Characterization and optimization of de-esterified Tragacanth-chitosan nanocomposite as a potential carrier for oral delivery of insulin: In vitro and ex vivo studies
Touraj Shirzadian
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran
Search for more papers by this authorCorresponding Author
Mohammad Sadegh Nourbakhsh
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran
Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, Iran
Correspondence
Mohammad Sadegh Nourbakhsh, Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, Iran.
Email: [email protected]
Search for more papers by this authorAli Fattahi
Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Search for more papers by this authorGholamreza Bahrami
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Search for more papers by this authorGhobad Mohammadi
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Search for more papers by this authorTouraj Shirzadian
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran
Search for more papers by this authorCorresponding Author
Mohammad Sadegh Nourbakhsh
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran
Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, Iran
Correspondence
Mohammad Sadegh Nourbakhsh, Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, Iran.
Email: [email protected]
Search for more papers by this authorAli Fattahi
Medical Biology Research Center, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Search for more papers by this authorGholamreza Bahrami
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Search for more papers by this authorGhobad Mohammadi
Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
Search for more papers by this authorFunding information: Kermanshah University of Medical Sciences, Grant/Award Number: 94589
Abstract
Oral administration of insulin is one of the most challenging topics within this area, because insulin is degraded in stomach before it enters the bloodstream. In this study, for the first time, a nano-carrier for controlled and targeted oral delivery of insulin was developed using de-esterified Tragacanth and chitosan. The fabricated nanoparticles were synthesized using coacervation technique and their properties were optimized using response surface methodology. The effect of experimental variables on the particle size and loading efficiency was examined. In addition, the interactions between components were analyzed using Fourier transform infrared. The thermal stability of nanoparticles was studied by thermal gravimetric analysis. The insulin loading efficiency was measured and in vitro release profile and ex vivo insulin permeability was determined. Optimized nanoparticles showed spherical shape with a size less than 200 nm and zeta potential of +17 mV. Owing to their nanoscale dimensions and mucoadhesiveness, nanoparticles were synthesized using medium molecular weight of Chitosan. The insulin loading efficacy for the system was 6.4%, released under simulated gastrointestinal conditions in a pH-dependent manner. Based on all of the obtained results, it can be concluded that these nanoparticles can potentially be utilized as a carrier for the oral insulin delivery.
CONFLICT OF INTEREST
The authors assert no conflict of interest, financial or otherwise.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| jbma37202-sup-0001-Tables.docxWord 2007 document , 30.3 KB | Table S1 The set of experiments designed by RSM and actual values of responses (size and loading content) Table S2. ANOVA results for prediction of size of nanoparticles. Table S3. ANOVA results for prediction of loading content. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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