Volume 14, Issue 2 pp. 257-271
RESEARCH ARTICLE

Co-transplantation of Wharton's jelly mesenchymal stem cell-derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models

Marie Naudot

Marie Naudot

EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France

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Anaïs Barre

Anaïs Barre

EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France

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Alexandre Caula

Alexandre Caula

Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France

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Henri Sevestre

Henri Sevestre

Service d'anatomie et de cytology pathologique, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France

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Stéphanie Dakpé

Stéphanie Dakpé

EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France

Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France

Institut Faire Faces, Amiens, France

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Andreas Albert Mueller

Andreas Albert Mueller

Department of Cranio-Maxillofacial Surgery, University and University Hospital Basel, Basel, Switzerland

Department of Biomedical Engineering, Regenerative Medicine and Oral Health Technologies, University of Basel, Allschwil, Switzerland

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Bernard Devauchelle

Bernard Devauchelle

EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France

Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France

Institut Faire Faces, Amiens, France

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Sylvie Testelin

Sylvie Testelin

EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France

Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France

Institut Faire Faces, Amiens, France

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Jean Pierre Marolleau

Jean Pierre Marolleau

Service d'Hématologie Clinique, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France

EA 4666, HEMATIM, University of Picardie Jules Verne, Amiens, France

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Sophie Le Ricousse

Corresponding Author

Sophie Le Ricousse

EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France

Institut Faire Faces, Amiens, France

Correspondence

Sophie Le Ricousse, EA 7516, CHIMERE, Centre Universitaire de Recherche en Santé, CHU Amiens Picardie Sud, F-80054 Amiens cedex 1, France.

Email: [email protected]

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First published: 11 November 2019
Citations: 5
Marie Naudot, Anaïs Barre, Jean Pierre Marolleau, and Sophie Le Ricousse contributed equally to the work presented in this manuscript.

Abstract

A major challenge in bone tissue engineering is the lack of post-implantation vascular growth into biomaterials. In the skeletal system, blood vessel growth appears to be coupled to osteogenesis—suggesting the existence of molecular crosstalk between endothelial cells (ECs) and osteoblastic cells. The present study (performed in two murine ectopic models) was designed to determine whether co-transplantation of human Wharton's jelly mesenchymal stem cell-derived osteoblasts (WJMSC-OBs) and human differentiated ECs enhances bone regeneration and stimulates angiogenesis, relative to the seeding of WJMSC-OBs alone.

Human WJMSC-OBs and human ECs were loaded into a silicate-substituted calcium phosphate (SiCaP) scaffold and then ectopically implanted at subcutaneous or intramuscular sites in nude mice. At both subcutaneous and intramuscular implantation sites, we observed ectopic bone formation and osteoids composed of host cells when WJMSC-OBs were seeded into the scaffold. However, the addition of ECs was associated with a lower level of osteogenesis, and we did not observe stimulation of blood vessel ingrowth. in vitro studies demonstrated that WJMSC-OBs lost their ability to secrete vascular endothelial growth factor and stromal cell-derived factor 1—including when ECs were present. In these two murine ectopic models, our cell-matrix environment combination did not seem to be optimal for inducing vascularized bone reconstruction.

CONFLICT OF INTEREST

The authors declare that they have no competing or commercial interests.

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