Co-transplantation of Wharton's jelly mesenchymal stem cell-derived osteoblasts with differentiated endothelial cells does not stimulate blood vessel and osteoid formation in nude mice models
Marie Naudot
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Search for more papers by this authorAnaïs Barre
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Search for more papers by this authorAlexandre Caula
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Search for more papers by this authorHenri Sevestre
Service d'anatomie et de cytology pathologique, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Search for more papers by this authorStéphanie Dakpé
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Institut Faire Faces, Amiens, France
Search for more papers by this authorAndreas Albert Mueller
Department of Cranio-Maxillofacial Surgery, University and University Hospital Basel, Basel, Switzerland
Department of Biomedical Engineering, Regenerative Medicine and Oral Health Technologies, University of Basel, Allschwil, Switzerland
Search for more papers by this authorBernard Devauchelle
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Institut Faire Faces, Amiens, France
Search for more papers by this authorSylvie Testelin
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Institut Faire Faces, Amiens, France
Search for more papers by this authorJean Pierre Marolleau
Service d'Hématologie Clinique, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
EA 4666, HEMATIM, University of Picardie Jules Verne, Amiens, France
Search for more papers by this authorCorresponding Author
Sophie Le Ricousse
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Institut Faire Faces, Amiens, France
Correspondence
Sophie Le Ricousse, EA 7516, CHIMERE, Centre Universitaire de Recherche en Santé, CHU Amiens Picardie Sud, F-80054 Amiens cedex 1, France.
Email: [email protected]
Search for more papers by this authorMarie Naudot
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Search for more papers by this authorAnaïs Barre
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Search for more papers by this authorAlexandre Caula
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Search for more papers by this authorHenri Sevestre
Service d'anatomie et de cytology pathologique, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Search for more papers by this authorStéphanie Dakpé
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Institut Faire Faces, Amiens, France
Search for more papers by this authorAndreas Albert Mueller
Department of Cranio-Maxillofacial Surgery, University and University Hospital Basel, Basel, Switzerland
Department of Biomedical Engineering, Regenerative Medicine and Oral Health Technologies, University of Basel, Allschwil, Switzerland
Search for more papers by this authorBernard Devauchelle
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Institut Faire Faces, Amiens, France
Search for more papers by this authorSylvie Testelin
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Service de chirurgie maxillo-faciale, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
Institut Faire Faces, Amiens, France
Search for more papers by this authorJean Pierre Marolleau
Service d'Hématologie Clinique, Centre Hospitalier Universitaire Amiens Picardie, Amiens, France
EA 4666, HEMATIM, University of Picardie Jules Verne, Amiens, France
Search for more papers by this authorCorresponding Author
Sophie Le Ricousse
EA 7516, CHIMERE, University of Picardie Jules Verne, Amiens, France
Institut Faire Faces, Amiens, France
Correspondence
Sophie Le Ricousse, EA 7516, CHIMERE, Centre Universitaire de Recherche en Santé, CHU Amiens Picardie Sud, F-80054 Amiens cedex 1, France.
Email: [email protected]
Search for more papers by this authorAbstract
A major challenge in bone tissue engineering is the lack of post-implantation vascular growth into biomaterials. In the skeletal system, blood vessel growth appears to be coupled to osteogenesis—suggesting the existence of molecular crosstalk between endothelial cells (ECs) and osteoblastic cells. The present study (performed in two murine ectopic models) was designed to determine whether co-transplantation of human Wharton's jelly mesenchymal stem cell-derived osteoblasts (WJMSC-OBs) and human differentiated ECs enhances bone regeneration and stimulates angiogenesis, relative to the seeding of WJMSC-OBs alone.
Human WJMSC-OBs and human ECs were loaded into a silicate-substituted calcium phosphate (SiCaP) scaffold and then ectopically implanted at subcutaneous or intramuscular sites in nude mice. At both subcutaneous and intramuscular implantation sites, we observed ectopic bone formation and osteoids composed of host cells when WJMSC-OBs were seeded into the scaffold. However, the addition of ECs was associated with a lower level of osteogenesis, and we did not observe stimulation of blood vessel ingrowth. in vitro studies demonstrated that WJMSC-OBs lost their ability to secrete vascular endothelial growth factor and stromal cell-derived factor 1—including when ECs were present. In these two murine ectopic models, our cell-matrix environment combination did not seem to be optimal for inducing vascularized bone reconstruction.
CONFLICT OF INTEREST
The authors declare that they have no competing or commercial interests.
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