This chapter contains sections titled:

• Introduction

• LC-MS Overview

  • Optimization

    • Modification of Mobile Phases and Ionization Parameters

    • HPLC Versus UPLC

    • Fluorinated HPLC Solid Phases

    • Reduction of Ion Suppression

• Clinical Chemistry and Forensic Applications

  • Extraction and Analytical Considerations

  • Forensic Detection of Plant-derived Alkaloids

    • Plant-associated Intoxications

    • Illicit Drug Use: Multiple Reaction Monitoring

    • Quality Control of Herbal Preparations: APCI-MS

• Metabolite Profiling and Structure Determination

  • LC-MS/MS Approaches to the Identification/Structural Elucidation of Alkaloid Drug Metabolites

    • Tandem MS

    • Accurate Mass Measurement

    • Chemical Modification

  • Minimization of Sample Treatment

  • Structure Determination

    • Nudicaulins from Papaver nudicaule: High-resolution MS

    • Endophyte Alkaloids: An MS Fragment Marker

• Pyrrolizidine Alkaloids and Their N-Oxides

  • Solid Phase Extraction

  • Qualitative Profiling

    • Echium plantagineum and Echium vulgare

    • Senecio ovatus and Senecio jacobaea

  • Quantitative Analysis

    • Calibration Standards

    • Honey

• Alkaloids from Delphinium spp. (Larkspurs)

  • Flow Injection (FI) Mass Spectrometry

    • Qualitative FI Analysis

    • Quantitative FI Analyses

    • Chemotaxonomy of Delphinium Species

  • LC-MS Analysis of Diterpene Alkaloids

    • Toxicokinetics and Clearance Times

    • Diagnosis of Poisoning

  • Structural Elucidation of Norditerpenoid Alkaloids

    • Stereochemical Indications

    • Isomeric Differentiation Using Tandem Mass Spectrometry

    • Novel Diterpene Alkaloid Identification: Application of Tandem Mass Spectrometry

• Conclusions