Intraoperative detection of IDH-mutant glioma using fluorescence lifetime imaging
Silvia Noble Anbunesan
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorAlba Alfonso-Garcia
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorXiangnan Zhou
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorJulien Bec
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorHan Sung Lee
Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, California, USA
Search for more papers by this authorLee-Way Jin
Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, California, USA
Search for more papers by this authorOrin Bloch
Department of Neurological Surgery, University of California Davis, Sacramento, California, USA
Search for more papers by this authorCorresponding Author
Laura Marcu
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Department of Neurological Surgery, University of California Davis, Sacramento, California, USA
Correspondence
Laura Marcu, Department of Biomedical Engineering, University of California Davis, Davis, CA, USA.
Email: [email protected]
Search for more papers by this authorSilvia Noble Anbunesan
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorAlba Alfonso-Garcia
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorXiangnan Zhou
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorJulien Bec
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Search for more papers by this authorHan Sung Lee
Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, California, USA
Search for more papers by this authorLee-Way Jin
Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, California, USA
Search for more papers by this authorOrin Bloch
Department of Neurological Surgery, University of California Davis, Sacramento, California, USA
Search for more papers by this authorCorresponding Author
Laura Marcu
Department of Biomedical Engineering, University of California Davis, Davis, California, USA
Department of Neurological Surgery, University of California Davis, Sacramento, California, USA
Correspondence
Laura Marcu, Department of Biomedical Engineering, University of California Davis, Davis, CA, USA.
Email: [email protected]
Search for more papers by this authorAbstract
Identifying isocitrate dehydrogenase (IDH)-mutation and glioma subtype during surgery instead of days later can aid in modifying tumor resection strategies for better survival outcomes. We report intraoperative identification of IDH-mutant glioma (N = 12 patients) with a clinically compatible fluorescence lifetime imaging (FLIm) device (excitation: 355 nm; emission spectral bands: 390/40 nm, 470/28 nm, 542/50 nm). The fluorescence-derived parameters were analyzed to study the optical contrast between IDH-mutant tumors and surrounding brain tissue. IDH-mutant oligodendrogliomas exhibited shorter lifetimes (3.3 ± 0.1 ns) than IDH-mutant astrocytomas (4.1 ± 0.1 ns). Both IDH-mutant glioma subtypes had shorter lifetimes than white matter (4.6 ± 0.4 ns) but had comparable lifetimes to cortex. Lifetimes also increased with malignancy grade within IDH-mutant oligodendrogliomas (grade 2: 2.96 ± 0.08 ns, grade 3: 3.4 ± 0.3 ns) but not within IDH-mutant astrocytomas. The current results support the feasibility of FLIm as a surgical adjuvant for identifying IDH-mutant glioma tissue.
CONFLICT OF INTEREST
The authors declare no financial or commercial conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| jbio202200291-sup-0001-FigureS1.tiffTIFF image, 11.7 MB | Figure S1. H&E stains and corresponding IDH stains for different classes of tumor cellular density from tumor tissue embedded in white matter. All images were acquired at ×40 magnification. |
| jbio202200291-sup-0002-FigureS2.tiffTIFF image, 1.2 MB | Figure S2. Average lifetime at 470-nm and 542-nm from individual point measurements from scans acquired in (A) white matter (green) vs. tumor in astrocytoma (teal), (B) cortex (blue) in astrocytoma, (C) white matter (green) vs. tumor in oligodendroglioma (orange), and (D) cortex (blue) vs. tumor in oligodendroglioma (orange). N indicates number of patients; n indicates number of scans; P indicates the number of point measurements. |
| jbio202200291-sup-0003-FigureS3.tiffTIFF image, 483.4 KB | Figure S3. Average lifetimes of white matter tissue with different cellular densities (absent, moderate, low, and dense) for each patient across all spectral bands. Patients indicated by * include data collected from system S1. |
| jbio202200291-sup-0004-supinfo.docxWord 2007 document , 1.6 MB | Data S1. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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