Development of a pea protein-/chitosan-based bioactive film using Aronia melanocarpa polyphenols as a bioactive ingredient
Xijun Nan
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Visualization, Writing - original draft
Search for more papers by this authorQuancheng Zhou
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorWei Ji
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing - original draft
Search for more papers by this authorXuanhong Chen
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorJiayi Li
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorHonglei Wang
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorLeichao Dong
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorXue Meng
Zibo Forestry Protection and Development Center, Zibo, China
Contribution: Resources
Search for more papers by this authorCorresponding Author
Guihua Sheng
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Correspondence
Guihua Sheng, Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zhangdian District, Zibo, Shandong province, China.
Email: [email protected]
Contribution: Data curation, Funding acquisition, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorXijun Nan
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Validation, Visualization, Writing - original draft
Search for more papers by this authorQuancheng Zhou
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorWei Ji
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing - original draft
Search for more papers by this authorXuanhong Chen
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorJiayi Li
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorHonglei Wang
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorLeichao Dong
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Contribution: Data curation, Formal analysis, Investigation, Methodology, Visualization
Search for more papers by this authorXue Meng
Zibo Forestry Protection and Development Center, Zibo, China
Contribution: Resources
Search for more papers by this authorCorresponding Author
Guihua Sheng
Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China
Correspondence
Guihua Sheng, Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zhangdian District, Zibo, Shandong province, China.
Email: [email protected]
Contribution: Data curation, Funding acquisition, Project administration, Supervision, Writing - review & editing
Search for more papers by this authorAbstract
The study was designed to develop a pea protein- (PP) and chitosan (CS)-based bioactive composite film using purified Aronia melanocarpa polyphenols (AMP) as a bioactive ingredient (PP-CS-AMP). First, AMP was extracted and purified, and the antioxidant properties of AMP were tested, and the result indicated that AMP showed stronger antioxidant properties than vitamin C (Vc). Second, five kinds of PP-CS film were developed: PP including unextruded pea protein (NEP), extrusion pea protein (EP), papain co-extruded pea protein (PEP), neutral protease co-extruded pea protein (NPEP), and double enzyme combined extrusion pea protein (DCEP). The five kinds of PP-CS film were characterized and evaluated for various functions and properties; principal component analysis and membership evaluation were used to analyze the comprehensive scores of the films. It was observed that the NPEP-CS film obtained the highest score. Extrusion and enzyme extrusion treatments improved the structure and properties of PP, and therefore enhanced the properties of PP-CS film. Finally, NPEP-CS-AMP film was developed (AMP content 0, 4.0% and 8.0%, w/v) and tested for their performance, and the results suggested that the addition of 4.0% AMP gives the NPEP-CS-AMP film excellent physical and antioxidant properties.
Novelty impact statement
- Aronia melanocarpa polyphenols (AMP), as a bioactivity ingredient was firstly incorporated into pea protein (PP) - chitosan (CS) film.
- Extrusion and enzyme extrusion can improve the structure and properties of PP, which is beneficial to improvement on the properties of PP-CS film.
- NPEP-CS-AMP film exhibit excellent physical properties, structural performance and high antioxidant activity.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
Open Research
DATA AVAILABILITY STATEMENT
Data sharing not applicable – no new data generated, or the article describes entirely theoretical research.
REFERENCES
- Aruchida, K., Thanaporn, S., & Nathdanai, H. (2020). Active meat packaging from thermoplastic cassava starch containing sappan and cinnamon herbal extracts via lldpe blown-film extrusion. Food Packaging and Shelf Life, 26, 100557.
- Assad, I., Bhat, S. U., Gani, A., & Shah, A. (2020). Protein based packaging of plant origin: Fabrication, properties, recent advances and future perspectives. International Journal of Biological Macromolecules, 164, 707–716.
- Babaei, J., Mohammadian, M., & Madadlou, A. (2019). Gelatin as texture modifier and porogen in egg white hydrogel. Food Chemistry, 270, 189–195.
- Bhat, Z. F., & Pathak, V. (2009). Effect of mung bean (Vigna radiata) on quality characteristics of oven roasted chicken seekh kababs. Fleischwirtschaft International, 6, 58–60.
- Bi, F., Zhang, X., Bai, R., Liu, Y., & Liu, J. (2019). Preparation and characterization of antioxidant and antimicrobial packaging films based on chitosan and proanthocyanidins. International Journal of Biological Macromolecules, 134, 11–19.
- Brink, I., Šipailienė, A., & Leskauskaitė, D. (2019). Antimicrobial properties of chitosan and whey protein films applied on fresh cut Turkey pieces. International Journal of Biological Macromolecules, 130, 810–817.
- Chen, S. S., Tao, H. J., Wang, Y. J., & Ma, Z. S. (2016). Process optimization of soy protein isolate-based edible films containing nanocrystalline cellulose from sunflower seed hull and chitosan. Transactions of the Chinese Society of Agricultural Engineering, 32(8), 306–314.
- Cong, L. J., Shi, R., Wu, P., Liu, M. M., Liu, S. W., & Huang, X. T. (2021). Content determination of total polyphenols and total flavones in the fruits of Aronia melanocarpa from different producing areas. Journal of Liaoning University of Traditional Chinese Medicine, 201(1), 37–40.
- Dong, L. C., Chen, X. H., & Wang, S. (2020). Effect of potato starch on structure and properties of pea protein 3D printing materials. Chinese Journal of Food Science and Technology, 20(1), 127–133.
- Dua, S., Bhat, Z. F., & Kumar, S. (2015). Effect of oleuropein on the oxidative stability and storage quality of Tabaq-Maz, fried mutton ribs. Food Bioscience, 12, 84–92.
- Emma, T., Kata, T. T., Vikto, A. N., Branko, M. B., Maria, V., Amparo, C., & Chelo, G. M. (2017). Antioxidant edible films based on chitosan and starch containing polyphenols from thyme extracts. Carbohydrate Polymers, 157, 1153–1161.
- Estevez-Areco, S., Guz, L., Fama, L., Candal, R., & Goyanes, S. (2019). Bioactive starch nanocomposite films with antioxidant activity and enhanced mechanical properties obtained by extrusion followed by thermo-compression. Food Hydrocolloids, 96, 518–528.
- Friesen, K., Chang, C., & Nickerson, M. (2015). Incorporation of phenolic compounds, rutin and epicatechin, into soy protein isolate films: Mechanical, barrier and cross-linking properties - sciencedirect. Food Chemistry, 172, 18–23.
- Gao, N. X. (2017). Extraction and purification process of polyphenols from Liriodendron melanocarpa and its antioxidant activity and stability. Shenyang Agricultural University.
- Gao, N. X., Li, B., Du, S. S., Meng, X. J., Zhang, Q., Jiao, X. Y., & Chen, S. F. (2016). Stability of polyphenols from Aronia melanocarpa fruits. Food Science, 37, 5.
- Ge, J., Sun, C. X., & Harold, C. (2020). The health benefits, functional properties, modifications, and applications of pea (Pisum sativum L.) protein: Current status, challenges, and perspectives. Comprehensive Reviews in Food Science and Food Safety, 19(4), 1–42.
- Gutierrez, T. J., Toro-Marquez, L. A., Merino, D., & Mendieta, J. R. (2019). Hydrogen-bonding interactions and compostability of bionano- composite films prepared from corn starch and nano-fillers with and without added Jamaica flower extract. Food Hydrocolloids, 89, 283–293.
- Halasz, K., & Csoka, L. (2018). Extract immobilized from black chokeberry (Aronia melanocarpa) prawn indicator film. Food Packaging and Shelf Life, 16, 185–193.
- Han, J. H. (2014). A review of food packaging technologies and innovations. Innovations in Food Packaging, 1, 3–12.
10.1016/B978-0-12-394601-0.00001-1 Google Scholar
- Hao, L., Na, J., & Man, L. (2019). Preparation of borax cross-linked starch nanoparticles for improvement of mechanical properties of maize starch films. Journal of Agricultural and Food Chemistry, 67, 2916–2925.
- Ieva, B., Aušra, Š., & Daiva, L. (2019). Antimicrobial properties of chitosan and whey protein films applied on fresh cut Turkey pieces. International Journal of Biological Macromolecules, 130, 810–817.
- Jamwal, A., Kumar, S., Bhat, Z. F., Kumar, A., & Kaur, S. (2015). The quality and storage stability of chicken patties prepared with different additives. Nutrition and Food Science, 45, 728–739.
- Jancikova, S., Jamróz, E., Kulawik, P., Tkaczewska, J., & Dordevic, D. (2019). Furcellaran/gelatin hydrolysate/rosemary extract composite films as active and intelligent packaging materials. International Journal of Biological Macromolecules, 131, 19–28.
- Jridi, M., Abdelhedi, O., & Zouari, N. (2018). Development and characterization of grey triggerfish gelatin/agar bilayer and blend films containing vine leaves bioactive compounds. Food Hydrocolloids, 89, 1–39.
- Kalem, I. K., Bhat, Z. F., Kumar, S., Noor, S., & Desai, A. (2018). The effects of bioactive edible film containing Terminalia arjuna on the stability of some quality attributes of chevon sausage. Meat Science, 140, 38–43.
- Kalkan, S., Ota, M. R., & Engin, M. S. (2020). Physicochemical and bioactive properties of edible methylcellulose films containing rheum ribes l. extract - sciencedirect. Food Chemistry, 307, 125524.
- Kumar, P., Singh, A., Kumar, S., & Bhat, Z. F. (2015). Effect of processed oats and clove oil on the characteristics and storage quality of aerobically packaged chevon cutlets. Indian Journal of Small Ruminants, 21, 76–84.
10.5958/0973-9718.2015.00009.4 Google Scholar
- Kumar, S., Bhat, Z. F., & Kumar, P. (2011). Effect of apple pulp and Celosia argentea on the quality characteristics of Shrikhand. American Journal of Food Technology, 6(9), 1–8.
10.3923/ajft.2011.817.826 Google Scholar
- Lee, J. Y., Garcia, C. V., Shin, G. H., & Kim, J. T. (2019). Antibacterial and antioxidant properties of hydroxypropyl methylcellulose-based active composite films incorporating oregano essential oil nanoemulsions. LWT - Food Science and Technology, 106, 164–171.
- Li, B., Gao, N. X., & Liu, H. (2016). Optimization of purification process of polyphenols from Liriodendron liriodendron by macroporous resin. Food Science, 37(16), 69–74.
- Li, H. C., & Yang, S. S. (2019). Preparation and characterization of gelatin/chitosan composite membrane. New Chemical Materials, 47(2), 228–231.
- Li, X., Chen, W. X., & Wang, C. J. (2019). Chitosan and nano-TiO2. Science and Technology of Food Industry, 40(18), 212–216, 254 .
- Liang, T., Sun, G., Cao, L., Li, J., & Wang, L. (2018). Rheological behavior of film-forming solutions and film properties from Artemisia sphaerocephala krasch. Gum and purple onion peel extract. Food Hydrocolloids, 82, 124–134.
- Liu, F., Avena-Bustillos, R. J., Chiou, B. S., Li, Y., Ma, Y., Williams, T. G., Wood, D. F., McHugh, T. H., & Zhong, F. (2017). Controlled-release of tea polyphenol from gelatin films incorporated with different ratios of free/nanoencapsulated tea polyphenols into fatty food simulants. Food Hydrocolloids, 62, 212–221.
- Liu, F. G., Ma, C. C., & Wang, D. (2016). Research progress on interaction between protein and polyphenols. Food & Fermentation Industry, 42(2), 282–288.
- Liu, X. X., Wang, J. J., Liu, H. L., & Fan, C. Y. (2012). Preparation and characterization of chitosan/soy protein isolate packaging composite film. Packaging Engineering, 3, 46–50.
- Luo, A. G., Zhao, Q., Ma, J. H., Yang, Y. J., & Hu, B. F. (2020). Preparation and characterization of phycocyanin and chitosan composite membrane. Science and Technology of Food Industry, 41(23), 33–37 44.
- Lza, B., Dya, B., Jmrb, C., Wxa, B., & Jda, B. (2021). A comprehensive review on natural bioactive films with controlled release characteristics and their applications in foods and pharmaceuticals. Trends in Food Science & Technology, 112, 690–707.
- Maryam, M., Maryam, S., Mehdi, M., Maryam, K., & Zahra, E. D. (2020). Development of antioxidant edible films based on mung bean protein enriched with pomegranate peel. Food Hydrocolloids, 104, 105735.
- Narasagoudr, S. S., Hegde, V. G., Chougale, R. B., Masti, S. P., & Malabadi, R. B. (2020). Physico-chemical and functional properties of rutin induced chitosan/poly (vinyl alcohol) bioactive films for food packaging applications. Food Hydrocolloids, 109, 106096.
- Othman, S. H., & Rashid, S. A. (2020). Effects of glycol and thymol on thermal properties of corn starch films. Food Hydrocolloids, 106, 105884.
- Patricia, C., Gonzalo, V., & Manuel, V. (2019). Novel composite films from regenerated cellulose-glycerol-polyvinyl alcohol: Mechanical and barrier properties. Food Hydrocolloids, 89, 481–491.
- Qin, Y., Liu, Y., Yuan, L., Yong, H., & Liu, J. (2019). Preparation and characterization of antioxidant, antimicrobial and ph-sensitive films based on chitosan, silver nanoparticles and purple corn extract. Food Hydrocolloids, 96, 102–111.
- Qin, Y., Liu, Y., Zhang, X., & Liu, J. (2020). Development of active and intelligent packaging by incorporating betalains from red pitaya (Hylocereus polyrhizus) peel into starch/polyvinyl alcohol films. Food Hydrocolloids, 100, 105410.
- Rao, X. T., Zeng, X. A., Lin, S. Y., Liu, Z. H., & Sun, J. J. (2020). Changing trends in the phenolic substances and antioxidant activities of chokeberry (Aronia melanocarpa) subjected to in vitro simulated digestion. Modern Food Science and Technology, 12, 78–83.
- Saroat, R., Aryna, F., Soottawat, B., & Pimonpan, K. (2020). Application of anthocyanin as a color indicator in gelatin films. Food Bioscience, 36, 100603.
- Singh, P. K., Kumar, S., Bhat, Z. F., & Kumar, P. (2015). Effect of sorghum bicolour and clove oil on the quality characteristics and storage quality of aerobically packaged chevon cutlets. Nutrition and Food Science, 45, 145–163.
- Singh, T. P., Agrawal, R. K., Mendiratta, S. K., & Chauhan, G. (2021). Preparation and characterization of licorice root extract infused bio-composite film and their application on storage stability of chhana balls-a Sandesh like product. Food Control, 125, 107993.
- Song, Y., & Yoo, S. H. (2017). Quality improvement of a rice-substituted fried noodle by utilizing the protein-polyphenol interaction between a pea protein isolate and green tea (Camellia sinensis) extract. Food Chemistry, 235(15), 181–187.
- Sun, J. J. (2017). Study on properties of polyphenol chitosan composite membrane of apple fruit and its effect on fish preservation. Shaanxi Normal University, 1, 5–53.
- Sun, H. T., Nshao, X. R., & Jiang, R. P. (2016). Preparation and physical properties of corn distarch phosphate/corn straw cellulose edible film. Food Science, 37, 21–28.
- Sun, J., Jiang, H., & Wu, H. (2020). Multifunctional bionanocomposite films based on konjac glucomannan/chitosan with nano-ZnO and mulberry anthocyanin extract for active food packaging. Food Hydrocolloids, 107, 105942.
- Tainara, D., Alessandro, D., Alves, V., & Bandarra, N. (2018). Biodegradable films based on gelatin and papaya peel microparticles with antioxidant properties. Food & Bioprocess Technology, 11(3), 536–550.
- Wang, M., Chen, X. H., Dong, L. C., Nan, X. J., Ji, W., Wang, S., Sun, W. T., & Zhou, Q. C. (2021). Modification of pea dietary fiber by ultrafine grinding and hypoglycemic effect in diabetes mellitus mice. Journal of Food Science, 86, 1273–1282.
- Wang, Q. M., Tang, Y. W., & Yang, Y. X. (2019). Research progress of plant polyphenol-protein composite antibacterial membrane. Food & Fermentation Industry, 45(10), 247–252.
- Wang, X., Yong, H., Gao, L., Li, L., Jin, M., & Liu, J. (2019). Preparation and characterization of antioxidant and pH-sensitive films based on chitosan and black soybean seed coat extract. Food Hydrocolloids, 89, 56–66.
- Wu, H. J., Lei, Y. L., Zhu, R., Zhao, M. J., & Lu, J. Y. (2019). Preparation and characterization of bioactive edible packaging films based on pomelo peel flours incorporating tea polyphenol - sciencedirect. Food Hydrocolloids, 90, 41–49.
- Xu, Y., Wang, C., Wang, P., & Xiao, Z. G. (2017). Study on preparation and properties of extruded rice protein-glucose conjugates. Cereals & Oils, 4, 85–89.
- Yang, H., Wang, J., Yang, F., Chen, M., Zhou, D., & Li, L. (2016). Active packaging films from ethylene vinyl alcohol copolymer and clove essential oil as shelf life extenders for grass carp slice. Packaging Technology & Science, 29(7), 383–396.
- Yin, T. T. (2019). Preparation of soybean protein crosslinked corn starch/Nano-TiO2 packaging film and application of cherry tomatoes preservation. Northeast Agricultural University.
- Zhang, X., Liu, Y., Yong, H., Qin, Y., Liu, J., & Liu, J. (2019). Development of multifunctional food packaging films based on chitosan, TiO2 nanoparticles and anthocyanin-rich black plum peel extract. Food Hydrocolloids, 94, 80–92.
- Zhou, Q. C., Liu, N., & Feng, C. X. (2017). Research on the effect of papain co-extrusion on pea protein and Enzymolysis antioxidant peptides. Journal of Food Processing and Preservation, 41(6), 13301.
- Zhu, F. (2021). Polysaccharide based films and coatings for food packaging: Effect of added polyphenols. Food Chemistry, 359, 129871.