The cover image is based on the Original Article Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans by Meng-Chao Zhu et al., https://doi.org/10.1111/cpr.13477.

Developmental process of skin components, especially in the disparate dermis formation located on different part of the body.

scRNA-seq reveals three cell types in lens superficial tissue with two differentiation trajectories between them, and a cluster might be lens epithelial stem/progenitor cell.

Impairment of glutamine degradation and utilization in podocytes under diabetic kidney disease: LRH-1 positively regulates GlS2-mediated glutaminolysis, and downregulation of LRH-1 expression contributes to diabetes-induced disturbance of energy metabolism and injury in podocytes.

We transformed primed-derived hEPSCs into a new type of pluripotent stem cell AF9-hPSC with similar transcriptomic properties to human E8-E9 epiblasts by the addition of Activin A, bFGF and XAV939, which related to early human embryogenesis. AF9-hPSCs possessed differentiation capacity towards three germ layer lineages and primordial germ cells-like cells in vitro. In addition, AF9-hPSCs allowed trophectoderm lineage induction.

Persistent Atoh1 overexpression results in impaired stereocilia in both endogenous and regenerative hair cells (HCs). The enhanced expression of Espin optimizes the developmental process of stereocilia in Atoh1-induced HCs. The upregulation of Espin attenuates the damage to stereocilia caused by Atoh1 overexpression.

AS-IV prevented glucocorticoid-induced ANFH by promoting osteogenesis and angiogenesis via the Akt/Runx2 and Akt/HIF-1α/VEGF pathways, respectively, and suppressing apoptosis and oxidative stress via the Akt/Bad/Bcl-2 and Akt/Nrf2/HO-1 pathways, respectively.

To generate O-type Rh D-negative universal RBCs, an hiPSC line, HuAiPSC, which was derived from human umbilical arterial endothelial cells and showed haematopoietic development preference, was genetic modified to knockout RHD gene with CRISPR/Cas9. The modified seed cells, HuAiPSC-A1-RHD^−/− cells were induced towards erythroid cells with optimized protocol, which combined hypoxia and normoxia conditions for better erythrocyte outcome. Consequently, O-type Rh D-negative universal RBCs were efficiently generated, holding great potential in clinic applications.

An overview of model presented by this study. Pig induced pluripotent stem cells (iPSCs) reprogrammed using retrovirus or episomal vectors under the 3i/LAF culture medium could maintain long-term self-renewal and pluripotency without exogenous genes. Using episomal reprogramming system, rare pig integration-free iPSCs could be generated, which could be gene-edited and served as donor cells for cloning.

In the current study, the BA synthesis-related microbial signatures based on metagenome, the composition of the intestinal BAs pools based on metabolome, and the circulating FGF 19 level via ELISA were detected to characterize the profile of GM–BAs–FGF19 axis in patients with AF. Subsequently, the protective effect of FGF19 on palmitic acid-stimulated HL-1 cells in vitro was explored by evaluating the levels of lipid droplet accumulation, phosphorylated Yes-associated protein (YAP) and Ca²⁺/calmodulin-dependent protein kinases II (CaMKII) and secretion of interleukin-1 β (IL-1β) was revealed.

Haematopoietic stem cells (HSC) population in mouse bone marrow can be defined by the distinct expression levels of Spi1 and Gata1. By using a double fluorescence knock-in mouse model, PGdKI, in which the expression levels of PU.1 and GATA-1 are indicated by the expression of GFP and mCherry respectively, we uncover that the HSCs with lymphoid and myeloid repopulating activity are specifically enriched in a Lin⁻PU.1^dimGATA-1⁻ (LPG) cell subset. In vivo competitive repopulation assays demonstrate that bone marrow cells gated by LPG exhibit haematopoietic reconstitution activity which is comparable to that of classical Lin⁻Sca1⁺c-kit⁺ (LSK). The integrated analysis of single-cell RNA sequence data from LPG and LSK gated cells reveals that a transcriptional network governed by core TFs contributes to regulation of HSC multipotency.

With the process of oral submucous fibrosis, the expression level of Collagen increased with rising inflammatory response. In vitro experiments also demonstrate that IGF-1, the activator of Pi3k/Akt pathway promoted proliferation and migration of huvecs while LY294004, the inhibitor, exhibited the opposite function.

A subvacuum dressing can create a subvacuum environment in the area of the wound, and then calcium influx due to the opening of the mechanical calcium channel. Calcium influx leads to cytoskeleton depolymerisation through AKT1/PI3K signalling pathway. After cytoskeleton disaggregation, cells have stronger migration ability, thus promoting wound healing.