ORIGINAL ARTICLE
Effect of quercetin and Abelmoschus esculentus (L.) Moench on lipids metabolism and blood glucose through AMPK-α in diabetic rats (HFD/STZ)
Zohreh Nasrollahi,
Kahin ShahaniPour,
Ramesh Monajemi,
Ali Mohammad Ahadi,
Zohreh Nasrollahi
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Search for more papers by this authorCorresponding Author
Kahin ShahaniPour
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Correspondence
Kahin ShahaniPour, Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Email: [email protected]
Search for more papers by this authorRamesh Monajemi
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Search for more papers by this authorAli Mohammad Ahadi
Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran
Search for more papers by this authorZohreh Nasrollahi,
Kahin ShahaniPour,
Ramesh Monajemi,
Ali Mohammad Ahadi,
Zohreh Nasrollahi
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Search for more papers by this authorCorresponding Author
Kahin ShahaniPour
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Correspondence
Kahin ShahaniPour, Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Email: [email protected]
Search for more papers by this authorRamesh Monajemi
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Search for more papers by this authorAli Mohammad Ahadi
Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran
Search for more papers by this authorAbstract
Phosphoenolpyruvate carboxykinase (PEPCK) is a key enzyme in the glyconeogenesis pathway. The AMP-activated protein kinase alpha (AMPK-α) pathway regulates PEPCK, which itself is activated by the AMP/ATP ratio and liver kinase B1 (KB1). The Abelmoschus esculentus (L.) Moench (okra) plant contains a large amount of quercetin that can function as an agonist or an antagonist. The aim of this study was to examine the effects of quercetin flavonoid and A. esculentus extract on the level of AMPK-α expression and associated metabolic pathways. The findings demonstrate that metformin, quercetin, and okra extract may significantly raise AMPK-α levels while significantly lowering PEPCK and hormone-sensitive lipase (HSL) levels, in addition to improving glucose and lipid profiles. By stimulating KB1, these substances increased AMPK-α activation. Additionally, AMPK-α activation improved insulin resistance and Glucose transporter type 4 (GLUT4) gene expression levels. Since AMPK-α maintains energy balance and its activity has not been reported to be inhibited so far, it could be a potent therapeutic target.
Practical applications
The development of effective AMPK-α agonists and antagonists holds promise for the treatment of metabolic disorders like diabetes. Dietary polyphenols are a valuable source for developing new drugs. However, due to the lack of understanding of the underlying mechanisms of their effect on cells, their use in the treatment of diabetes is controversial. In addition to chemicals that have medicinal benefits, chemists are searching for less harmful substances. Using plants containing bioactive chemicals for this purpose can be a good alternative to chemical drugs.
CONFLICT OF INTEREST
The authors have no conflict of interest to declare.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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