Effect of carboxymethyl cellulose/candelilla wax edible coating incorporated with ascorbic acid on the physicochemical and sensory qualities of prepackaged minimally processed carrots (Daucus carota L.) during cold storage
Corresponding Author
Dariusz Kowalczyk
Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland
Correspondence
Dariusz Kowalczyk, Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Skromna 8, Lublin 20-704, Poland.
Email: [email protected]
Search for more papers by this authorTomasz Skrzypek
Confocal and Electron Microscopy Laboratory, Centre for Interdisciplinary Research, John Paul II Catholic University of Lublin, Lublin, Poland
Search for more papers by this authorKatarzyna Łupina
Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland
Search for more papers by this authorCorresponding Author
Dariusz Kowalczyk
Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland
Correspondence
Dariusz Kowalczyk, Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Skromna 8, Lublin 20-704, Poland.
Email: [email protected]
Search for more papers by this authorTomasz Skrzypek
Confocal and Electron Microscopy Laboratory, Centre for Interdisciplinary Research, John Paul II Catholic University of Lublin, Lublin, Poland
Search for more papers by this authorKatarzyna Łupina
Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Lublin, Poland
Search for more papers by this authorAbstract
Minimally processed carrots (MPC) was coated with ascorbic acid-added carboxymethyl cellulose/candelilla wax emulsion, then placed in plastic boxes with perforated polyethylene wrap, and stored at 5°C. It was found that the horizontal cross-section of samples absorbed larger amounts of emulsion than the lateral surface. The individual wax globules of the coating deposited in the interior of the damaged (by peeling) cortical cells were visualized using scanning electron microscopy. The coating improved water vapor resistance of MPC, temporarily increased the respiratory rate, decreased the activity of polyphenol oxidase, and prevented the formation of “white blush.” The acidity, total phenolic content, and texture of the MPC were not affected by the coating treatment. In summary, the edible coating (along with packaging) can serve as a promising approach for maintaining the fresh-like characteristics of minimally processed carrots through 21 days of cold storage.
Practical applications
The treatment of minimally processed carrots with the carboxymethyl cellulose/candelilla wax/ascorbic acid emulsion results in increased moisture retention. Consequently, the coated carrots exhibit reduced weight loss, unchanged hardness, and no “white blush.” As expected, the coating inhibited the activity of polyphenol oxidase, most likely due to the MPC surface acidification. In summary, the edible coating (along with packaging) can serve as a promising approach for maintaining the fresh-like characteristics of minimally processed carrots through 21 days of cold storage. However, the noticeable disadvantage of the coated carrots is the waxy circular deposit around the pericycle and endodermis cells.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
REFERENCES
- Ali, H. M., El-Gizawy, A. M., El-Bassiouny, R. E. I., & Mahmoud, S. (2016). Inhibition mechanisms of the browning process in vegetables and fruits by various inhibitors. Journal of Nutrition & Food Sciences, 6(7), 9600.
- Avena-Bustillos, R. J., Cisneros-Zevallos, L. A., Krochta, J. M., & Saltveit, M. E. (1994). Application of casein-lipid edible film emulsions to reduce white blush on minimally processed carrots. Postharvest Biology and Technology, 4(4), 319–329. https://doi.org/10.1016/0925-5214(94)90043-4
- Chauhan, O. P., Raju, P. S., Ravi, N., Singh, A., & Bawa, A. S. (2011). Effectiveness of ozone in combination with controlled atmosphere on quality characteristics including lignification of carrot sticks. Journal of Food Engineering, 102, 43–48. https://doi.org/10.1016/j.jfoodeng.2010.07.033
- Cisneros-Zevallos, L., Saltveit, M. E., & Krochta, J. M. (1995). Mechanism of surface white discoloration of peeled (minimally processed) carrots during storage. Journal of Food Science, 60(2), 320–323. https://doi.org/10.1111/j.1365-2621.1995.tb05664.x
- Davis, E. A., & Gordon, J. (1977). Morphological comparison of two varieties of carrots during growth and storage: Scanning electron microscopy. Home Economics Research Journal, 6, 15–23. https://doi.org/10.1177/1077727X7700600102
- Devlieghere, F., Vermeulen, A., & Debevere, J. (2004). Chitosan: Antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiology, 21, 703–714. https://doi.org/10.1016/j.fm.2004.02.008
- Dhall, R. K. (2013). Advances in edible coatings for fresh fruits and vegetables: A review. Critical Reviews in Food Science and Nutrition, 53(5), 435–450. https://doi.org/10.1080/10408398.2010.541568
- Donhove, G., & Fennema, O. (1993). The effects of plasticizers on crystallinity, permeability, and mechanical properties of methylcellulose films. Journal of Food Processing and Preservation, 17, 247–257. https://doi.org/10.1111/j.1745-4549.1993.tb00729.x
- Fai, A. E. C., Alves de Souza, M. R., de Barros, S. T., Bruno, N. V., Ferreira, M. S. L., Gonçalves, É. C. B. A. (2016). Development and evaluation of biodegradable films and coatings obtained from fruit and vegetable residues applied to fresh-cut carrot (Daucus carota L.). Postharvest Biology and Technology, 112, 194–204. https://doi.org/10.1016/j.postharvbio.2015.09.021
- Fonseca, S. C., Oliveira, F. A. R., & Brecht, J. K. (2002). Modelling respiration rate of fresh fruits and vegetables for modified atmosphere packages: A review. Journal of Food Engineering, 52(2), 99–119. https://doi.org/10.1016/S0260-8774(01)00106-6
- Garcia, E., & Barrett, D. M. (2002). Preservative treatments for fresh-cut fruits and vegetables. In O. Lamikanra (Ed.), Fresh-cut fruits and vegetables: science, technology, and market (pp. 274–310). Boca Raton, FL: CRC Press. https://doi.org/10.1201/9781420031874.ch9
- Gawlik-Dziki, U., Złotek, U., & Świeca, M. (2008). Characterization of polyphenol oxidase from butter lettuce (Lactuca sativa var. capitata L.). Food Chemistry, 107(1), 129–135 https://doi.org/10.1016/j.foodchem.2007.07.068
- Ghaouth, A., Arul, J., Ponnampalam, R., & Boulet, M. (1991). Chitosan coating effect on storability and quality of fresh strawberries. Journal of Food Science, 56, 1618–1620. https://doi.org/10.1111/j.1365-2621.1991.tb08655.x
- Gómez-López, V. M., Devlieghere, F., Ragaert, P., & Debevere, J. (2007). Shelf-life extension of minimally processed carrots by gaseous chlorine dioxide. International Journal of Food Microbiology, 116(2), 221–227. https://doi.org/10.1016/j.ijfoodmicro.2006.12.008
- Howard, L. R., & Griffin, L. E. (1993). Lignin formation and surface discoloration of minimally processed carrot sticks. Journal of Food Science, 58(5), 1065–1067. https://doi.org/10.1111/j.1365-2621.1993.tb06113.x
- ISO 1842:1991. (1991). Fruit and vegetable products—Determination of pH.
- ISO 750:1998. (1998). Fruit and vegetable products—Determination of titratable acidity.
- Judd, D. B., & Wyszecki, G. (1963). Color in business, science, and industry. New York, NY: Wiley-Interscience.
10.1063/1.3050671 Google Scholar
- Kanayama, Y., & Kochetov, A. (2015). Abiotic stress biology in horticultural plants. Tokyo, Japan: Springer.
10.1007/978-4-431-55251-2 Google Scholar
- Kasim, R., Kasim, M. U., & Uyar, G. E. Ö. (2015). Postharvest ascorbic acid treatments on color and sugar changes on fresh-cut carrot. International Journal of Research in Agriculture and Food Sciences, 2(20), 1–8.
- Klaiber, R., Baur, S., Magel, L., Hammes, W., & Carle, R. (2004). Quality of shredded, packaged carrots as affected by different washing treatments. Journal of Food Science, 69, 161–166. https://doi.org/10.1111/j.1365-2621.2004.tb06358.x
- Kowalczyk, D. (2016). Biopolymer/candelilla wax emulsion films as carriers of ascorbic acid—A comparative study. Food Hydrocolloids, 52, 543–553. https://doi.org/10.1016/j.foodhyd.2015.07.034
- Kowalczyk, D., & Baraniak, B. (2014). Effect of candelilla wax on functional properties of biopolymer emulsion films—A comparative study. Food Hydrocolloids, 41, 195–209. https://doi.org/10.1016/j.foodhyd.2014.04.004
- Kowalczyk, D., Kordowska-Wiater, M., Kałwa, K., Skrzypek, T., Sikora, M., & Łupina, K. (2019). Physiological, qualitative, and microbiological changes of minimally processed Brussels sprouts in response to coating with carboxymethyl cellulose/candelilla wax emulsion. Journal of Food Processing and Preservation, 43(8), https://doi.org/10.1111/jfpp.14004
- Kowalczyk, D., Kordowska-Wiater, M., Zięba, E., & Baraniak, B. (2017). Effect of carboxymethylcellulose/candelilla wax coating containing potassium sorbate on microbiological and physicochemical attributes of pears. Scientia Horticulturae, 218, 326–333. https://doi.org/10.1016/j.scienta.2017.02.040
- Kowalczyk, D., Kordowska-Wiater, M., Złotek, U., & Skrzypek, T. (2018). Antifungal resistance and physicochemical attributes of apricots coated with potassium sorbate-added carboxymethyl cellulose-based emulsion. International Journal of Food Science and Technology, 53(3), 728–734. https://doi.org/10.1111/ijfs.13648
- Kowalczyk, D., Świeca, M., Cichocka, J., & Gawlik-Dziki, U. (2013). The phenolic content and antioxidant activity of the aqueous and hydroalcoholic extracts of hops and their pellets. Journal of the Institute of Brewing, 119(3), 103–110. https://doi.org/10.1002/jib.73
- Landi, M., Degl'innocenti, E., Guglielminetti, L., & Guidi, L. (2013). Role of ascorbic acid in the inhibition of polyphenol oxidase and the prevention of browning in different browning-sensitive Lactuca sativa var. capitata (L.) and Eruca sativa (Mill.) stored as fresh-cut produce. Journal of the Science of Food and Agriculture, 93, 1814–1819. https://doi.org/10.1002/jsfa.5969
- Larsen, H., & Wold, A. (2015, December). Optimisation of packaging for carrot roots (Daucus carota L.) stored at different temperatures. Conference: 27th IAPRI Symposium on Packaging, Valencia, Spain.
- Leceta, I., Molinaro, S., Guerrero, P., Kerry, J. P., & De la Caba, K. (2015). Quality attributes of map packaged ready-to-eat baby carrots by using chitosan-based coatings. Postharvest Biology and Technology, 100, 142–150. https://doi.org/10.1016/j.postharvbio.2014.09.022
- Li, P., & Barth, M. M. (1998). Impact of edible coatings on nutritional and physiological changes in lightly-processed carrots. Postharvest Biology and Technology, 14(1), 51–60. https://doi.org/10.1016/S0925-5214(98)00020-9
- Pilon, L., Oetterer, M., Gallo, C. R., & Spoto, M. H. F. (2006). Shelf life of minimally processed carrot and green pepper. Ciencia e Tecnologia de Alimentos, 26(1), 150–158. https://doi.org/10.1590/S0101-20612006000100025
- Queiroz, C., Mendes Lopes, M. L., Fialho, E., & Valente-Mesquita, V. L. (2008). Polyphenol oxidase: Characteristics and mechanisms of browning control. Food Reviews International, 24(4), 361–375. https://doi.org/10.1080/87559120802089332
- Reyes, A., Vega, R., Bustos, R., & Araneda, C. (2008). Effect of processing conditions on drying kinetics and particle microstructure of carrot. Drying Technology, 26(10), 1272–1285. https://doi.org/10.1080/07373930802307282
- Schlimme, D. V., & Rooney, M. L. (1994). Packaging of minimally processed fruits and vegetables. In F. Yildiz & R. C. Wiley (Eds.), Minimally processed refrigerated fruits & vegetables (pp. 135–182). Boston, MA: Springer.
10.1007/978-1-4615-2393-2_4 Google Scholar
- Schouten, S. P. (1985). Significance of ethylene in post-harvest handling of vegetables. In J. A. Roberts & G. A. Tucker (Eds.), Ethylene and plant development (pp. 353–362). Oxford, UK: Butterworth-Heinemann.
10.1016/B978-0-407-00920-2.50033-0 Google Scholar
- Shigematsu, E., Dorta, C., Rodrigues, F. J., Cedran, M. F., Giannoni, J. A., Oshiiwa, M., & Mauro, M. A. (2018). Edible coating with probiotic as a quality factor for minimally processed carrots. Journal of Food Science and Technology, 55(9), 3712–3720. https://doi.org/10.1007/s13197-018-3301-0
- Simões, A. D. N., Tudela, J. A., Allende, A., Puschmann, R., & Gil, M. I. (2009). Edible coatings containing chitosan and moderate modified atmospheres maintain quality and enhance phytochemicals of carrot sticks. Postharvest Biology and Technology, 51(3), 364–370. https://doi.org/10.1016/j.postharvbio.2008.08.012
- Simões, A., Ventrella, M., Moretti, C., Carnelossi, M., & Puschmann, R. (2010). Anatomical and physiological evidence of white blush on baby carrot surfaces. Postharvest Biology and Technology, 55, 45–52. https://doi.org/10.1016/j.postharvbio.2009.07.007
- Surjadinata, B. B., & Cisneros-Zevallos, L. (2012). Biosynthesis of phenolic antioxidants in carrot tissue increases with wounding intensity. Food Chemistry, 134(2), 615–624. https://doi.org/10.1016/j.foodchem.2012.01.097
- Villafañe, F. (2017). Edible Coatings for Carrots. Food Reviews International, 33, 84–103. https://doi.org/10.1080/87559129.2016.1150291
- Wu, X. J., Sun, S., Xing, G. M., Wang, G. L., Wang, F., Xu, Z. S., … Xiong, A. S. (2017). Elevated carbon dioxide altered morphological and anatomical characteristics, ascorbic acid accumulation, and related gene expression during taproot development in carrots. Frontiers in Plant Science, 7, 1–11. https://doi.org/10.3389/fpls.2016.02026
- Yan, H., Zhang, W., Kan, X., Dong, L., Jiang, Z., Li, H., … Cheng, R. (2011). Sorption of methylene blue by carboxymethyl cellulose and reuse process in a secondary sorption. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 380, 143–151. https://doi.org/10.1016/j.colsurfa.2011.02.045
- Zhang, Q., Tan, S., McKay, A., & Yan, G. (2005). Carrot browning on simulated market shelf and during cold storage. Journal of the Science of Food and Agriculture, 85(1), 16–20. https://doi.org/10.1002/jsfa.1931
