The structure of the extended E2 DNA-binding domain of the bovine papillomavirus-1
Ludmila Leroy
Laboratório de Cristalografia, Physics Department, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Search for more papers by this authorJoão Alexandre Ribeiro Gonçalves Barbosa
Laboratory of Molecular Biophysics, Department of Celular Biology, Universidade de Brasília, Brasília, Brazil
Search for more papers by this authorGonzalo de Prat-Gay
Fundación Instituto Leloir, CABA, Buenos Aires, Argentina
Search for more papers by this authorIgor Polikarpov
Instituto de Física de São Carlos, Universidade de São Paulo, São Calos, Brazil
Search for more papers by this authorCorresponding Author
Carlos Basílio Pinheiro
Laboratório de Cristalografia, Physics Department, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Correspondence
Carlos Basílio Pinheiro, Universidade Federal de Minas Gerais, Departamento de Física – ICEx, Av. Antônio Carlos, 6627, Belo Horizonte 31270-901, Minas Gerais, Brazil.
Email: [email protected]
Search for more papers by this authorLudmila Leroy
Laboratório de Cristalografia, Physics Department, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Search for more papers by this authorJoão Alexandre Ribeiro Gonçalves Barbosa
Laboratory of Molecular Biophysics, Department of Celular Biology, Universidade de Brasília, Brasília, Brazil
Search for more papers by this authorGonzalo de Prat-Gay
Fundación Instituto Leloir, CABA, Buenos Aires, Argentina
Search for more papers by this authorIgor Polikarpov
Instituto de Física de São Carlos, Universidade de São Paulo, São Calos, Brazil
Search for more papers by this authorCorresponding Author
Carlos Basílio Pinheiro
Laboratório de Cristalografia, Physics Department, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Correspondence
Carlos Basílio Pinheiro, Universidade Federal de Minas Gerais, Departamento de Física – ICEx, Av. Antônio Carlos, 6627, Belo Horizonte 31270-901, Minas Gerais, Brazil.
Email: [email protected]
Search for more papers by this authorFunding information: Conselho Nacional de Desenvolvimento Científico e Tecnológico, Grant/Award Numbers: 309086/2016-7, 477789/2008-0
Abstract
Bovine papillomavirus proteins were extensively studied as a prototype for the human papillomavirus. Here, the crystal structure of the extended E2 DNA-binding domain of the dominant transcription regulator from the bovine papillomavirus strain 1 is described in the space group P3121. We found two protein functional dimers packed in the asymmetric unit. This new protein arrangement inside the crystal led to the reduction of the mobility of a previously unobserved loop directly involved in the protein-DNA interaction, which was then modeled for the first time.
CONFLICT OF INTEREST
The authors declare no conflict of interest.
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