Preparation, Characterization and Antimicrobial Activity of Sodium Alginate Nanobiocomposite Films Incorporated with Ε-Polylysine and Cellulose Nanocrystals
Qiuye Tang
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Search for more papers by this authorCorresponding Author
Daodong Pan
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Nanjing Normal University, Jinling College, Department of Food Science and Nutrition, Nanjing, Jiangsu, 210097 China
Corresponding author. TEL: +86-(0)574-87608347; FAX: +86-(0)574-87608347; EMAIL: [email protected]Search for more papers by this authorYangying Sun
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Search for more papers by this authorJinxuan Cao
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Search for more papers by this authorYuxing Guo
Nanjing Normal University, Jinling College, Department of Food Science and Nutrition, Nanjing, Jiangsu, 210097 China
Search for more papers by this authorQiuye Tang
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Search for more papers by this authorCorresponding Author
Daodong Pan
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Nanjing Normal University, Jinling College, Department of Food Science and Nutrition, Nanjing, Jiangsu, 210097 China
Corresponding author. TEL: +86-(0)574-87608347; FAX: +86-(0)574-87608347; EMAIL: [email protected]Search for more papers by this authorYangying Sun
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Search for more papers by this authorJinxuan Cao
Ningbo University, Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo, Zhejiang, 315211 China
Search for more papers by this authorYuxing Guo
Nanjing Normal University, Jinling College, Department of Food Science and Nutrition, Nanjing, Jiangsu, 210097 China
Search for more papers by this authorAbstract
Ternary nanobiocomposite films based on sodium alginate (SA) with cellulose nanocrystals (CNC) derived from shaddock peel and ε-polylysine (ε-PL) have been prepared and characterized. The tensile strength and Young's modulus of nanobiocomposite films increased from 51.18 to 67.68 MPa and from 4.8 to 7.6 GPa upon CNC loading from 0.5 to 2.5 wt %. The positive effects of the addition of CNC in SA matrix were also confirmed by reductions in water vapor permeability (WVP). SA-based nanobiocomposites showed significant antibacterial activity against Escherichia coli and Staphylococcus aureus influenced by the ε-PL content. The films exhibited homogeneous and cohesive structures, indicating strong interactions between the filler and matrix. In addition, the good dispersion of CNC in the SA matrix could increase the stability of ε-PL and promote the diffusion of the antimicrobial agent from the films.
Practical Applications
Chilled duck is an important raw material for making the sauce duck, roast duck, favor duck and it has a very broad consumer market. The chilled duck stability during storage can be enhanced by film-coating as a prominent technique, and therefore an nanobiocomposite film based on sodium alginate (SA) incorporation with ε-polylysine (ε-PL) and cellulose nanocrystals (CNC) derived from shaddock peel were prepared in this study. The film-coating has a good antibacterial effect on preservation of chilled duck. This study will find a successful packing method for chilled duck and improve the shelf life.
References
- Abdollahi, M., Alboofetileh, M., Behrooz, R., Rezaei, M. and Miraki, R. 2013a. Reducing water sensitivity of alginate bio-nanocomposite film using cellulose nanoparticles. Int. J. Biol. Macromol. 54, 166–173.
- Abdollahi, M., Alboofetileh, M., Rezaei, M. and Behrooz, R. 2013b. Comparing physico-mechanical and thermal properties of alginate nanocomposite films reinforced with organic and/or inorganic nanofillers. Food Hydrocolloids 32, 416–424.
- Abdul Khalil, H. P. S., Bhat, A. H. and Ireana Yusra, A. F. 2012. Green composites from sustainable cellulose nanofibrils: A review. Carbohydr. Polym. 87, 963–979.
- Agulhon, P., Markova, V., Robitzer, M., Quignard, F. O. and Mineva, T. 2012. Structure of alginate gels: Interaction of diuronate units with divalent cations from density functional calculations. Biomacromolecules 13, 1899–1907.
- Akhtar, M. J., Jacquot, M., Arab-Tehrany, E., Gaani, C., Linder, M. and Desobry, S. 2010. Control of salmon oil photo-oxidation during storage in HPMC packaging film: Influence of film colour. Food Chem. 120, 395–401.
- Alves, J. S., dos Reis, K. C., Menezes, E. G., Pereira, F. V. and Pereira, J. 2015. Effect of cellulose nanocrystals and gelatin in corn starch plasticized films. Carbohydr. Polym. 115, 215–222.
- Benavides, S., Villalobos-Carvajal, R. and Reyes, J. 2012. Physical, mechanical and antibacterial properties of alginate film: Effect of the crosslinking degree and oregano essential oil concentration. J. Food Eng. 110, 232–239.
- Cai, L., Cao, A., Bai, F. and Li, J. 2015. Effect of ɛ-polylysine in combination with alginate coating treatment on physicochemical and microbial characteristics of Japanese sea bass (Lateolabrax japonicas) during refrigerated storage. LWT Food Sci. Technol. 62, 1053–1059.
- Chang, S. S., Lu, W. Y. W., Park, S. H. and Kang, D. H. 2010. Control of foodborne pathogens on ready-to-eat roast beef slurry by ɛ-polylysine. Int. J. Food Microbiol. 141, 236–241.
- Chang, Y., Mclandsborough, L. and Mcclements, D. J. 2014. Interaction of cationic antimicrobial (ɛ-polylysine) with food-grade biopolymers: Dextran, chitosan, carrageenan, alginate, and pectin. Food Res. Int. 64, 396–401.
- Cheng, Y., Lu, L., Zhang, W., Shi, J. and Cao, Y. 2012. Reinforced low density alginate-based aerogels: Preparation, hydrophobic modification and characterization. Carbohydr. Polym. 88, 1093–1099.
- Cho, M. J. and Park, B. D. 2011. Tensile and thermal properties of nanocellulose-reinforced poly (vinyl alcohol) nanocomposites. J. Ind. Eng. Chem. 17, 36–40.
- El Miri, N., Abdelouahdi, K., Barakat, A., Zahouily, M., Fihri, A., Solhy, A. and El Achaby, M. 2015. Bio-nanocomposite films reinforced with cellulose nanocrystals: Rheology of film-forming solutions, transparency, water vapor barrier and tensile properties of films. Carbohydr. Polym. 129, 156–167.
- Fortunati, E., Armentano, I., Zhou, Q., Iannoni, A., Saino, E., Visai, L., Berglund, L. A. and Kenny, J. M. 2012. Multifunctional bionanocomposite films of poly(lactic acid), cellulose nanocrystals and silver nanoparticles. Carbohydr. Polym. 87, 1596–1605.
- Gennadios, A., Weller, C. L. and Gooding, C. H. 1994. Measurement errors in water vapor permeability of highly permeable, hydrophilic edible films. J. Food Eng. 21, 395–409.
- Geornaras, I., Yoon, Y., Belk, K., Smith, G. and Sofos, J. 2007. Antimicrobial activity of ɛ-polylysine against Escherichia coli O157: H7, Salmonella typhimurium, and Listeria monocytogenes in various food extracts. J. Food Sci. 72, M330–M334.
- González, A. and Alvarez Igarzabal, C. I. 2015. Nanocrystal-reinforced soy protein films and their application as active packaging. Food Hydrocolloids 43, 777–784.
- Huq, T., Salmieri, S., Khan, A., Khan, R. A., Le Tien, C., Riedl, B., Fraschini, C., Bouchard, J., Uribe-Calderon, J., Kamal, M. R. and Lacroix, M. 2012. Nanocrystalline cellulose (NCC) reinforced alginate based biodegradable nanocomposite film. Carbohydr. Polym. 90, 1757–1763.
- Ionita, M., Pandele, M. A. and Iovu, H. 2013. Sodium alginate/graphene oxide composite films with enhanced thermal and mechanical properties. Carbohydr. Polym. 94, 339–344.
- Leal, D., Matsuhiro, B., Rossi, M. and Caruso, F. 2008. FT-IR spectra of alginic acid block fractions in three species of brown seaweeds. Carbohydr. Res. 343, 308–316.
- Lee, K. Y. and Mooney, D. J. 2012. Alginate: Properties and biomedical applications. Prog. Polym. Sci. 37, 106–126.
- Mouriño, V., Newby, P., Pishbin, F., Cattalini, J., Lucangioli, S. and Boccaccini, A. 2011. Physicochemical, biological and drug-release properties of gallium crosslinked alginate/nanoparticulate bioactive glass composite films. Soft Matter 7, 6705–6712.
- Papageorgiou, S. K., Kouvelos, E. P., Favvas, E. P., Sapalidis, A. A., Romanos, G. E. and Katsaros, F. K. 2010. Metal–carboxylate interactions in metal–alginate complexes studied with FTIR spectroscopy. Carbohydr. Res. 345, 469–473.
- Rocha, M., Loiko, M. R., Tondo, E. C. and Prentice, C. 2014. Physical, mechanical and antimicrobial properties of Argentine anchovy (Engraulis anchoita) protein films incorporated with organic acids. Food Hydrocolloids 37, 213–220.
- Savadekar, N., Karande, V., Vigneshwaran, N., Kadam, P. and Mhaske, S. 2015. Preparation of cotton linter nanowhiskers by high-pressure homogenization process and its application in thermoplastic starch. Appl. Nanosci. 5, 281–290.
- Shankar, S., Reddy, J. P., Rhim, J. W. and Kim, H. Y. 2015. Preparation, characterization, and antimicrobial activity of chitin nanofibrils reinforced carrageenan nanocomposite films. Carbohydr. Polym. 117, 468–475.
- Shih, L., Shen, M. H. and Van, Y. T. 2006. Microbial synthesis of poly (ɛ-lysine) and its various applications. Bioresour. Technol. 97, 1148–1159.
- Shima, S., Matsuoka, H., Iwamoto, T. and Sakai, H. 1984. Antimicrobial action of. EPSILON.-poly-L-lysine. J. Antibiotics 37, 1449–1455.
- Siqueira, G., Bras, J., Follain, N., Belbekhouche, S., Marais, S. and Dufresne, A. 2013. Thermal and mechanical properties of bio-nanocomposites reinforced by Luffa cylindrica cellulose nanocrystals. Carbohydr. Polym. 91, 711–717.
- Siripatrawan, U. and Harte, B. R. 2010. Physical properties and antioxidant activity of an active film from chitosan incorporated with green tea extract. Food Hydrocolloids 24, 770–775.
- Takahashi, H., Kashimura, M., Miya, S., Kuramoto, S., Koiso, H., Kuda, T. and Kimura, B. 2012. Effect of paired antimicrobial combinations on Listeria monocytogenes growth inhibition in ready-to-eat seafood products. Food Control 26, 397–400.
- Tam, S. K., Dusseault, J., Bilodeau, S., Langlois, G., Halle, J. P. and Yahia, L. 2011. Factors influencing alginate gel biocompatibility. J. Biomed. Mater. Res. A 98, 40–52.
- Teixeira, B., Marques, A., Pires, C., Ramos, C., Batista, I., Saraiva, J. A. and Nunes, M. L. 2014. Characterization of fish protein films incorporated with essential oils of clove, garlic and origanum: Physical, antioxidant and antibacterial properties. LWT Food Sci. Technol. 59, 533–539.
- Yadav, M., Rhee, K., Jung, I. and Park, S. 2013. Eco-friendly synthesis, characterization and properties of a sodium carboxymethyl cellulose/graphene oxide nanocomposite film. Cellulose 20, 687–698.
- Yadav, M., Rhee, K. Y. and Park, S. 2014. Synthesis and characterization of graphene oxide/carboxymethylcellulose/alginate composite blend films. Carbohydr. Polym. 110, 18–25.
- Yamanaka, K., Kito, N., Imokawa, Y., Maruyama, C., Utagawa, T. and Hamano, Y. 2010. Mechanism of ɛ-poly-L-lysine production and accumulation revealed by identification and analysis of an ɛ-poly-L-lysine-degrading enzyme. Appl. Environ. Microbiol. 76, 5669–5675.
- Yoshida, T. and Nagasawa, T. 2003. ɛ-Poly-L-lysine: Microbial production, biodegradation and application potential. Appl. Microbiol. Biotechnol. 62, 21–26.
- Zhang, L., Li, R., Dong, F., Tian, A., Li, Z. and Dai, Y. 2015. Physical, mechanical and antimicrobial properties of starch films incorporated with epsilon-poly-L-lysine. Food Chem. 166, 107–114.
