Selection of a suitable solvent for bioactive compounds extraction of myrtle (Myrtus communis L.) leaves using ultrasonic waves
Corresponding Author
Maliheh Yaghoobi
Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Zanjan, Iran
Correspondence
Maliheh Yaghoobi, Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Room M237, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Mohsen Sanikhani, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Room 21, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Writing - original draft, Writing - review & editing
Search for more papers by this authorCorresponding Author
Mohsen Sanikhani
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Correspondence
Maliheh Yaghoobi, Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Room M237, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Mohsen Sanikhani, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Room 21, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Contribution: Conceptualization, Data curation, Investigation, Methodology, Resources, Supervision, Validation, Writing - review & editing
Search for more papers by this authorZeinab Samimi
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Contribution: Investigation, Visualization
Search for more papers by this authorAzizollah Kheiry
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Contribution: Project administration
Search for more papers by this authorCorresponding Author
Maliheh Yaghoobi
Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Zanjan, Iran
Correspondence
Maliheh Yaghoobi, Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Room M237, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Mohsen Sanikhani, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Room 21, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Writing - original draft, Writing - review & editing
Search for more papers by this authorCorresponding Author
Mohsen Sanikhani
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Correspondence
Maliheh Yaghoobi, Department of Chemical Engineering, Faculty of Engineering, University of Zanjan, Room M237, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Mohsen Sanikhani, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Room 21, University Blvd, P.O. Box 45371-38791, Zanjan, Iran.
Email: [email protected]
Contribution: Conceptualization, Data curation, Investigation, Methodology, Resources, Supervision, Validation, Writing - review & editing
Search for more papers by this authorZeinab Samimi
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Contribution: Investigation, Visualization
Search for more papers by this authorAzizollah Kheiry
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Contribution: Project administration
Search for more papers by this authorAbstract
The aim of this study was to evaluate the effect of solvent properties on bioactive extraction of myrtle leaves using ultrasound waves. Experimental design was performed using response surface methodology and optimized by Box-Behnken design. The effect of extraction variables such as temperature (30, 45, and 60°C), time (5, 20, and 35 min) and solid to solvent ratio (0.2, 0.3, and 0.4 g/10 ml) was examined for estimation of total phenolic content (TPC) and total flavonoid content (TFC) of water, ethanol, methanol, and ethyl acetate as extraction solvents using ultrasonic waves. The result of water extract showed highest contents of TPC (269.36 ± 3.83 mg gallic acid equivalent/g dry weight) and TFC (21.39 ± 0.43 mg quercetin equivalent/g dry weight) compared with other solvents. The optimal condition was obtained at the extraction temperature of 58°C, extraction time of 34 min, and solid to solvent ratio of 0.2 g/10 ml.
Novelty impact statement
- Water as a cheap and green solvent resulted in high total phenolic and flavonoid compounds in myrtle extract.
- Ultrasonic waves facilitated penetration of water into leaf tissue.
- The solvent polarity was most important in extraction efficiency of phenolic and flavonoid compounds.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
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