In vitro cytotoxicity evaluation of cadmium by label-free holographic microscopy
Corresponding Author
Martina Mugnano
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Correspondence
Martina Mugnano, CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy.
Email: [email protected]
Search for more papers by this authorPasquale Memmolo
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorLisa Miccio
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorSimonetta Grilli
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorFrancesco Merola
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorAlejandro Calabuig
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorAlessia Bramanti
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Department of Physical Sciences and Technologies of Matter (DSFTM), IRCCS Centre for Neuroscience Bonino-Pulejo, Messina, Italy
Search for more papers by this authorEmanuela Mazzon
Department of Physical Sciences and Technologies of Matter (DSFTM), IRCCS Centre for Neuroscience Bonino-Pulejo, Messina, Italy
Search for more papers by this authorPietro Ferraro
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorCorresponding Author
Martina Mugnano
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Correspondence
Martina Mugnano, CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Via Campi Flegrei 34, 80078 Pozzuoli (NA), Italy.
Email: [email protected]
Search for more papers by this authorPasquale Memmolo
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorLisa Miccio
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorSimonetta Grilli
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorFrancesco Merola
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorAlejandro Calabuig
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorAlessia Bramanti
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Department of Physical Sciences and Technologies of Matter (DSFTM), IRCCS Centre for Neuroscience Bonino-Pulejo, Messina, Italy
Search for more papers by this authorEmanuela Mazzon
Department of Physical Sciences and Technologies of Matter (DSFTM), IRCCS Centre for Neuroscience Bonino-Pulejo, Messina, Italy
Search for more papers by this authorPietro Ferraro
Department of Physical Sciences and Technologies of Matter (DSFTM), CNR, Institute of Applied Science & Intelligent Systems (CNR-ISASI), Pozzuoli, Italy
Search for more papers by this authorAbstract
Among all environmental pollutants, the toxic heavy metal cadmium is considered as a human carcinogen. Cadmium may induce cell death by apoptosis in various cell types, although the underlying mechanisms are still unclear. In this paper we show how a label-free digital holography (DH)-based technique is able to quantify the evolution of key biophysical parameters of cells during the exposure to cadmium for the first time. Murine embryonic fibroblasts NIH 3T3 are chosen here as cellular model for studying the cadmium effects. The results demonstrate that DH is able to retrieve the temporal evolution of different key parameters such as cell volume, projected area, cell thickness and dry mass, thus providing a full quantitative characterization of the cell physical behaviour during cadmium exposure. Our results show that the label-free character of the technique would allow biologists to perform systematic and reliable studies on cell death process induced by cadmium and we believe that more in general this can be easily extended to others heavy metals, thus avoiding the time-consuming, expensive and invasive label-based procedures used nowadays in the field. In fact, pollution by heavy metals is severe issue that needs rapid and reliable methods to be settled.
Supporting Information
| Filename | Description |
|---|---|
| jbio201800099-sup-0001-VideoS1.avivideo/avi, 5.5 MB | Movie S1 The morphology evolution during cadmium exposure. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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