Volume 45, Issue 12 e13964
ORIGINAL ARTICLE

Sialoglycoproteins isolated from the eggs of Carassius auratus alleviates CCL4-induced liver injury via downregulation of the IRE-α/NF-κB signaling pathway

Hao Yue

Hao Yue

College of Food Science and Engineering, Ocean University of China, Qingdao, China

Contribution: Data curation, Writing - original draft

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Peng Wang

Peng Wang

College of Food Science and Engineering, Ocean University of China, Qingdao, China

Contribution: Data curation, Validation, Visualization

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Lei Zhang

Lei Zhang

College of Food Science and Engineering, Ocean University of China, Qingdao, China

Contribution: Visualization

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Ding Ning

Ding Ning

Malvern College Qingdao, Qingdao, China

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Weizhen Cai

Weizhen Cai

College of Food Science and Engineering, Ocean University of China, Qingdao, China

Contribution: Software, Visualization

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Yanchao Wang

Corresponding Author

Yanchao Wang

College of Food Science and Engineering, Ocean University of China, Qingdao, China

Correspondence

Jingfeng Wang and Yanchao Wang, College of Food Science and Engineering, Ocean University of China, Qingdao, China.

Email: [email protected] (J. W.); [email protected] (Y. W.)

Contribution: Conceptualization, Supervision, Writing - review & editing

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Jingfeng Wang

Corresponding Author

Jingfeng Wang

College of Food Science and Engineering, Ocean University of China, Qingdao, China

Correspondence

Jingfeng Wang and Yanchao Wang, College of Food Science and Engineering, Ocean University of China, Qingdao, China.

Email: [email protected] (J. W.); [email protected] (Y. W.)

Contribution: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing - review & editing

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First published: 03 November 2021
Citations: 3

Abstract

Chemical liver injury is a common cause of liver disease primarily characterized by oxidative stress and inflammation. Sialoglycoproteins isolated from the eggs of Carassius auratus (Ca-SGP) have been proved to exhibit the antioxidant effect. However, the effect of Ca-SGP on liver injury remains unclear. Thus, this study was aimed to determine the effect of Ca-SGP on CCL4-induced chronic chemical liver injury and explore the underlying molecular mechanism. Results showed that Ca-SGP mitigated the elevated levels of serum alanine aminotransferase and aspartate aminotransferase, inhibited the systemic oxidative stress, and reduced the levels of pro-inflammatory factors TNF-α and IL-1β. Histologic results showed that Ca-SGP supplements alleviated hepatocyte necrosis and liver macrophage infiltration. Further, Ca-SGP supplement decreased endoplasmic reticulum stress-related proteins expression, including BiP, IRE-α, p-IRE-α, and TRAF2, and further inhibited the trigger of the NF-κB pathway. In summary, Ca-SGP might be a novel agent for liver injury treatment, and its potential mechanism was related to the inhibition of liver inflammation induced by the endoplasmic reticulum.

Practical application

The fish egg is an important by-product in fish processing. Carassius auratus is a common freshwater fish with large catches and low prices. However, the eggs of C. auratus are usually direct discard or processed into salted roe products, and the quality and value of these salted products are unsatisfactory. In this current study, we confirmed that sialoglycoproteins isolated from the C. auratus eggs have the potential for the treatment of liver injury and determined that its mechanism is related to the endoplasmic reticulum and inflammation, which put forward a new idea for solving the by-product of fish processing.

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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