Biosynthesis of Isoprene Units: Mössbauer Spectroscopy of Substrate and Inhibitor Binding to the [4Fe-4S] Cluster of the LytB/IspH Enzyme†
Annegret Ahrens-Botzong
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern (Germany)
Search for more papers by this authorDr. Karnjapan Janthawornpong
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Search for more papers by this authorDr. Juliusz A. Wolny
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern (Germany)
Search for more papers by this authorDr. Erasmienne Ngouamegne Tambou
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Search for more papers by this authorProf. Michel Rohmer
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Search for more papers by this authorDr. Sergiy Krasutsky
Department of Chemistry, University of Utah, 315 South 1400 East RM 2020, Salt Lake City, Utah 84112 (USA)
Search for more papers by this authorProf. C. Dale Poulter
Department of Chemistry, University of Utah, 315 South 1400 East RM 2020, Salt Lake City, Utah 84112 (USA)
Search for more papers by this authorProf. Volker Schünemann
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern (Germany)
Search for more papers by this authorCorresponding Author
Dr. Myriam Seemann
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)Search for more papers by this authorAnnegret Ahrens-Botzong
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern (Germany)
Search for more papers by this authorDr. Karnjapan Janthawornpong
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Search for more papers by this authorDr. Juliusz A. Wolny
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern (Germany)
Search for more papers by this authorDr. Erasmienne Ngouamegne Tambou
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Search for more papers by this authorProf. Michel Rohmer
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Search for more papers by this authorDr. Sergiy Krasutsky
Department of Chemistry, University of Utah, 315 South 1400 East RM 2020, Salt Lake City, Utah 84112 (USA)
Search for more papers by this authorProf. C. Dale Poulter
Department of Chemistry, University of Utah, 315 South 1400 East RM 2020, Salt Lake City, Utah 84112 (USA)
Search for more papers by this authorProf. Volker Schünemann
Fachbereich Physik, University of Kaiserslautern, Erwin-Schrödinger-Strasse 46, 67653 Kaiserslautern (Germany)
Search for more papers by this authorCorresponding Author
Dr. Myriam Seemann
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)
Université de Strasbourg/CNRS, UMR 7177, Institut Le Bel, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg Cedex (France)Search for more papers by this authorWe are grateful to Prof. A. Boronat (University of Barcelona, Spain) and his group for providing us with the E. coli strain overexpressing LytB. We thank M. Parisse for technical assistance. This work was supported by the “Agence Nationale de la Recherche” (ANR-05-JCJC-0177-01) to M.S., by the Thai government to K.J., by NIH grant GM25521 to C.D.P., and by the German Federal Ministry of Education and Research (05K10UKA) and NANOKAT to V.S.
Graphical Abstract
Ein faszinierender Kubus: LytB, ein Enzym mit einem [4Fe-4S]-Cluster, katalysiert den letzten Schritt des Methylerythritolphosphatweges, eines Targets für antibakterielle und antiparasitäre Wirkstoffe. Feldabhängige Mößbauer-Spektroskopie zeigte, dass das besondere vierte Eisenatom des [4Fe-4S]-Clusters an die Hydroxygruppe des Substrats (siehe Bild) sowie die Amin- und die Thiolfunktion der beiden Substratanaloga bindet, die gute Inhibitoren sind.
Supporting Information
Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors.
| Filename | Description |
|---|---|
| ange_201104562_sm_lytb_amino_2.pdb574.9 KB | lytb_amino_2 |
| ange_201104562_sm_lytb_cluster_amino_2.pdb6.2 KB | lytb_cluster_amino_2 |
| ange_201104562_sm_lytb_cluster_hmbpp_1.pdb4.9 KB | lytb_cluster_hmbpp_1 |
| ange_201104562_sm_lytb_cluster_thiol_3.pdb4.8 KB | lytb_cluster_thiol_3 |
| ange_201104562_sm_lytb_hmbpp_1.pdb571.1 KB | lytb_hmbpp_1 |
| ange_201104562_sm_lytb_thiol_3.pdb570.9 KB | lytb_thiol_3 |
| ange_201104562_sm_miscellaneous_information.pdf2 MB | miscellaneous_information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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