A novel L-asparaginase from the symbiotic Enterobacter aerogenes isolated from Eucheuma sp.
Corresponding Author
Mashuri Masri
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Correspondence
Mashuri Masri, Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, South Sulawesi, 92113, Indonesia.
Email: [email protected]
Contribution: Methodology, Resources, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorFatmawati Nur
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Search for more papers by this authorJoko Widodo
Laboratory of Technology Department, STIKES Mega Rezky, Makassar, Indonesia
Contribution: Funding acquisition, Methodology, Validation, Writing - original draft
Search for more papers by this authorEkafadly Jusuf
School of Management and Business (STIE) Amkop, Makassar, Indonesia
Search for more papers by this authorWindy Sahar
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Search for more papers by this authorNurul Wahida
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Data curation, Resources, Writing - original draft
Search for more papers by this authorRisnawati Risnawati
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Search for more papers by this authorSiti Nurbaya
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Data curation, Resources
Search for more papers by this authorTuti Asriani Asri
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Conceptualization, Resources
Search for more papers by this authorNurul Fadly
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Conceptualization, Project administration
Search for more papers by this authorCorresponding Author
Mashuri Masri
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Correspondence
Mashuri Masri, Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, South Sulawesi, 92113, Indonesia.
Email: [email protected]
Contribution: Methodology, Resources, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorFatmawati Nur
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Search for more papers by this authorJoko Widodo
Laboratory of Technology Department, STIKES Mega Rezky, Makassar, Indonesia
Contribution: Funding acquisition, Methodology, Validation, Writing - original draft
Search for more papers by this authorEkafadly Jusuf
School of Management and Business (STIE) Amkop, Makassar, Indonesia
Search for more papers by this authorWindy Sahar
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Search for more papers by this authorNurul Wahida
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Data curation, Resources, Writing - original draft
Search for more papers by this authorRisnawati Risnawati
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Search for more papers by this authorSiti Nurbaya
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Data curation, Resources
Search for more papers by this authorTuti Asriani Asri
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Conceptualization, Resources
Search for more papers by this authorNurul Fadly
Department of Biology, Faculty of Science and Technology, Alauddin State Islamic University, Makassar, Indonesia
Contribution: Conceptualization, Project administration
Search for more papers by this authorAbstract
This study was aimed to purify and characterize the enzyme L-asparaginase from endophytic microorganisms of Eucheuma sp. in Puntondo Island, South Sulawesi, Indonesia. Research stages were ammonium sulfate precipitation, dialysis, and Sephadex G-75 gel filtration chromatography and Sephadex CMC-50 ion-exchange chromatography matrices. The result of test activities of ammonium sulfate fraction showed the highest range of 40%–60% the activity value of 6.71 IU/ml. The highest protein content was obtained on a 0%–20% fraction of 2.76 mg/ml. Temperature and pH assessments indicated that L-asparaginase had optimum activity at 37°C and pH 8, respectively. At the stage of dialysis, the highest protein content was 5.17 IU/ml in a 0%–20% fraction, and the highest enzyme activity was 1.23 IU/ml in a 20%–40% fraction. The results of gel filtration chromatography fractions G-75 were F5–F10, with a specific activity of 0.60 U/mg, 2.05 times higher purity level than the crude extract enzyme. The results of CMC-50 ion-exchange chromatography showed that F54 and F55 fractions had a specific activity of 106.97 and 295.38 U/mg, respectively, with a purity level of 366.34 and 1,011.57 times higher than the crude extract enzyme.
Novelty impact statement
Enterobacter aerogenes strain P5, an endophytic bacterium from Eucheuma sp., can produce pure L-asparaginase enzyme with a high specific activity applied in pharmaceuticals nutraceutical applications.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available in the supplementary material of this article.
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