A PHYSIOLOGICAL INDICATOR TO ESTIMATE ALLICIN CONTENT IN GARLIC DURING STORAGE
ROXANA E. GONZÁLEZ
Laboratorio de Análisis de Residuos Tóxicos, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo. Almirante Brown 500, M5528 AHB, Chacras de Coria, Mendoza, Argentina
CONICET, Mendoza, Argentina
Search for more papers by this authorJOSÉ L. BURBA
INTA Estación Experimental La Consulta CC8 (5567), Mendoza, Argentina
Search for more papers by this authorCorresponding Author
ALEJANDRA B. CAMARGO
Laboratorio de Análisis de Residuos Tóxicos, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo. Almirante Brown 500, M5528 AHB, Chacras de Coria, Mendoza, Argentina
CONICET, Mendoza, Argentina
Instituto de Ciencias Básicas, Área Química (ICB), Universidad Nacional de Cuyo. Centro Universitario, Ciudad, Mendoza, Argentina
TEL: 54-261-4135010 int:1303; FAX: 54-261-4960469; EMAIL: [email protected]Search for more papers by this authorROXANA E. GONZÁLEZ
Laboratorio de Análisis de Residuos Tóxicos, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo. Almirante Brown 500, M5528 AHB, Chacras de Coria, Mendoza, Argentina
CONICET, Mendoza, Argentina
Search for more papers by this authorJOSÉ L. BURBA
INTA Estación Experimental La Consulta CC8 (5567), Mendoza, Argentina
Search for more papers by this authorCorresponding Author
ALEJANDRA B. CAMARGO
Laboratorio de Análisis de Residuos Tóxicos, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo. Almirante Brown 500, M5528 AHB, Chacras de Coria, Mendoza, Argentina
CONICET, Mendoza, Argentina
Instituto de Ciencias Básicas, Área Química (ICB), Universidad Nacional de Cuyo. Centro Universitario, Ciudad, Mendoza, Argentina
TEL: 54-261-4135010 int:1303; FAX: 54-261-4960469; EMAIL: [email protected]Search for more papers by this authorAbstract
ABSTRACT
The aim of the present work was to evaluate the application of a physiological indicator as marker of allicin content during postharvest storage at different temperatures. Sureño INTA garlic cultivar was stored at room temperature (20C ± 3) and cold storage (0C ± 0.5). Samples were taken periodically and allicin, pyruvate content were analyzed and the sprout growth was measured through of the visual index of dormancy (VID). The results showed that regardless of storage temperature, allicin, pyruvate content and VID changed significantly during the period of the assay. For the conditions evaluated, the highest allicin content was observed at 50% and 100% VID. These facts suggest that VID may be a suitable tool to estimate allicin level during storage.
PRACTICAL APLICATIONS
This work was addressed to resolve the problem of standardization of garlic subproducts according to their bioactive compounds levels. The information acquired is important for farmers and the food and phytotherapic industry in order to guarantee the adequate supply of garlic subproducts to the consumer. Therefore, we found that it is important to consider a physiological indicator as a tool to estimate the bioactive compound levels during postharvest storage.
REFERENCES
- AMAGASE, H., PETESCH, B., MATSUURA, H., KASUGA, S. and ITAKURA, Y. 2001. Intake of garlic and its bioactive components. Recent advances on the nutritional effects associated with the use of garlic as a supplement. J. Nutr. 131, 955s–962s.
- BAGHALIAN, K., ZIAI, S., NAGHAVI, M., BADI, H. and KHALIGHI, A. 2005. Evaluation of allicin content and botanical traits in Iranian garlic (Allium sativum L.) ecotypes. Sci. Hortic. 103, 155–166.
- BLOCK, E. 2010. The Aroma and Taste of Alliums: A Multitude of Flavor Precursors, in Garlic and Others Alliums. The Royal Society of Chemistry, Vol. 4 pp. 100–223, RBC Publishing, Science Park, Cambrige, U.K..
- BURBA, J. 1997. Panorama mundial y nacional de poblaciones y cultivares de ajo. Posibilidades de adaptación. 50 Temas sobre Producción de Ajo, Mendoza, Argentina, 2, 11–31.
- BURBA, J., CASALLI, V. and BUTELER, M. 1989. Intensidad de la dormición como parámetro fisiológico para agrupar cultivares de ajo (Allium sativum L.). Hortic. Argent. 8(18), 47–49.
- CAMARGO, A., MASUELLI, R. and BURBA, J. 2005. Characterization of argentine garlic cultivars for their allicin content. Acta Hort., (Liu Guangshu, ed.). Proceedings of the 4th International Symposium on Edible Alliaceae: Bejing, China, 688, 309–312.
- CAMARGO, A., MARCHEVSKY, E. and LUCO, J. 2007. QSAR study for the soybean 15-lipoxygenase inhibitory activity of organosulfur compounds derived from the essential oil of garlic. J. Agric. Food Chem. 55(8), 3096–3103.
- CAMARGO, A., RESNIZKY, S., MARCHEVSKY, E. and LUCO, J. 2010. Use of the Argentinean garlic (Allium sativum L.) germplasm mineral profile for determining geographic origin. J Food Compost. Anal. 23, 586–591.
- CAVAGNARO, P., CAMARGO, A., GALMARINI, C. and SIMON, P. 2007. Effect of cooking on garlic (Allium sativum L.) antiplatelet activity and thiosulfinates content. J. Agric. Food Chem. 55, 1280–1288.
- CECI, L. and CURZIO, O. 1992. Effects of irradiation and storage on the γ-glutamyl transpeptidase activity of garlic bulbs cv Red. J. Sci. Food Agric. 59, 505–510.
- DEBAENE, J., GOLDMAN, I. and YANDELL, B. 1999. Postharvest flux and genotype x environment effects for onion-induced antiplatelet activity, pungency, and soluble solids in long-day onion during postharvest cold storage. J. Am. Soc. Hortic. Sci. 124(4), 366–372.
- GOLDMAN, I., KOPELBERG, M., DEBAENE, J. and SCHWARTZ, B. 1996. Antiplatelet activity of onion (Allium cepa) is sulphur dependent. Thromb. Haemost. 76, 450–453.
- GONZÁLEZ, R., CAMARGO, A. and BURBA, J. 2007. Obtención de un estándar secundario de cuantificación. Síntesis y purificación de allicina. Rev. FCA Uncuyo 39(2), 61–70.
- GONZÁLEZ, R., SOTO, V., SANCE, M., CAMARGO, A. and GALMARINI, C. 2009. Variability of solids, organosulfur compounds, pungency and health-enhancing traits in garlic (Allium sativum L.) cultivars belonging to different ecophysiological groups. J. Agric. Food Chem. 57(21), 10282–10288.
- HUGHES, J., COLLIN, H., TREVOGA, A., TOMSETT, B., COSSTICK, R. and JONES, M. 2006. Effect of low storage temperature on some of the flavor precursors in garlic (Allium sativum). Plant Foods Hum. Nutr. 61, 81–85.
- ICHIKAWA, M., IDE, N. and ONO, K. 2006. Changes in organosulfur compounds in garlic cloves during storage. J. Agric. Food Chem. 54, 4849–4854.
- KOPSELL, D. and RANDLE, W. 1999. Changes in the S-alk(en)yl cysteine sulfoxides and their biosynthetic intermediates during onion storage. J. Am. Soc. Hortic. Sci. 124(2), 177–183.
- LANCASTER, J. and BOLAND, M. 1990. Flavour biochemistry. In Onions and Allied Crops, Vol. 3 ( J. Brewster and H. Rabinowitch, eds.) pp. 33–72, CRC. Press, Inc., Boca Raton, FL.
- LANZOTTI, V. 2006. The analysis of onion and garlic. J. Chromatogr. A 1112, 3–22.
- LAWSON, L., ZHEN, W. and HUGHES, B. 1991a. Identification and HPLC quantitation of the sulfides and dialk(en)yl thiosulfinates in commercial garlic products. Planta Med. 57, 363–370.
- LAWSON, L., WOOD, S. and HUGHES, B. 1991b. HPLC analysis of allicin and other thiosulfinates in garlic clove homogenates. Planta Med. 57, 263–270.
- LEDESMA, A., REATE, M., RACEA, R. and BURBA, J. 1980. Effect of low temperature and pre planting storage time on garlic clonal type Rosado Paraguya. Growth Phyton 39, 37–48.
- NATALE, P. and CAMARGO, A. 2005. Characterization of Argentine garlic cultivars by their pungency. Acta Hort., (Liu Guangshu, ed.). Proceedings of the 4th International Symposium on Edible Alliaceae: Bejing, China, 688, 313–315.
- PELLEGRINI, C., CROCI, C. and ORIOLI, G. 2000. Morphological changes induced by different doses of gamma irradiation in garlic sprouts. Radiat. Phys. Chem. 57, 315–318.
- PORTELA, J., BURBA, J., GABRIEL, E. and RIVERO, L. 2005. Argentine garlic II: The need of different cropping and postharvest management practices among cultivars. Acta Hort., (Liu Guangshu, ed.). Proceedings of the 4th International Symposium on Edible Alliaceae: Bejing, China, 688, 215–220.
-
RANDLE, W. and
LANCASTER, J.
2002. Sulphur compounds. Allium in relation to flavour quality. In Allium Crop Science: Recent Advances ( L. Currah, ed.) pp. 329–356, CABI, New York, NY.
10.1079/9780851995106.0329 Google Scholar
- SANCE, M., BAUZA, M., CAMARGO, A., GONZÁLEZ, R. and SOTO, V. 2006. Evaluation of the Argentinean garlic germplasm in relation to its aptitude for the freeze drying process. Mol. Med. Chem. 10, 33–34.
- SANCE, M., GONZÁLEZ, R., SOTO, V. and GALMARINI, C. 2008. Relationships between antiplatelet activity, dry matter content and flavor in onion cultivars. J. Food Agric. Environ. 6(3&4), 41–46.
- SOTO VARGAS, V., GONZÁLEZ, R., SANCE, M., CAMARGO, A. and BURBA, J. 2010. Efecto de la interacción genotipo-ambiente sobre la expresion del contenido de allicina y ácido pirúvico en ajo (Allium sativum L.). Rev. FCA Uncuyo 42(2), 15–22.
- TAPIERO, H., TOWNSEND, D. and TEW, K. 2004. Organosulfur compounds from Alliaceae in the prevention of human pathologies. Biomed. Pharmacother. 58, 183–193.
- VAZQUEZ-PRIETO, M., GONZÁLEZ, R., RENNA, N., GALMARINI, C. and MIATELLO, R. 2010. Aqueous garlic extracts prevent oxidative stress and vascular remodeling in an experimental model of metabolic syndrome. J. Agric. Food Chem. 58(11), 6630–6635.
- WORLD HEALTH ORGANIZATION (WHO) 2003a. Guidelines on good agricultural and collection practices (GACP) for medicinal plants. Geneva.
- WORLD HEALTH ORGANIZATION 2003b. Regional Office for South-East Asia Guidelines for the Regulation of Herbal Medicines in the South-East Asia Region. New Delhi SEA-Trad. Med.-82 Distribution: General Developed at the Regional Workshop on the Regulation of Herbal Medicines Bangkok. 24–26.