Luangtana-Anan, M. (2016). Preparation and characterization of hydroxypropyl methylcellulose/polycarbophil mucoadhesive blend films using a mixture design approach. Chemical and Pharmaceutical Bulletin, 65(3), 284–294. https://doi.org/10.1248/cpb.c16-0084
- Kurek, M., Galus, S., & Debeaufort, F. (2014). Surface, mechanical and barrier properties of bio-based composite films based on chitosan and whey protein. Food Packaging and Shelf Life, 1(1), 56–67. https://doi.org/10.1016/j.fpsl.2014.01.001
10.1016/j.fpsl.2014.01.001 Google Scholar
- Le Tien, C., Letendre, M., Ispas-Szabo, P., Mateescu, M. A., Delmas-Patterson, G., Yu, H. L., & Lacroix, M. (2000). Development of biodegradable films from whey proteins by cross-linking and entrapment in cellulose. Journal of Agricultural and Food Chemistry, 48(11), 5566–5575. https://doi.org/10.1021/jf0002241
- Li, C., Zhu, W., Xue, H., Chen, Z., Chen, Y., & Wang, X. (2015). Physical and structural properties of peanut protein isolate-gum Arabic films prepared by various glycation time. Food Hydrocolloids, 43, 322–328. https://doi.org/10.1016/j.foodhyd.2014.06.003
- Liu, L., Lin, W. J., Liu, H. Z., Shi, A. M., Hu, H., Nasir, M. N., … Wang, Q. (2017). Effect of xylose on the structural and physicochemical properties of peanut isolated protein based films. RSC Advances, 7(83), 52357–52365. https://doi.org/10.1039/C7RA07381J
- Liu, M., Jia, Z., Liu, F., Jia, D., & Guo, B. (2010). Tailoring the wettability of polypropylene surfaces with halloysite nanotubes. Journal of Colloid and Interface Science, 350(1), 186–193. https://doi.org/10.1016/j.jcis.2010.06.047
- Lorevice, M. V., Otoni, C. G., de Moura, M. R., & Mattoso, L. H. C. (2016). Chitosan nanoparticles on the improvement of thermal, barrier, and mechanical properties of high-and low-methyl pectin films. Food Hydrocolloids, 52, 732–740. https://doi.org/10.1016/j.foodhyd.2015.08.003
- Martelli, M. R., Barros, T. T., de Moura, M. R., Mattoso, L. H., & Assis, O. B. (2013). Effect of chitosan nanoparticles and pectin content on mechanical properties and water vapor permeability of banana puree films. Journal of Food Science, 78(1), 98–104. https://doi.org/10.1111/j.1750-3841.2012.03006.x
- Mirzaei-Mohkam, A., Garavand, F., Dehnad, D., Keramat, J., & Nasirpour, A. (2019). Optimisation, antioxidant attributes, stability and release behaviour of carboxymethyl cellulose films incorporated with nanoencapsulated vitamin E. Progress in Organic Coatings, 134, 333–341. https://doi.org/10.1016/j.porgcoat.2019.05.026.
- Mohammadpour Dounighi, N., Eskandari, R., Avadi, M. R., Zolfagharian, H., Mir Mohammad Sadeghi, A., & Rezayat, M. (2012). Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system. Journal of Venomous Animals and Toxins including Tropical Diseases, 18(1), 44–52. https://doi.org/10.1590/S1678-91992012000100006
- Nooshkam, M., & Madadlou, A. (2016). Microwave-assisted isomerisation of lactose to lactulose and Maillard conjugation of lactulose and lactose with whey proteins and peptides. Food Chemistry, 200, 1–9. https://doi.org/10.1016/j.foodchem.2015.12.094
- Noshirvani, N., Ghanbarzadeh, B., Mokarram, R. R., Hashemi, M., & Coma, V. (2017). Preparation and characterization of active emulsified films based on chitosan-carboxymethyl cellulose containing zinc oxide nano particles. International Journal of Biological Macromolecules, 99, 530–538. https://doi.org/10.1016/j.ijbiomac.2017.03.007
- Priya, D. S., Suriyaprabha, R., Yuvakkumar, R., & Rajendran, V. (2014). Chitosan-incorporated different nanocomposite HPMC films for food preservation. Journal of Nanoparticle Research, 16(2), 2248. https://doi.org/10.1007/s11051-014-2248-y
- Sebti, I., Chollet, E., Degraeve, P., Noel, C., & Peyrol, E. (2007). Water sensitivity, antimicrobial, and physicochemical analyses of edible films based on HPMC and/or chitosan. Journal of Agricultural and Food Chemistry, 55(3), 693–699. https://doi.org/10.1021/jf062013n
- Su, J. F., Huang, Z., Yuan, X. Y., Wang, X. Y., & Li, M. (2010). Structure and properties of carboxymethyl cellulose/soy protein isolate blend edible films crosslinked by Maillard reactions. Carbohydrate Polymers, 79(1), 145–153. https://doi.org/10.1016/j.carbpol.2009.07.035
- Su, J. F., Yuan, X. Y., Huang, Z., Wang, X. Y., Lu, X. Z., Zhang, L. D., & Wang, S. B. (2012). Physicochemical properties of soy protein isolate/carboxymethyl cellulose blend films crosslinked by Maillard reactions: Color, transparency and heat-sealing ability. Materials Science and Engineering: C, 32(1), 40–46. https://doi.org/10.1016/j.msec.2011.09.009
- Sukhija, S., Singh, S., & Riar, C. S. (2016). Analyzing the effect of whey protein concentrate and psyllium husk on various characteristics of biodegradable film from lotus (Nelumbo nucifera) rhizome starch. Food Hydrocolloids, 60, 128–137. https://doi.org/10.1016/j.foodhyd.2016.03.023
- Tabatabaei, R. H., Jafari, S. M., Mirzaei, H., Nafchi, A. M., & Dehnad, D. (2018). Preparation and characterization of nano-SiO2 reinforced gelatin-k-carrageenan biocomposites. International Journal of Biological Macromolecules, 111, 1091–1099. https://doi.org/10.1016/j.ijbiomac.2018.01.116
- Tian, H., Xu, G., Yang, B., & Guo, G. (2011). Microstructure and mechanical properties of soy protein/agar blend films: Effect of composition and processing methods. Journal of Food Engineering, 107(1), 21–26. https://doi.org/10.1016/j.jfoodeng.2011.06.008
- Vahedikia, N., Garavand, F., Tajeddin, B., Cacciotti, I., Jafari, S. M., Omidi, T., & Zahedi, Z. (2019). Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: Physical, mechanical, structural and antimicrobial attributes. Colloids and Surfaces B: Biointerfaces, 177, 25–32. https://doi.org/10.1016/j.colsurfb.2019.01.045
- Vatankhah, M., Garavand, F., Mohammadi, B., & Elhamirad, A. (2017). Quality attributes of reduced-sugar Iranian traditional sweet bread containing stevioside. Journal of Food Measurement and Characterization, 11(3), 1233–1239. https://doi.org/10.1007/s11694-017-9500-y
- Wang, L., Xiao, M., Dai, S., Song, J., Ni, X., Fang, Y., … Jiang, F. (2014). Interactions between carboxymethyl konjac glucomannan and soy protein isolate in blended films. Carbohydrate Polymers, 101, 136–145. https://doi.org/10.1016/j.carbpol.2013.09.028
- Wardani, G., M, M., & Sudjarwo, S. A. (2018). In vitro antibacterial activity of chitosan nanoparticles against Mycobacterium tuberculosis. Pharmacognosy Journal, 10(1), 162–166. https://doi.org/10.5530/pj.2018.1.27
- Xue, F., Li, C., Zhu, X., Wang, L., & Pan, S. (2013). Comparative studies on the physicochemical properties of soy protein isolate-maltodextrin and soy protein isolate-gum acacia conjugate prepared through Maillard reaction. Food Research International, 51(2), 490–495. https://doi.org/10.1016/j.foodres.2013.01.012