Quantification of rice α-globulin allergen using liquid chromatography–tandem mass spectrometry combined with cysteine-specific modifier and extended stable isotope-labeled peptide
Huan Yang
Jiangxi Agricultural University, Nanchang, China
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Data curation, Investigation, Methodology, Validation
Search for more papers by this authorZhao-yun Cao
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Investigation, Methodology, Validation
Search for more papers by this authorRen-xiang Mou
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Investigation, Methodology, Validation
Search for more papers by this authorZhen-zhen Cao
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Conceptualization
Search for more papers by this authorCorresponding Author
Ming-xue Chen
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Correspondence
Ming-xue Chen, China National Rice Research Institute, No 359, Tiyuchang Road, Hangzhou, Zhejiang 310006, PR China.
Email: [email protected]
Contribution: Funding acquisition, Investigation, Methodology, Validation
Search for more papers by this authorHuan Yang
Jiangxi Agricultural University, Nanchang, China
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Data curation, Investigation, Methodology, Validation
Search for more papers by this authorZhao-yun Cao
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Investigation, Methodology, Validation
Search for more papers by this authorRen-xiang Mou
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Investigation, Methodology, Validation
Search for more papers by this authorZhen-zhen Cao
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Contribution: Conceptualization
Search for more papers by this authorCorresponding Author
Ming-xue Chen
Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou, China
Correspondence
Ming-xue Chen, China National Rice Research Institute, No 359, Tiyuchang Road, Hangzhou, Zhejiang 310006, PR China.
Email: [email protected]
Contribution: Funding acquisition, Investigation, Methodology, Validation
Search for more papers by this authorAbstract
An absolute quantification of α-globulin in rice matrices based on the liquid chromatography–tandem mass spectrometry (LC–MS/MS) utilizing extended stable isotope-labeled (SIL) peptides were studied. Two modified signature peptides, derived from the cysteine-specific modification by IAA during the tryptic digestion process, were identified and selected as the surrogates of α-globulin due to the 1.5–4.5-fold enhancement of intensity peak compared with its unmodified form. The digestion efficiency could be improved from 72.3–98.8% to 94.0–105.3%, and matrix interference normalized from −26.6–4.5% to −6.0–5.5% when the extended SIL peptides were used as ISs. The limit of detection (LOD) and limit of quantification (LOQ) for the α-globulin were 0.25 and 0.8 μg/g, respectively. The recoveries of α-globulin spiked at three levels were between 82 and 105%. The described method was successfully employed for the determination of α-globulin in rice and food product samples.
Novelty impact statement
Two modified peptides were selected as surrogates of α-globulin to absolutely quantify the α-globulin allergen in rice based on LC–MS/MS. The extended stable isotope-labeled peptide were used as internal standards to normalize variation from the tryptic digestion and matrix effects. The proposed approach showed excellent sensitivity and accuracy, and successfully applied to determination of the α-globulin in vary rice and food products.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
Open Research
DATA AVAILABILITY STATEMENT
The data that supports the findings of this study are available in the supplementary material of this article
Supporting Information
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| jfpp16362-sup-0001-Supinfo.docxWord 2007 document , 291.5 KB |
Appendix S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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