Tendon-derived stem/progenitor cell aging: defective self-renewal and altered fate
Zuping Zhou
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorTakintope Akinbiyi
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorLili Xu
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorMelissa Ramcharan
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorDaniel J. Leong
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorStephen J. Ros
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorAlexis C. Colvin
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorMitchell B. Schaffler
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorRobert J. Majeska
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorEvan L. Flatow
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorHui B. Sun
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorZuping Zhou
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorTakintope Akinbiyi
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorLili Xu
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorMelissa Ramcharan
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorDaniel J. Leong
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorStephen J. Ros
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorAlexis C. Colvin
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorMitchell B. Schaffler
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorRobert J. Majeska
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorEvan L. Flatow
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorHui B. Sun
Department of Orthopaedics, Mount Sinai School of Medicine, New York, NY, USA
Search for more papers by this authorSummary
Aging is a major risk factor for tendon injury and impaired tendon healing, but the basis for these relationships remains poorly understood. Here we show that rat tendon-derived stem/progenitor cells (TSPCs) differ in both self-renewal and differentiation capability with age. The frequency of TSPCs in tendon tissues of aged animals is markedly reduced based on colony formation assays. Proliferation rate is decreased, cell cycle progression is delayed and cell fate patterns are also altered in aged TSPCs. In particular, expression of tendon lineage marker genes is reduced while adipocytic differentiation increased. Cited2, a multi-stimuli responsive transactivator involved in cell growth and senescence, is also downregulated in aged TSPCs while CD44, a matrix assembling and organizing protein implicated in tendon healing, is upregulated, suggesting that these genes participate in the control of TSPC function.
Supporting Information
Fig. S1 Expression of stem cell markers is similar and widespread in both young and aged TSPCs.
Fig. S2 Osteogenic and chondrogenic differentiation potentials of young and aged TSPCs in vitro.
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