Volume 46, Issue 12 e17168
ORIGINAL ARTICLE

Development of a pea protein-/chitosan-based bioactive film using Aronia melanocarpa polyphenols as a bioactive ingredient

Xijun Nan

Xijun Nan

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Project administration, Validation, Visualization, Writing - original draft

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Quancheng Zhou

Quancheng Zhou

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Data curation, Project administration, Supervision, Writing - review & editing

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Wei Ji

Wei Ji

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Validation, Visualization, Writing - original draft

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Xuanhong Chen

Xuanhong Chen

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Visualization

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Jiayi Li

Jiayi Li

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Visualization

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Honglei Wang

Honglei Wang

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Visualization

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Leichao Dong

Leichao Dong

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Contribution: Data curation, Formal analysis, ​Investigation, Methodology, Visualization

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Xue Meng

Xue Meng

Zibo Forestry Protection and Development Center, Zibo, China

Contribution: Resources

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Guihua Sheng

Corresponding Author

Guihua Sheng

Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

Correspondence

Guihua Sheng, Department of Food Science, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zhangdian District, Zibo, Shandong province, China.

Email: [email protected]

Contribution: Data curation, Funding acquisition, Project administration, Supervision, Writing - review & editing

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First published: 13 September 2022
Citations: 2

Abstract

The study was designed to develop a pea protein- (PP) and chitosan (CS)-based bioactive composite film using purified Aronia melanocarpa polyphenols (AMP) as a bioactive ingredient (PP-CS-AMP). First, AMP was extracted and purified, and the antioxidant properties of AMP were tested, and the result indicated that AMP showed stronger antioxidant properties than vitamin C (Vc). Second, five kinds of PP-CS film were developed: PP including unextruded pea protein (NEP), extrusion pea protein (EP), papain co-extruded pea protein (PEP), neutral protease co-extruded pea protein (NPEP), and double enzyme combined extrusion pea protein (DCEP). The five kinds of PP-CS film were characterized and evaluated for various functions and properties; principal component analysis and membership evaluation were used to analyze the comprehensive scores of the films. It was observed that the NPEP-CS film obtained the highest score. Extrusion and enzyme extrusion treatments improved the structure and properties of PP, and therefore enhanced the properties of PP-CS film. Finally, NPEP-CS-AMP film was developed (AMP content 0, 4.0% and 8.0%, w/v) and tested for their performance, and the results suggested that the addition of 4.0% AMP gives the NPEP-CS-AMP film excellent physical and antioxidant properties.

Novelty impact statement

  • Aronia melanocarpa polyphenols (AMP), as a bioactivity ingredient was firstly incorporated into pea protein (PP) - chitosan (CS) film.
  • Extrusion and enzyme extrusion can improve the structure and properties of PP, which is beneficial to improvement on the properties of PP-CS film.
  • NPEP-CS-AMP film exhibit excellent physical properties, structural performance and high antioxidant activity.

CONFLICT OF INTEREST

The authors have declared no conflicts of interest for this article.

DATA AVAILABILITY STATEMENT

Data sharing not applicable – no new data generated, or the article describes entirely theoretical research.

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