Pitaya fruit extract ameliorates the healthspan on copper-induced toxicity of Caenorhabditis elegans
Wagner Antonio Tamagno
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Conceptualization, Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorWallace Santini
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorAmanda dos Santos
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorCarla Alves
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Graduate Program in Bioexperimentation, Universidade de Passo Fundo, São José, Passo Fundo, Brazil
Contribution: Formal analysis, Methodology
Search for more papers by this authorDenise Bilibio
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorNathália Tafarel Sutorillo
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorDaniele Coradini Zamberlan
Biochemistry and Molecular Biology Department, Center of Natural and Exacts Science, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Contribution: Writing - review & editing
Search for more papers by this authorRosilene Rodrigues Kaizer
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Graduate Program in Environmental Science and Technology, Federal University of Fronteira Sul (UFFS) – Erechim Campus, Erechim, Rio Grande do Sul, Brazil
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration
Search for more papers by this authorCorresponding Author
Leonardo José Gil Barcellos
Graduate Program in Bioexperimentation, Universidade de Passo Fundo, São José, Passo Fundo, Brazil
Graduate Program in Pharmacology, Universidade Federal de Santa Maria, Cidade Universitária, Camobi, Santa Maria, Brazil
Correspondence
Leonardo José Gil Barcellos, Graduate Program in Bioexperimentation and Graduate Program in Environmental Science, Universidade de Passo Fundo, BR 285, São José, Passo Fundo, RS 99052-900, Brazil.
Email: [email protected]
Contribution: Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorWagner Antonio Tamagno
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Conceptualization, Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorWallace Santini
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorAmanda dos Santos
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorCarla Alves
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Graduate Program in Bioexperimentation, Universidade de Passo Fundo, São José, Passo Fundo, Brazil
Contribution: Formal analysis, Methodology
Search for more papers by this authorDenise Bilibio
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorNathália Tafarel Sutorillo
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Contribution: Methodology
Search for more papers by this authorDaniele Coradini Zamberlan
Biochemistry and Molecular Biology Department, Center of Natural and Exacts Science, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Contribution: Writing - review & editing
Search for more papers by this authorRosilene Rodrigues Kaizer
Biochemistry and Molecular Biology Laboratory, Federal Institute of Education, Science, and Technology of Rio Grande do Sul, Sertão, Rio Grande do Sul, Brazil
Graduate Program in Environmental Science and Technology, Federal University of Fronteira Sul (UFFS) – Erechim Campus, Erechim, Rio Grande do Sul, Brazil
Contribution: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration
Search for more papers by this authorCorresponding Author
Leonardo José Gil Barcellos
Graduate Program in Bioexperimentation, Universidade de Passo Fundo, São José, Passo Fundo, Brazil
Graduate Program in Pharmacology, Universidade Federal de Santa Maria, Cidade Universitária, Camobi, Santa Maria, Brazil
Correspondence
Leonardo José Gil Barcellos, Graduate Program in Bioexperimentation and Graduate Program in Environmental Science, Universidade de Passo Fundo, BR 285, São José, Passo Fundo, RS 99052-900, Brazil.
Email: [email protected]
Contribution: Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorFunding information
This research was supported by the National Council for Scientific and Technological Development (CNPq), the Research Support Foundation of the State of Rio Grande do Sul (FAPERGS), and the Federal Institute of Rio Grande do Sul – Campus Sertão (IFRS). LJGB is supported by grants from the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS) 19/2551-0001-873-8 and holds a research fellowship of the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) 303263/2018-0.
Abstract
Copper (Cu) is an essential metal and it is important for metabolism. However, in high concentrations, it becomes toxic. Metal-induced toxicity is the cause of many neurodegenerative diseases. So it is necessary to search mechanisms to find ways of healthy aging. Natural compounds and diets based on fruits are increasingly common and could lead to a healthy life. Pitaya (Hylocereus undatus) is a tropical and Latin American, fruit that is gaining more popularity due to its antioxidant properties. Here, we evaluate the preventive and curative effect of different doses of microencapsulated pulp H. undatus extract on copper-induced toxicity. For this we use the nematode Caenorhabditis elegans, to investigate the effects of pitaya extract on behavior, lipid peroxidation, antioxidant chaperon, and cholinergic nervous system (ColNS). Results showed behavioral changes, decreased cell death biomarkers, and lipid peroxidation caused by copper, and these toxic effects were prevented and reverted by Pitaya's extract. After all, the extract can be used in diet as a supplement and studied to treat or prevent specific diseases, some of them linked to contamination and senility-related conditions.
Practical applications
This research has been aimed to provide the uses of Hylocereus undatus microencapsulated pulp extract for the prevention and treatment of copper-induced toxicity. We have been shown that Pitaya is a good source of antioxidant compounds that can ameliorate the antioxidant system as well as the cholinergic nervous system avoiding behavior changes before and after the metal toxicity of copper. Therefore, the potential applications and common use of this extract can serve as food supplementation to prevent metal oxidative damage as well as to repair clinical cases of copper poisoning.
CONFLICT OF INTEREST
The authors declare that they have no competing interests.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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