Synthesis and Application of FRET Nanoparticles in the Profiling of a Protease†
Lionel Marcon
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorCorresponding Author
Corentin Spriet
Biophotonique cellulaire fonctionnelle Institut de Recherche Interdisciplinaire Université des Sciences et Technologies de Lille USR 3078 CNRS, Parc de la Haute Borne, 50 avenue de Halley 59658 Villeneuve d'Ascq (France)
Biophotonique cellulaire fonctionnelle Institut de Recherche Interdisciplinaire Université des Sciences et Technologies de Lille USR 3078 CNRS, Parc de la Haute Borne, 50 avenue de Halley 59658 Villeneuve d'Ascq (France).Search for more papers by this authorTimothy D. Meehan
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorBronwyn J. Battersby
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorGwendolyn A. Lawrie
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorLaurent Héliot
Biophotonique cellulaire fonctionnelle Institut de Recherche Interdisciplinaire Université des Sciences et Technologies de Lille USR 3078 CNRS, Parc de la Haute Borne, 50 avenue de Halley 59658 Villeneuve d'Ascq (France)
Search for more papers by this authorMatt Trau
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorLionel Marcon
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorCorresponding Author
Corentin Spriet
Biophotonique cellulaire fonctionnelle Institut de Recherche Interdisciplinaire Université des Sciences et Technologies de Lille USR 3078 CNRS, Parc de la Haute Borne, 50 avenue de Halley 59658 Villeneuve d'Ascq (France)
Biophotonique cellulaire fonctionnelle Institut de Recherche Interdisciplinaire Université des Sciences et Technologies de Lille USR 3078 CNRS, Parc de la Haute Borne, 50 avenue de Halley 59658 Villeneuve d'Ascq (France).Search for more papers by this authorTimothy D. Meehan
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorBronwyn J. Battersby
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorGwendolyn A. Lawrie
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorLaurent Héliot
Biophotonique cellulaire fonctionnelle Institut de Recherche Interdisciplinaire Université des Sciences et Technologies de Lille USR 3078 CNRS, Parc de la Haute Borne, 50 avenue de Halley 59658 Villeneuve d'Ascq (France)
Search for more papers by this authorMatt Trau
Centre for Nanotechnology and Biomaterials Australian Institute for Bioengineering and Nanotechnology University of Queensland, St. Lucia, QLD 4072 (Australia)
Search for more papers by this authorRecombinant WNV protease was kindly donated by Dr. Paul Young (School of Molecular and Microbial Sciences, University of Queensland, Australia). The authors acknowledge Damien Schapman and the Biophotonic Core Facility of Lille (USR3078/IRI). This work was supported by the ARC (FF0455861; DP0555914), the ANR (2007 PFTV 01101), and the NHMRC (301267). We acknowledge the Centre for Microscopy and Microanalysis (UQ) for the use of electron microscopy equipment. The authors L. Marcon and C. Spriet had an equal contribution.
Graphical Abstract
Fluorescent silica nanoparticles incorporating unique ratios of energy-transfer dyes are synthesized and applied as colloidal barcodes to encode a microsphere-bound combinatorial peptide library. The affinity of the West Nile virus protease is profiled using this library with cleavage of the peptide detected by flow cytometry. The cleaved peptide substrates are sorted and then identified through decoding by confocal microscopy combined with spectral unmixing (see image).
Supporting Information
Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors.
| Filename | Description |
|---|---|
| smll_200801887_sm_suppdata.pdf224.4 KB | suppdata |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1 H. M. Geysen, F. Schoenen, D. Wagner, R. Wagner, Nat. Rev. Drug Discovery 2003, 2, 222.
- 2 O. H. Aina, R. Liu, J. L. Sutcliffe, J. Marik, C. X. Pan, K. S. Lam, Mol. Pharmaceutics 2007, 4, 631.
- 3 O. Schilling, C. M. Overall, Nat. Biotechnol. 2008, 26, 685.
- 4 K. S. Lam, M. Lebl, V. Krchnak, Chem. Rev. 1997, 97, 411.
- 5 A. W. Czarnik, Curr. Opin. Chem. Biol. 1997, 1, 60.
- 6 B. J. Egner, S. Rana, H. Smith, N. Bouloc, J. G. Frey, W. S. Brocklesby, M. Bradley, Chem. Commun. 1997, 735.
- 7 L. Marcon, D. Kozak, B. J. Battersby, K. J. Chappell, D. P. Fairlie, P. Young, M. Trau, Anal. Biochem. 2008, 376, 151.
- 8 A. Nanthakumar, R. T. Pon, A. Mazumder, S. Yu, A. Watson, Bioconjugate Chem. 2000, 11, 282.
- 9 R. H. Scott, S. Balasubramanian, Bioorg. Med. Chem. Lett. 1997, 7, 1567.
- 10 B. J. Battersby, D. Bryant, W. Meutermans, D. Matthews, M. L. Smythe, M. Trau, J. Am. Chem. Soc. 2000, 122, 2138.
- 11 L. Grøndahl, B. J. Battersby, D. Bryant, M. Trau, Langmuir 2000, 16, 9709.
- 12 M. Trau, B. J. Battersby, Adv. Mater. 2001, 13, 975.
- 13 B. J. Battersby, G. A. Lawrie, M. Trau, Drug Discovery Today 2001, 6, 19.
- 14 B. J. Battersby, M. Trau, Trends Biotechnol. 2002, 20, 167.
- 15 W. B. Wu, M. L. Wang, Y. M. Sun, W. Huang, Y. P. Cui, C. X. Xu, Opt. Mater. 2008, 30, 1803.
- 16 L. Wang, W. Tan, Nano Lett. 2006, 6, 84.
- 17 E. A. Jares-Erijman, T. M. Jovin, Nat. Biotechnol. 2003, 21, 1387.
- 18 M. E. Dickinson, G. Bearman, S. Tille, R. Lansford, S. E. Fraser, Biotechniques 2001, 31, 1272.
- 19 G. Lawrie, L. Grondahl, B. Battersby, I. Keen, M. Lorentzen, P. Surawski, M. Trau, Langmuir 2006, 22, 497.
- 20 R. M. Ottenbrite, J. S. Wall, J. Am. Ceram. Soc. 2000, 83, 3214.
- 21 L. M. Rossi, L. Shi, F. H. Quina, Z. Rosenzweig, Langmuir 2005, 21, 4277.
- 22 T. J. Chambers, R. C. Weir, A. Grakoui, D. W. McCourt, J. F. Bazan, R. J. Fletterick, C. M. Rice, Proc. Natl. Acad. Sci. USA 1990, 87, 8898.
- 23 K. J. Chappell, M. J. Stoermer, D. P. Fairlie, P. R. Young, J. Biol. Chem. 2006, 281, 38448.
- 24 S. A. Shiryaev, I. A. Kozlov, B. I. Ratnikov, J. W. Smith, M. Lebl, A. Y. Strongin, Biochem. J. 2007, 401, 743.
- 25 M. Han, X. Gao, J. Z. Su, S. Nie, Nat. Biotechnol. 2001, 19, 631.
- 26 S. L. Diamond, Curr. Opin. Chem. Biol. 2007, 11, 46.
- 27 R. M. Clegg, O. Holub, C. Gohlke, Methods Enzymol. 2003, 360, 509.
