All crystal-based structural biology methods, including X-ray crystallography, serial synchrotron and serial femtosecond crystallography, and electron diffraction, require the preparation of biomolecular crystals. This article covers strategies for careful sample preparation in crystallization experiments to increase the chance of success.

The structure of a putative fucoidan-degrading glycoside hydrolase assigned to glycoside hydrolase family 168 reveals a (β/α)₈ fold. The catalytic machinery and potential substrate specificity were investigated through a structural comparison with a Fun168A oligosaccharide complex.

The crystal structure of S. maltophilia EntB revealed an unusual form of isochorismate lyase that is involved in siderophore biosynthesis.

Cryo-EM structures of M. tuberculosis imidazole glycerol phosphate dehydratase suggest that the enzyme exhibits a 24-mer assembly with 432 symmetry in both apo and small-molecule-bound states.

Sucrose phosphorylases catalyse a bi-bi reaction that interconverts sucrose and phosphate into glucose α-1-phosphate and fructose. Here, we present the first crystal structure of a sucrose phosphorylase from a marine organism.

Four crystal structures of rsCherry, where `rs' means reversible switchable, degraded under aerobic conditions provide valuable insights into oxygen-induced degradation in red fluorescent proteins.

This study presents the first fully resolved crystal structure of human fascin1, revealing its conformational plasticity and key interdomain interactions critical for actin bundling and cancer metastasis. These findings provide a structural foundation for the development of allosteric inhibitors and pave the way for time-resolved crystallographic studies to explore fascin1 dynamics in metastasis and drug targeting.