Bio-Coreactant-Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport
Dr. Cheng Ma
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorShaojun Wu
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorDr. Yang Zhou
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164 USA
These authors contributed equally to this work.
Search for more papers by this authorHui-Fang Wei
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorProf. Dr. Jianrong Zhang
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorCorresponding Author
Dr. Zixuan Chen
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorProf. Dr. Jun-Jie Zhu
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorCorresponding Author
Prof. Dr. Yuehe Lin
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164 USA
Search for more papers by this authorCorresponding Author
Dr. Wenlei Zhu
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164 USA
Search for more papers by this authorDr. Cheng Ma
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorShaojun Wu
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
These authors contributed equally to this work.
Search for more papers by this authorDr. Yang Zhou
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164 USA
These authors contributed equally to this work.
Search for more papers by this authorHui-Fang Wei
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorProf. Dr. Jianrong Zhang
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorCorresponding Author
Dr. Zixuan Chen
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorProf. Dr. Jun-Jie Zhu
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China
Search for more papers by this authorCorresponding Author
Prof. Dr. Yuehe Lin
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164 USA
Search for more papers by this authorCorresponding Author
Dr. Wenlei Zhu
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164 USA
Search for more papers by this authorAbstract
A bio-coreactant-enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)32+ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time-resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)33+ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single-cell studies, the bio-coreactant-enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR-1.
Conflict of interest
The authors declare no conflict of interest.
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