Evaluation of the quality of fresh and frozen wheatgrass juices depending on the time of grass harvest
Corresponding Author
Łukasz Skoczylas
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Correspondence Łukasz Skoczylas, Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow 30-149, Poland. Email: [email protected]Search for more papers by this authorAnna Korus
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorMałgorzata Tabaszewska
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorKatarzyna Gędoś
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorEdyta Szczepańska
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorCorresponding Author
Łukasz Skoczylas
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Correspondence Łukasz Skoczylas, Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow 30-149, Poland. Email: [email protected]Search for more papers by this authorAnna Korus
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorMałgorzata Tabaszewska
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorKatarzyna Gędoś
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorEdyta Szczepańska
Department of Fruit, Vegetable and Mushroom Processing, University of Agriculture in Krakow, 122 Balicka Street, Krakow, 30-149, Poland
Search for more papers by this authorFunding information: Ministry of Science and Higher Education of the Republic of Poland
Abstract
Fresh and frozen wheatgrass juice (WgJ) stored for three months were examined in terms of: polyphenolic components, total chlorophylls and carotenoids, antioxidant and enzymatic activity and color. Juices were prepared from summer and winter grass, at two growth stages (16 and 21 cm). Raw WgJ (100 g) contained: 5.04 dry matter; 4.59 total extract; and 0.820 ash. Raw WgJ (1 L) contained also 391.4 mg chlorophylls; 79.1 mg carotenoids; 920.9 mg total polyphenols, with a mean antioxidant activity of 9.57 and 93.07 µM Troloxeq/mL (DPPH) and (ABTS) respectively; catalase and peroxidase activity were 114.94 and 0.13 U, respectively. Summer WgJ (fresh and frozen) had higher chlorophylls, carotenoids, total polyphenols concentration, increased enzymatic activity, better color than winter WgJ, but lower levels of dry matter, ash, and extract. Freezing and storage of juices significantly reduced the level of dry matter, chlorophylls, carotenoids, antioxidant, and catalase activity. Color deterioration was also noted.
Practical applications
Sprouts and wheat grass are young plants, including active enzymes, easily absorbed vitamins, minerals and phytochemicals. Due to the lack of human enzymes digesting cellulose, which large amounts are found in the grass a better alternative is to drink wheatgrass juice. They represent one of the latest discoveries in nutrition. The best way is to drink fresh juice immediately after production, so they do not lose during storage labile compounds. The consumption of wheatgrass juice brings a lot of benefits. Not everyone can afford a grass breeding and production of juice at home. A long list of beneficial properties of juice obtained from wheat grass making it the product of an increasingly desired by consumers. The process of freezing is still one of the least invasive and universal methods for maintaining the product almost unchanged while significantly extend the life of.
REFERENCES
- Abbasi, N. A., Tareen, M. J., & Hafiz, I. A. (2012). Postharvest application of salicylic acid enhanced antioxidant enzyme activity and maintained quality of peach cv. ‘Flordaking’ fruit during storage. Scientia Horticulturae, 142, 221–228.
- Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121–128.
-
Akcan Kardas, T., &
Durucasu, I. (2014). A new analytical method for the determination of phenolic compounds and their antioxidant activities in different wheat grass varieties. Ekoloji, 23(90), 73–80.
10.5053/ekoloji.2014.909 Google Scholar
- Association of Official Analytical Chemistry (AOAC). (1995). Official methods of analysis ( 16th ed.). Arlington, USA: Author.
- Ardestani, A., & Yazdanparast, R. (2007). Antioxidant and free radical scavenging potential of Achillea santolina extracts. Food Chemistry, 104, 21–29.
- Bar-Sela, G., Tsalic, M., Fried, G., & Goldberg, H. (2007). Wheat grass juice may improve hematological toxicity related to chemotherapy in breast cancer patients: A pilot study. Nutrition and Cancer, 58(1), 43–48.
- Brewer, M. S., Begum, S., & Bozeman, A. (1995). Microwave and conventional blanching effects on chemical, sensory, and color characteristics of frozen broccoli. Journal of Food Quality, 18, 479–493.
- Choi, K. S., & Lee, H. Y. (1999). Characteristics of useful components in the leaves of baechohyang (Agastache rugosa, O. Kuntze). Journal of the Korean Society of Food Science and Nutrition, 28, 326–332.
- Das, A., Raychaudhuri, U., & Chakraborty, R. (2012). Antimicrobial effect of edible plant extract on the growth of some foodborne bacteria including pathogens. Nutrafoods, 12, 83–88.
- De Vogel, J., Jonker-Termon, D. S. M. L., van Lieshout, E. M. M., Katan, M. B., & van der Meer, R. (2005). Green vegetables, red meat and colon cancer: Chlorophyll prevents the cytotoxic and hyperproliferative effects of haem in rat colon. Carcinogenesis, 26(2), 387–393.
- Del Bãno, M. J., Lorente, J., Castillo, J., Benavente-Garcia, O., Rio, J. A- D., Ortuno, A., … Gerard, D. (2003). Phenolic diterpenes, flavones, and rosmarinic acid distribution during the development of leaves, flowers, stems, and roots of Rosmarinus officinalis. Antioxidant activity. Journal of Agricultural and Food Chemistry, 51, 4247–4253.
- Dutta, D., Chaudhuri, U. R., & Chakraborty, R. (2005). Structure, health benefits, antioxidant property and processing and storage of carotenoids. African Journal of Biotechnology, 4, 1510–1520.
- Goswami, T. K. (2010). Role of cryogenics in food processing and preservation. International Journal of Food Engineering, 6, 1–29.
- Haiying, W., Shaozhi, Z., & Guangming, C. (2007). Experimental study on freezing characteristics of four kinds of vegetables. LWT—Food Science and Technology, 40, 1112–1116.
- Hanninen, O., Rauma, A. L., Kaartinen, K., & Nenonen, M. (1999). Vegan diet in physiological health promotion. Acta Physiologica Hungarica, 86, 171–180.
- Iqbal, S., & Bhanger, M. I. (2006). Effect of season and production location on antioxidant activity of Moringa oleifa leaves grown in Pakistan. Journal of Food Composition and Analysis, 19, 544–551.
- Kidmose, U., Knuthsen, P., Edelenbos, M., Justesen, U., & Hegelund, E. (2001). Carotenoids and flavonoids in organically grown spinach (Spinacia oleracea L.) genotypes after deep frozen storage. Journal of the Science of Food and Agriculture, 81, 918–923.
- Klimczak, I., Małecka, M., Szlachta, M., & Gliszczyńska-Świgło, A. (2007). Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. Journal of Food Composition and Analysis, 20, 313–322.
- Kolniak, J. (2008). Wpływ sposobu zamrażania, rozmrażania oraz dodatków krioochronnych na zawartość polifenoli ogółem, antocyjanów i pojemnośćprzeciwutleniającą mrożonek truskawkowych. ŻYWNOŚĆ. Nauka. Technologia. Jakość, 5(60), 135–148.
- Kulkarni, S. D., Tilak, J. C., Acharya, R., Nilima, S., Rajurkar, N. S., Devasagayam, T. P. A., & Reddy, A. V. R. (2006). Evaluation of the antioxidant activity of wheatgrass (Triticum aestivum L.) as a function of growth under different conditions. Phytotherapy Research, 20, 218–227.
- Lichtenthaler, H. K., & Buschmann, C. (2001a). Extraction of photosynthetic tissues: Chlorophylls and carotenoids. Current Protocols in Food Analytical Chemistry, S1, F4.2.1–F4.2.6.
- Lichtenthaler, H. K., & Buschmann, C. (2001b). Chlorophylls and carotenoids: Measurement and characterization by UV–VIS spectroscopy. Current Protocols in Food Analytical Chemistry, S1, F4.3.1–F4.3.8.
- Marx, M., Schieber, A., & Carle, R. (2000). Quantitative determination of carotene stereoisomers in carrot juices and vitamin supplemented (ATBC) drinks. Food Chemistry, 70, 403–408.
- Meyerowitz, S. (2007). Wheatgrass natures finest medicine ( 7th ed.). USA: Sproutman Publications.
- Mukhopadhyay, S., Basak, J., Kar, M., Mandal, S., & Mukhopadhyay, A. (2009). The role of iron chelation activity of wheatgrass juice in patients with myelodysplastic syndrome. Journal of Clinical Oncology, 27(15S), 7012.
- Ng, S. C., Lam, Y. T., Tsoi, K. K. F., Chan, F. K. L., Sung, J. J. Y., & Wu, J. C. Y. (2013). Systematic review: The efficacy of herbal therapy in inflammatory bowel disease. Alimentary Pharmacology & Therapeutics, 38(8), 854–863.
- Padda, M. S., & Picha, D. H. (2008). Effect of low temperature storage on phenolic composition and antioxidant activity of sweetpotatoes. Postharvest Biology and Technology, 47, 176–180.
- Padalia, S., Drabu, S., Raheja, I., Gupta, A., & Dhamija, M. (2010). Multitude potential of wheatgrass juice (green blood): An overview. Chronicles of Young Scientists, 1(2), 23–28.
-
Pekkarinen, S. S.,
Heinonen, I. M., &
Hopia, A. I. (1999). Flavonoids quercetin, myricetin, kaemferol and (+) catechin and antioxidants in methyl linoleate. Journal of the Science of Food and Agriculture, 79, 499–506.
10.1002/(SICI)1097-0010(19990315)79:4<499::AID-JSFA204>3.0.CO;2-U CAS Web of Science® Google Scholar
- Piekut, J., Dec, D., & Zyskowska, A. (2016). Changes of the phenolic compounds content in chosen spice plants under the freezing process influence. Food Processing Technology Program, 1, 32–35.
- Ran, S., Kamboj, J. K., & Gandhi, V. (2011). Living life the natural way—Wheatgrass and health. Functional Foods in Health and Disease, 1(11), 444–456.
- Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 1231–1237.
- Reuveni, R., Shimoni, M., Karchi, Z., & Kuc, J. (1992). Peroxidase activity as a biochemical marker for resistance of muskmelon Cucumis melo to pseudoperospora cubensis. Phytopathology, 82, 749–753.
- Sánchez-Moreno, C., Plaza, L., de Ancos, B., & Cano, M. P. (2006). Nutritional characterisation of commercial traditional pasteurised tomato juices: Carotenoids, vitamin C and radical-scavenging capacity. Food Chemistry, 98(4), 749–756.
- Shakya, G., Balasubramanian, S., & Rajagopalan, R. (2015). Methanol extract of wheatgrass induces G1 cell cycle arrest in a p53-dependent manner and down regulates the expression of cyclin D1 in human laryngeal cancer cells-an in vitro and in silico approach. Pharmacognosy Magazine, 11(42), S139–S147.
- Shakya, G., Goud, C., Pajaniradje, S., & Rajagop, A. (2012). Protective role of wheat grass on oxidative stress in streptozotocin induced type 2 diabetic rats. International Journal of Pharmacy and Pharmaceutical Sciences, 4(3), 415–423.
- Sharma, S., Shrivastava, V. K., Shrivastav, A., & Shrivastav, B. R. (2013). Therapeutic potential of weathgrass (Triticum aestivum L.) for the treatment of chronic diseases. South Asian Journal of Experimental Biology, 3(6), 308–313.
- Singhal, V. K., Singhal, A. K., Jagatheesh, K., Padmavathi, K., Elangoran, N., Bangr, O. P., & Sil, R. (2012). Multifunctional role of green blood therapy to cure for many diseases. Chronicles of Young Scientists, 3, 12–16.
- Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology, 299, 152–178.
- Sliesaravičius, A., Pekarskas, J., Rutkovienė, V., & Baranauskis, K. (2006). Grain yield and disease resistance of winter cereal varieties and application of biological agent in organic agriculture. Agronomy Research, 4, 371–378.
- Teklić, T., Hancock, J. T., Engler, M., Paradicović, N., Cesar, V., Lepeduš, H., … Bešlo, D. (2008). Antioxidative responses in radish (Raphanus sativus L.) plants stressed by copper and lead in nutrient solution and soil. Acta Biologica Cracoviensia, 50(2), 79–86.
-
Tijskens, L. M. M.,
Barringer, S. A., &
Biekman, E. S. A. (2001). Modelling the change in colour of broccoli and green beans during blanching. Innovative Food Science and Emerging Technologies, 2, 303–313.
10.1016/S1466-8564(01)00045-5 Google Scholar
- Walters, R. (1992). The alternative cancer therapy book. New York: Avery Publishing Group.
- Wang, S. Y., & Lin, H. S. (2000). Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry, 48, 140.
- Wigmore, A. (1985). The wheatgrass book ( 1st ed.). New York, NY: Avery Publishing Group Inc.
- Yang, F., Basu, T. K., & Ooraikul, B. (2001). Studies on germination conditions and antioxidant content of wheatgrass. International Journal of Food Sciences and Nutrition, 52, 319–330.
- Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64, 555–559.