ORIGINAL ARTICLE
Probiotic coffee ice cream as an innovative functional dairy food
Ahmed B. Shazly,
Mohamed T. Fouad,
Mostafa Elaaser,
Rehab S. Sayed,
Mahmoud Abd El-Aziz,
Corresponding Author
Ahmed B. Shazly
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Correspondence
Ahmed B. Shazly, Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, 33 El Bohouth St., Dokki, P.O. 12622, Giza, Egypt
.
Email: [email protected]
Search for more papers by this authorMohamed T. Fouad
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Search for more papers by this authorMostafa Elaaser
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Search for more papers by this authorRehab S. Sayed
Regional Centre for Food and Feed, Agriculture Research Centre, Giza, Egypt
Search for more papers by this authorMahmoud Abd El-Aziz
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Search for more papers by this authorAhmed B. Shazly,
Mohamed T. Fouad,
Mostafa Elaaser,
Rehab S. Sayed,
Mahmoud Abd El-Aziz,
Corresponding Author
Ahmed B. Shazly
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Correspondence
Ahmed B. Shazly, Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, 33 El Bohouth St., Dokki, P.O. 12622, Giza, Egypt
.
Email: [email protected]
Search for more papers by this authorMohamed T. Fouad
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Search for more papers by this authorMostafa Elaaser
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Search for more papers by this authorRehab S. Sayed
Regional Centre for Food and Feed, Agriculture Research Centre, Giza, Egypt
Search for more papers by this authorMahmoud Abd El-Aziz
Dairy Department, Food Industries & Nutrition Research Institute, National Research Centre, Giza, Egypt
Search for more papers by this authorAbstract
The use of concentrated coffee extract (CCE) with probiotic bacteria in the production of ice cream was evaluated as an innovative functional dairy food. The most prevalent phenolic compounds and B-complex vitamins in CCE were chlorogenic acid and folic acid, with 7.39 and 4.67 mg/ml, respectively. CCF showed strong antibacterial activity against both gram-positive and gram-negative bacteria as well as fungi. Ice cream formulated with 3% CCE showed higher overrun (41.76%), fat destabilization (15.14%), and melting rate (1.19 g/min). The addition of probiotic bacteria Bifidobacterium breve Bb-12 and Lactobacillus plantarum increased the mixture's viscosity, overrun and melting rate. CCE-containing ice creams exhibited significant antioxidant activity against the DPPH and ABTS radicals, with the increase being proportional to the CCE content. In comparison to ice cream containing 5% CCE, ice cream containing 3% CCE, with and without probiotic bacteria, had a favorable brown color, a smoother texture, less bitterness, and the desired coffee flavor. It can be concluded that 3% CCE with probiotic bacteria can be used to produce a functional ice cream with the desired coffee flavor and rich in natural bioactive compounds such as phenols and vitamins.
Novelty impact statement
- Probiotic coffee ice cream is a functional dairy food that contains necessary nutrients and is rich in phenolic compounds that display strong antioxidant and antimicrobial activity in addition to being supported by probiotic bacteria.
- Probiotic coffee ice cream is a distinct type of ice cream with the desired coffee flavor for most consumers and it improves the mental state and helps burn calories.
- In addition to its health benefits, the use of probiotic bacteria can improve the mixture's viscosity and increase the overrun of the resulting ice cream.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
Open Research
DATA AVAILABILITY STATEMENT
The datasets generated or analyzed during the current study are available in this manuscript.
REFERENCES
- Abd El-Aziz, M., Mahran, G. A., Asker, A. A., Sayed, A. F., & El-Hadad, S. S. (2013). Blending of butter oil with refined palm oil: Impact on physicochemical properties and oxidative stability. International Journal of Dairy Science, 8, 36–47.
- Abid, Y., Casillo, A., Gharsallah, H., Joulak, I., Lanzetta, R., Michela, M., Attia, H., & Azabou, S. (2018). Production and structural characterization of exopolysaccharides from newly isolated probiotic lactic acid bacteria. International Journal of Biological Macromolecules, 108, 719–728.
- Adapa, S., Dingeldein, H., Schmidt, K. A., & Herald, T. J. (2000). Rheological properties of ice cream mixes and frozen ice creams containing fat and fat replacers. Journal of Dairy Science, 83, 2224–2229.
- Akın, M. S. (2005). Effects of inulin and different sugar levels on viability of probiotic bacteria and the physical and sensory characteristics of probiotic fermented ice-cream. Milchwissenschaft, 60, 297–301.
- Ally Open. (2022). https://allyopen.com/blogs/get-inspired/common-chemical-reactions-in-coffee-roasting
- Angelin, J., & Kavitha, M. (2020). Exopolysaccharides from probiotic bacteria and their health potential. International Journal of Biological Macromolecule, 162, 853–865.
- Arbuckle, W. S. (1986). Ice cream ( 4th ed.). AVI Pub. Co., Inc.
- Attokaran, M. (2017). Coffee arabica L; C. robusta Lindeu (Rubiaceae) chapter 41, natural food flavors and colorants, book ( 2nd ed., pp. 152–155). John Wiley & Sons Ltd.
- Bajad, D. N., Kalyankar, S. D., Dehmukh, S. D., Bachanti, P. R., & Bajad, G. S. (2016). Impact of physico-chemical properties of mix on the final quality of ice-cream. Asian Journal of Dairy and Food Research, 35, 293–297.
10.18805/ajdfr.v35i4.6627 Google Scholar
- Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28, 25–30.
- Cakmakci, S., Topdas, E. F., Kalin, P., Han, H., Sekerci, P., Kose, L. P., & Gulcin, I. (2015). Antioxidant capacity and functionality of oleaster (Elaeagnus angustifolia L.) flour and crust in a new kind of fruity ice cream. International Journal of Food Science & Technology, 50, 472–481.
- Çam, M., İçyer, N. C., & Erdoğan, F. (2014). Pomegranate peel phenolics: Microencapsulation, storage stability and potential ingredient for functional food development. LWT - Food Science and Technology, 55, 117–123.
- Colak, N., Torun, H., Gruz, J., Strnad, M., Subrtova, M., Inceer, H., & Ayaz, F. A. (2016). Comparison of phenolics and phenolic acid profiles in conjunction with oxygen radical absorbing capacity (ORAC) in berries of Vaccinium arctostaphylos L. and V. myrtillus L. Polish Journal of Food and Nutrition Sciences, 66, 85–91.
- Danesh, E., Goudarzi, M., & Jooyandeh, H. (2017). Short communication: Effect of whey protein addition and transglutaminase treatment on the physical and sensory properties of reduced-fat ice cream. Journal of Dairy Science, 100, 5206–5211.
- Duangjaia, A., Suphromb, N., Wungrathc, J., Ontawonga, A., Nuengchamnongd, N., & Yosboonruange, A. (2016). Comparison of antioxidant, antimicrobial activities and chemical profiles of three coffee (Coffea arabica L.) pulp aqueous extracts. Integrative Medicine Research, 5, 324–331.
- Dudonne, S., Vitrac, X., Coutiere, P., Woillez, M., & Merillon, J. M. (2009). Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. Journal of Agricultural and Food Chemistry, 57, 1768–1774.
- El-Hadad, S. S., Tikhomirova, N. A., Tvorogova, A. A., Shobanova, T. V., & Abd, E.-A. M. (2020). Physical properties and microstructure of ice cream supplemented with minor components of wheat germ oil. International Journal of Dairy Science, 15, 189–199.
- El-Shenawy, M., Abd El-Aziz, M., Elkholy, W., & Fouad, M. T. (2016). Probiotic ice cream made with tiger-nut (Cyperus esculentus) extract. American Journal of Food Technology, 11, 204–212.
- Erskine, E., Subaşı, B. G., Vahapoglu, B., & Capanoglu, E. (2022). Coffee phenolics and their interaction with other food phenolics: Antagonistic and synergistic effects. ACS Omega, 7, 1595–1601.
- Fiol, C., Prado, D., Romero, C., Laburu, N., Mora, M., & Alava, J. I. (2017). Introduction of a new family of ice creams. International Journal of Gastronomy and Food Science, 7, 5–10.
- Floegel, A., Kim, D., Chung, S., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal Food Composition and Analysis, 24, 1043–1048.
- Geremu, M., Tola, Y. B., & Sualeh, A. (2016). Extraction and determination of total polyphenols and antioxidant capacity of red coffee (Coffea arabica L.) pulp of wet processing plants. Chemical and Biological Technologies in Agriculture, 3, 25.
10.1186/s40538-016-0077-1 Google Scholar
- Ghazya, O. A., Fouad, M. T., Saleha, H. H., Kholif, A. E., & Morsy, T. A. (2021). Ultrasound-assisted preparation of anise extract nanoemulsion and its bioactivity against different pathogenic bacteria. Food Chemistry, 341, 128259–128266.
- Gmoser, R., Bordes, R., Nilsson, G., Altsk, A., Stading, M., Lorén, N., & Berta, M. (2016). Effect of dispersed particles on instant coffee foam stability and rheological properties. European Food Research and Technology, 243, 115–121.
- Golestani, M., & Pourahmad, R. (2016). Comparison of three treatments (two fermented treatments and one nonfermented treatment) in production of synbiotic ice cream. Journal of Food Processing and Preservation, 41, e12839.
- Güven, M., Kalender, M., & Taşpinar, T. (2018). Effect of using different kinds and ratios of vegetable oils on ice cream quality characteristics. Foods, 7, 104.
- Hagen, M., & Narvhus, J. A. (1999). Production of ice cream containing probiotic bacteria. Milchwissenschaft, 54, 265–268.
- Hashim, I. B., & Al Shamsi, K. D. (2016). Physiochemical and sensory properties of ice-cream sweetened with date syrup. Journal of Food Processing & Technology, 2, 1–4.
- Hofmann, T., Frank, O., Blumberg, S., Kunert, C., & Zehentbauer, G. (2007). Molecular insights into the chemistry producing harsh bitter taste compounds of strongly roasted coffee. In Proceedings of the 8th Wartburg symposium on flavor chemistry and biology, Eisenach, Germany, February 27–March 2, 2007. (pp. 154–159). Dt. Forschungsanstalt fur Lebensmittelchemie.
- ISO 21528-2. (2017). Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of Enterobacteriaceae – Colony-count method. ISO 21528-2:2017. https://www.iso.org/standard/63504.html
- ISO 4832. (2006). Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of β-glucuronidase positive Escherichia coli – Part 2: Colony-count technique at 44°C using 5-bromo-4-chloro-3-indolyl Β-Dglucuronide. ISO 4832:2006. https://www.iso.org/standard/38282.html
- ISO 6579-1. (2017). Microbiology of food and animal feeding stuffs – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp. ISO 6579-1:2017. https://www.iso.org/standard/56712.html
- ISO 6888-3. (2003). Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) – Part 1: Technique using Baird-Parker agar medium. ISO 6888-3:2003. https://www.iso.org/standard/33174.html
- Khedkar, C. D., Kalyankar, S. D., & Deosarkar, S. S. (2016). Buffalo milk. In B. Caballero, P. Finglas, & F. Toldrá (Eds.), Encyclopedia of food and health (Vol. 1, pp. 522–528). Elsevier Ltd.
10.1016/B978-0-12-384947-2.00093-3 Google Scholar
- Khider, M., Ahmed, N., & Metry, W. A. (2021). Functional ice cream with coffee-related flavor. Food and Nutrition Sciences, 12, 826–847.
- Komes, D., & Bušić, A. (2014). Antioxidants in coffee. In: Processing and Impact on Antioxidants in Beverages (pp. 25–32). Oxfored, Academic Press, Elsevier, San Diego.
10.1016/B978-0-12-404738-9.00003-9 Google Scholar
- Lou, Z., Wang, H., Zhu, S., Ma, C., & Wang, Z. (2011). Antibacterial activityand mechanism of action of chlorogenic acid. Journal of Food Science, 76, 398–403.
- Muhardina, V., Sari, P. M., Aisyah, Y., & Haryani, S. (2019). Physical characteristic of probiotic ice cream substituted by encapsulated lactic acid bacteria (LAB) with variety of aging time. Journal of Physics: Conference Series, 1232, 012042.
- Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C., & Yolken, H. R. (1995). Manual of clinical microbiology ( 6th ed., pp. 15–18). ASM Press.
- Muse, M. R., & Hartel, R. W. (2004). Ice cream structural elements that affect melting rate and hardness. Journal of Dairy Science, 87, 1–10.
- Naeem, M. A., Hassan, L. K., & Abd El-Aziz, M. (2019). Enhancing the pro-health and physical properties of ice cream fortified with concentrated golden berry juice. Acta Scientiarum Polonorum, Technologia Alimentaria, 18, 97–107.
- Nogueira, M., & Trugo, L. C. (2003). Distribuição de isômeros de ácido clorogênico e teores de cafeína e trigonelina em cafés solúveis Brasileiros. Sociedade Brasileira de Ciência e Tecnologia de Alimentos, Campinas, 23, 296–299.
- Perfect Daily Grind. (2017). https://perfectdailygrind.com/2017/11/coffee-science-breaking-down-where-flavor-comes-from/
- Re, R., Pellegrini, N., & Proteggente, A. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237.
- Reische, D. W., Lillard, D. A., & Eitenmiller, R. R. (2002). Antioxidants. In C. C. Akoh & D. B. Min (Eds.), Food lipids: Chemistry, nutrition and biotechnology (pp. 489–516). Marcel Dekker.
10.1201/9780203908815.ch15 Google Scholar
- Roginsky, V., & Lissi, E. A. (2005). Review of methods to determine chain-breaking antioxidant activity in food. Food Chemistry, 92, 235–254.
- Runti, G., Pacor, S., Colomban, S., Gennaro, R., Navarini, L., & Scocchi, M. (2015). Arabica coffee extract shows antibacterial activity against Staphylococcus epidermidis and Enterococcus faecalis and low toxicity towards a human cell line. LWT - Food Science and Technology, 62, 108–114.
- Sagdic, O., Ozturk, I., Cankurt, H., & Tornuk, F. (2012). Interaction between some phenolic compounds and probiotic bacterium in functional ice cream production. Food and Bioprocess Technology, 5, 2964–2971.
- Salem, M. M. E., Fathi, F. A., & Awad, R. A. (2005). Production of probiotic ice cream. Polish Journal of Food and Nutrition Sciences, 55(3), 267–271.
- SAS. (2008). The SAS system for windows, release 9.2. SAS Institute.
- Soni, R., Jain, N. K., Shah, V., Soni, J., & Suthar, D. (2020). Development of probiotic yogurt: Effect of strain combination on nutritional, rheological, organoleptic and probiotic properties. Journal of Food Science and Technology, 57, 2038–2050.
- Statista. (2021). https://www.statista.com/statistics/292595/global-coffee-consumption. Accessed on 14 Dec 2021.
- Sultana, B., & Anwar, F. (2008). Flavonols (kaempferol, quercetin, myricetin) contents of selected fruits, vegetables and medicinal plants. Food Chemestry, 108, 879–884.
- Sun-Waterhouse, D., Edmonds, L., Wadhwa, S. S., & Wibisono, R. (2013). Producing ice cream using a substantial amount of juice from kiwifruit with green, gold or red flesh. Food Research International, 50, 647–656.
- Talbot, G., Smith, K., & Bhaggan, K. (2002). Influence of minor components on fat crystallization. Lipid Technology., 24, 83–85.
10.1002/lite.201200180 Google Scholar
- Tipchuwong, N., Chatraporn, C., Ngamchuachit, P., & Tansawat, R. (2017). Increasing retention of vitamin D3 in vitamin D3 fortified ice cream with milk protein emulsifier. International Dairy Journal, 74, 74–79.
- Trandafir, I., Nour, V., & Ionica, M. E. (2013). Antioxidant capacity, phenolic acids and caffeine contents of some commercial coffees available on the Romanian market. Archivos Latinoamericanos de Nutrición, 63, 87–94.
- Ullah, R., Nadeem, M., & Imran, M. (2017). Omega-3 fatty acids and oxidative stability of ice cream supplemented with olein fraction of chia (Salvia hispanica L.) oil. Lipids in Health and Disease, 16, 34.
- Warren, M. M., & Hartel, R. W. (2018). Effects of emulsifier, overrun and dasher speed on ice cream microstructure and melting properties. Journal of Food Science, 83, 639–647.
- Wehr, H. M., & Frank, J. H. (2004). Standard methods for the examination of dairy products ( 17th ed.). APHA.
10.2105/9780875530024 Google Scholar
- Williamson, G., Dionisi, F., & Renouf, M. (2011). Flavanols from green tea and phenolic acids from coffee: Critical quantitative evaluation of the pharmacokinetic data in humans after consumption of single doses of beverages. Molecular Nutrition & Food Research, 55, 864–873.
- Wolfenden, E. (2019). How much vitamin B complex is safe to take in a day?. https://www.livestrong.com/article/483285-how-much-vitamin-b-complex-should-a-person-take-in-a-day/
- Wu, B., Freire, D. O., & Hartel, R. W. (2019). The effect of overrun, fat destabilization and ice cream mix viscosity on entire meltdown behavior. Journal Food Science, 84, 2562–2571.
- Yahayu, M., Hamid, Z. B. S., El Adawi, H., Dailin, D. J., Abd Malek, R., Hanapi, S. Z., Boumehira, A., Ngadiran, S., Yusoff, I. M., Ho, T., & El Enshasy, H. (2020). Antioxidant activity in green and roasted coffee: A critical review. Bioscience Research, 17, 2249–2263.
